Biofuels and Sustainability

Eswaran, Nalini; Parameswaran, S.; Johnson, T. Sudhakar

doi:10.1007/978-1-0716-1323-8_20

Cited by 16 publications

(6 citation statements)

References 110 publications

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“…The growing world population is expected to cause the depletion of energy resources unless we drastically change our lifestyle, such as stopping the use of fossil fuels. To achieve a sustainable society, biofuels are expected to replace fossil fuels [ 99 , 100 ]. In molecular display systems, various foreign enzymes are immobilized on the yeast cell surface.…”

Section: Applications As a Whole-cell Catalyst In Energy Productionmentioning

confidence: 99%

Progress of Molecular Display Technology Using Saccharomyces cerevisiae to Achieve Sustainable Development Goals

Shibasaki

Ueda

2023

Microorganisms

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In the long history of microorganism use, yeasts have been developed as hosts for producing biologically active compounds or for conventional fermentation. Since the introduction of genetic engineering, recombinant proteins have been designed and produced using yeast or bacterial cells. Yeasts have the unique property of expressing genes derived from both prokaryotes and eukaryotes. Saccharomyces cerevisiae is one of the well-studied yeasts in genetic engineering. Recently, molecular display technology, which involves a protein-producing system on the yeast cell surface, has been established. Using this technology, designed proteins can be displayed on the cell surface, and novel abilities are endowed to the host yeast strain. This review summarizes various molecular yeast display technologies and their principles and applications. Moreover, S. cerevisiae laboratory strains generated using molecular display technology for sustainable development are described. Each application of a molecular displayed yeast cell is also associated with the corresponding Sustainable Development Goals of the United Nations.

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Section: Applications As a Whole-cell Catalyst In Energy Productionmentioning

confidence: 99%

Progress of Molecular Display Technology Using Saccharomyces cerevisiae to Achieve Sustainable Development Goals

Shibasaki

Ueda

2023

Microorganisms

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“…The increasing demand for fossil fuels caused by burgeoning anthropogenic activities and rapid economic growth provoked wicked environmental issues and resource depletion [3,4], which is a direct boost to reconstruct the energy structure, develop and industrialize renewable biofuels [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Bioethanol Production from Decaying Oranges and Pineapple Juice Using Ethanol Tolerant-Yeast

Omoolorun, J. B.,

Afolabi, F. T.,

Olufemi, S. E.

et al. 2023

JABB

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Scientists across the globe ought to harness ways of getting alternative sources of energy which will be renewable, sustainable, efficient, eco-friendly, and cost effective because of the global energy crises owing to the cost of production, transportation and distribution of fossil fuel products. In Nigeria, decaying fruits always constitute a major environmental pollution during the harvesting season. This study screened, selected the best starter, and produced bioethanol from the juice obtained from decaying oranges and pineapple through the process of fermentation and distillation. Samples were collected from different locations within Ile-Ife and transported aseptically to the Laboratory. Microbiological and physicochemical assessment of the isolated strains were on yeast maintenance media. The cell biomass, pH, temperature, brix level, titratable acidity, specific gravity and ethanol yield were monitored during fermentation from day zero to day fourteen. Screening of the isolates obtained from a previous study was carried out to select the best starter for the production of bioethanol. S.cerevisae and K. marxianus and showed efficient physico-chemical attributes from the screening of the yeast isolates; a temperature of 30oC and pH 6 was the optimum for the growth of isolates tolerating 20% v/v absolute ethanol. Cultures were inoculated singly and in combination, S. cerevisiae gave the highest reduction in brix level from 2.2o at the onset and it reduced to 0.3o at the 21st day of fermentation while the least reduction was seen in K. marxianus. Mixed culture of S. cerevisiae and K. marxianus gave the highest reduction in brix level from 2.0o at the onset to 0.1o, pH reduced from 4.7- 3.3 while the cell biomass increased, and the temperature increased from 30oC to 34.5oC at the end of fermentation. Titratable acidity in the fermenting fruits juice increased from 0.23 to 1.76, the specific gravity reduced while the alcohol content increased from zero to 25.63 as the fermentation progressed and a reduction on day 21 (1.67) was observed. The use of decaying plant biomass or fruit waste can be a rich and cheaper source of substrate for different yeasts strains endogenous to the biomass for the production of environmental-friendly biofuel. This can also be employed as a waste management option and an alternative solution to environmental pollution and the global energy crises.

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“…The depletion of fossil fuels and the environmental problems caused by their use necessitate the urgent development of new and alternative environmentally friendly energy sources [ 1 ]. Standing out among new energy sources, hydrogen is a completely clean fuel because its combustion only produces water while offering high energy density [ 2 , 3 ]. However, despite the enormous development potential of hydrogen energy, the production and utilization of hydrogen still faces many challenges.…”

Section: Introductionmentioning

confidence: 99%

Optimization of hydrogen production in Enterobacter aerogenes by Complex I peripheral fragments destruction and maeA overexpression

et al. 2023

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As a concentrated energy source with high added value, hydrogen has great development prospects, with special emphasis on sustainable microbial production as a replacement for traditional fossil fuels. In this study, λ-Red recombination was used to alter the activity of Complex I by single and combined knockout of nuoE, nuoF and nuoG. In addition, the conversion of malic to pyruvic acid was promoted by overexpressing the maeA gene, which could increase the content of NADH and formic acid in the bacterial cells. Compared to the original strain, hydrogen production was 65% higher in the optimized strain IAM1183-EFG/M, in which the flux of the formic acid pathway was increased by 257%, the flux of the NADH pathway was increased by 13%, and the content of metabolites also changed significantly. In further bioreactor, the total hydrogen production of the scale-up IAM1183-EFG/M after 44 h of fermentation was 4.76 L, which increased by 18% compared with the starting strain. This study provides a new direction for future exploration of microbial hydrogen production by combinatorial modification of multiple genes. Graphical Abstract

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Biofuels and Sustainability

Cited by 16 publications

References 110 publications

Progress of Molecular Display Technology Using Saccharomyces cerevisiae to Achieve Sustainable Development Goals

Progress of Molecular Display Technology Using Saccharomyces cerevisiae to Achieve Sustainable Development Goals

Bioethanol Production from Decaying Oranges and Pineapple Juice Using Ethanol Tolerant-Yeast

Optimization of hydrogen production in Enterobacter aerogenes by Complex I peripheral fragments destruction and maeA overexpression

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