The CRISPR/Cas9 System for Crop Improvement: Progress and Prospects

Leong, Kah-Yung Bernard; Chan, Yee-Han; Abdullah, Wan Muhamad Asrul Nizam Wan; Lim, Swee Hua Erin; Lai, Kok-Song

doi:10.5772/intechopen.75024

Cited by 3 publications

(2 citation statements)

References 67 publications

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“…Compared to other gene-editing methods such as TALEN or zinc finger nuclease (ZFN), CRISPR/Cas9 has advantages in simple target design, multiplexed mutation, versatility, and efficiency (Leong et al, 2018). There have been numerous proof-of-concept experiments conducted using the CRISPR/Cas9 gene-editing platform (Lawrenson et al, 2015), but few developments have reached commercialization (Nonaka et al, 2017).…”

Section: Current Progress Using Crispr/cas9 Systemmentioning

confidence: 99%

Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance

Karunarathne

Walker

Sharma

et al. 2023

J. Zhejiang Univ. Sci. B

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Abiotic stresses, predominately drought, heat, salinity, cold, and waterlogging, adversely affect cereal crops. They limit barley production worldwide and cause huge economic losses. In barley, functional genes under various stresses have been identified over the years and genetic improvement to stress tolerance has taken a new turn with the introduction of modern gene-editing platforms. In particular, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is a robust and versatile tool for precise mutation creation and trait improvement. In this review, we highlight the stress-affected regions and the corresponding economic losses among the main barley producers. We collate about 150 key genes associated with stress tolerance and combine them into a single physical map for potential breeding practices. We also overview the applications of precise base editing, prime editing, and multiplexing technologies for targeted trait modification, and discuss current challenges including high-throughput mutant genotyping and genotype dependency in genetic transformation to promote commercial breeding. The listed genes counteract key stresses such as drought, salinity, and nutrient deficiency, and the potential application of the respective gene-editing technologies will provide insight into barley improvement for climate resilience.

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Section: Current Progress Using Crispr/cas9 Systemmentioning

confidence: 99%

Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance

Karunarathne

Walker

Sharma

et al. 2023

J. Zhejiang Univ. Sci. B

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show abstract

“…Both Cas9 and sgRNA expression cassettes are assembled in vectors to perform further genome editing procedures. Before conducting a final genome editing step, protoplasts are transformed with the CRISPR to analyze and validate the sgRNA activity [58]. Next, a PCR or restriction enzyme digestion step is employed to select the active CRISPR.…”

Section: Crispr/cas9 and Cpf1 In Genome Editingmentioning

confidence: 99%

Novel Plant Breeding Techniques Shake Hands with Cereals to Increase Production

Haroon

Wang

Afzal

et al. 2022

Plants

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Cereals are the main source of human food on our planet. The ever-increasing food demand, continuously changing environment, and diseases of cereal crops have made adequate production a challenging task for feeding the ever-increasing population. Plant breeders are striving their hardest to increase production by manipulating conventional breeding methods based on the biology of plants, either self-pollinating or cross-pollinating. However, traditional approaches take a decade, space, and inputs in order to make crosses and release improved varieties. Recent advancements in genome editing tools (GETs) have increased the possibility of precise and rapid genome editing. New GETs such as CRISPR/Cas9, CRISPR/Cpf1, prime editing, base editing, dCas9 epigenetic modification, and several other transgene-free genome editing approaches are available to fill the lacuna of selection cycles and limited genetic diversity. Over the last few years, these technologies have led to revolutionary developments and researchers have quickly attained remarkable achievements. However, GETs are associated with various bottlenecks that prevent the scaling development of new varieties that can be dealt with by integrating the GETs with the improved conventional breeding methods such as speed breeding, which would take plant breeding to the next level. In this review, we have summarized all these traditional, molecular, and integrated approaches to speed up the breeding procedure of cereals.

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Biofuels and Sustainability

Eswaran¹,

Parameswaran²,

Johnson³

2021

Methods in Molecular Biology

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translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Regarding these commercial rights a non-exclusive license has been granted to the publisher. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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The CRISPR/Cas9 System for Crop Improvement: Progress and Prospects

Cited by 3 publications

References 67 publications

Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance

Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance

Novel Plant Breeding Techniques Shake Hands with Cereals to Increase Production

Biofuels and Sustainability

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