The crosstalk between microtubules, actin and membranes shapes cell                    division

Rizzelli, Francesca; Malabarba, Maria Grazia; Sigismund, Sara; Mapelli, Marina

doi:10.1098/rsob.190314

Cited by 34 publications

(42 citation statements)

References 242 publications

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“…In summary, the molecular network by BRWD3 may underlay the molecular mechanism on alteration of cytoskeleton reorganization, cell shape and motility. The cell shape changes due to microtubules contact the grid and tensile actomyosin cortex rounded-up underneath the plasm membrane [26]. Our observations support the view that the cytoskeleton should be considered as a uni ed system to exert its functions in the development and pathogenesis of complex disorders [27].…”

Section: Discussionsupporting

confidence: 76%

Integrative Networks by BRWD3 Regulates Cytoskeleton Organization and Cell Motility

Li¹,

Wang²,

Cui³

et al. 2020

Preprint

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BackgroundEukaryotic cytoskeleton forms and keeps cell shape, transports intracellular particles and organelles, determines cell motility and other important cellular events. A large number of regulators of cytoskeleton organization have been identified, but the detailed regulatory mechanism still remains obscure. Previous reports suggest that BRWD3 may be a regulator of cytoskeleton organization in Drosophila melanogaster, and influences cell shape. Therefore, we investigated the molecular network of BRWD3 regulating cytoskeleton organization.ResultsIn this study, we observed the alteration of cell shape, cell motility, and proliferation when BRWD3 was knocked down in MCF-7 and MDA-MB-231 cell lines. The cells were rounded, cell motility decreased when BRWD3 was knocked down. Using chromatin immunoprecipitation combining with sequencing, we found that BRWD3 influenced the cytoskeleton organization, cell shape, and cell motility through regulating expression of the cytoskeleton associative genes including ARF1, ABI2, ARPC3, ARPC1A, RHOC, MEF2C, and VIM.ConclusionsA molecular network by BRWD3 is sketched to elucidate that BRWD3 may not only regulate actin filament but also regulate microtubule and intermediate filament-based cytoskeleton organization. These efforts provide an overview of a BRWD3 network regulating cytoskeleton organization, cell shape and motility, and allow a better understanding of cytoskeleton (re)organization and pathogenesis of mental retardation X-linked 93 and relative carcinomas.

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Section: Discussionsupporting

confidence: 76%

Integrative Networks by BRWD3 Regulates Cytoskeleton Organization and Cell Motility

Li¹,

Wang²,

Cui³

et al. 2020

Preprint

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“…Apart from moving along microtubules for transport purposes, dynein can be anchored at subcellular sites to exert pulling forces on other structures via microtubules [78][79][80]. One of the best studied examples is the anchoring of dynein by a trimeric complex consisting of the GDP-loaded Gαi subunit of heterotrimeric G-proteins, the adapter protein LGN (leucine-glycine-asparagine), and the dynein-binding nuclear mitotic apparatus protein (NuMA) to the mitotic cell cortex [81][82][83]. There, dynein exerts forces on astral microtubules to shape and position the bipolar spindle [84].…”

Section: Control Of Microtubule Functionmentioning

confidence: 99%

Microtubule Organization in Striated Muscle Cells

Becker

Leone

Engel

2020

Cells

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Distinctly organized microtubule networks contribute to the function of differentiated cell types such as neurons, epithelial cells, skeletal myotubes, and cardiomyocytes. In striated (i.e., skeletal and cardiac) muscle cells, the nuclear envelope acts as the dominant microtubule-organizing center (MTOC) and the function of the centrosome—the canonical MTOC of mammalian cells—is attenuated, a common feature of differentiated cell types. We summarize the mechanisms known to underlie MTOC formation at the nuclear envelope, discuss the significance of the nuclear envelope MTOC for muscle function and cell cycle progression, and outline potential mechanisms of centrosome attenuation.

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“…Mitosis is a very disruptive process, during which cells are rounded, duplicated chromosomes condense, the cytoskeleton is reorganized, and more importantly, cellular endomembrane system undergoes dramatic fragmentation and remodeling [26,27]. It has long been recognized that endocytosis is repressed in mitosis and many endocytic proteins, including clathrin, caveolin-1, and several ESCRT complex components, change their normal distribution in different stages of mitosis [28][29][30][31][32].…”

Section: Mitotic Moonlighting Functions Of Endocytic Proteinsmentioning

confidence: 99%

Functional reciprocity of proteins involved in mitosis and endocytosis

et al. 2020

The FEBS Journal

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Mitosis and endocytosis are two fundamental cellular processes essential for maintaining a eukaryotic life. Mitosis partitions duplicated chromatin enveloped in the nuclear membrane into two new cells, whereas endocytosis takes in extracellular substances through membrane invagination. These two processes are spatiotemporally separated and seemingly unrelated. However, recent studies have uncovered that endocytic proteins have moonlighting functions in mitosis, and mitotic complexes manifest additional roles in endocytosis. In this review, we summarize important proteins or protein complexes that participate in both processes, compare their mechanism of action, and discuss the rationale behind this multifunctionality. We also speculate on the possible origin of the functional reciprocity from an evolutionary perspective. An introduction to mitosis and endocytic pathwaysEukaryotic cells rely on endocytosis to uptake extracellular materials and their own plasma membrane components, in order to maintain homeostasis and respond to the varying environment [1-3]; they use the process of mitosis to achieve self-duplication and cell fate specification when differentiating [1,4]. These two Abbreviations AP2, adaptor protein-2; APC/C, anaphase-promoting complex/cyclosome; CCP, clathrin-coated pit; CCV, clathrin-coated vesicle; CHC, clathrin heavy chains; ch-TOG, colonic and hepatic tumor overexpressed gene; CLC, clathrin light chain; CME, clathrin-mediated endocytosis; CPC, chromosome passenger complex;

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The crosstalk between microtubules, actin and membranes shapes cell division

Cited by 34 publications

References 242 publications

Integrative Networks by BRWD3 Regulates Cytoskeleton Organization and Cell Motility

Integrative Networks by BRWD3 Regulates Cytoskeleton Organization and Cell Motility

Microtubule Organization in Striated Muscle Cells

Functional reciprocity of proteins involved in mitosis and endocytosis

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