The coupling of mixotrophic denitrification, dissimilatory nitrate reduction to ammonium (DNRA) and anaerobic ammonium oxidation (anammox) promoting the start-up of anammox by addition of calcium nitrate

Sheng, Hao; Weng, Rui; He, Yan; Zheng, Wei; Yang, Yanmei; Chen, Jinghan; Huang, Minsheng; Zhou, Guanghui

doi:10.1016/j.biortech.2021.125822

Cited by 29 publications

(4 citation statements)

References 40 publications

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“…The media can provide a suitable surface on which a biofilm can form while adhering bacteria may aid in COD removal [ 93 , 140 ]. One of the typical leachate characteristics is it has an extremely low BOD 5 /COD ratio (<0.1), which can be said that most of the organic compounds in leachate are non-biodegradable [ 17 , 74 , 123 ] The low percentage of COD removal during operation is possible because of the higher concentration of non-biodegradable organic compounds in the leachate [ 24 , 39 , 65 ]. The initial leachate BOD/COD value is 0.3–0.4, hence, it is classified as biodegradable.…”

Section: Resultsmentioning

confidence: 99%

Enhanced leachate phytodetoxification test combined with plants and rhizobacteria bioaugmentation

Arliyani

Tangahu²,

Mangkoedihardjo³

et al. 2023

Heliyon

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Section: Resultsmentioning

confidence: 99%

Enhanced leachate phytodetoxification test combined with plants and rhizobacteria bioaugmentation

Arliyani

Tangahu²,

Mangkoedihardjo³

et al. 2023

Heliyon

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“…Previous studies have reported the presence and increased replication of DNRA bacteria to coincide with decreased reactor performance ( Keren et al, 2020 ). However, DNRA bacteria have also been shown to form symbiotic interactions with anammox bacteria, promoting robust reactor performance ( Li et al, 2020 ; Sheng et al, 2021 ). Many anammox species can also perform DNRA using simple organic acids as substrates ( Castro-Barros et al, 2017 ), further complicating the characterization of the interplay between these metabolisms.…”

Section: Discussionmentioning

confidence: 99%

“…DNRA can provide anammox bacteria with nitrite through the reduction of nitrate via nar and nap nitrate reductases, or ammonium through the reduction of nitrite with nrfAH nitrite reductases. This steady flow of material exchanges can result in enhanced nitrogen removal in a variety of ecosystems ( Ahmad et al, 2021 ; Sheng et al, 2021 ). Despite this, DNRA can still actively compete with anammox for nitrite, and even potentially destabilize aggregates through overconsumption of EPS, leading to anammox cell death ( Keren et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Synergistic interactions between anammox and dissimilatory nitrate reducing bacteria sustains reactor performance across variable nitrogen loading ratios

et al. 2023

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Anaerobic ammonium oxidizing (anammox) bacteria are utilized for high efficiency nitrogen removal from nitrogen-laden sidestreams in wastewater treatment plants. The anammox bacteria form a variety of competitive and mutualistic interactions with heterotrophic bacteria that often employ denitrification or dissimilatory nitrate reduction to ammonium (DNRA) for energy generation. These interactions can be heavily influenced by the influent ratio of ammonium to nitrite, NH4+:NO2−, where deviations from the widely acknowledged stoichiometric ratio (1:1.32) have been demonstrated to have deleterious effects on anammox efficiency. Thus, it is important to understand how variable NH4+:NO2− ratios impact the microbial ecology of anammox reactors. We observed the response of the microbial community in a lab scale anammox membrane bioreactor (MBR) to changes in the influent NH4+:NO2− ratio using both 16S rRNA gene and shotgun metagenomic sequencing. Ammonium removal efficiency decreased from 99.77 ± 0.04% when the ratio was 1:1.32 (prior to day 89) to 90.85 ± 0.29% when the ratio was decreased to 1:1.1 (day 89–202) and 90.14 ± 0.09% when the ratio was changed to 1:1.13 (day 169–200). Over this same timespan, the overall nitrogen removal efficiency (NRE) remained relatively unchanged (85.26 ± 0.01% from day 0–89, compared to 85.49 ± 0.01% from day 89–169, and 83.04 ± 0.01% from day 169–200). When the ratio was slightly increased to 1:1.17–1:1.2 (day 202–253), the ammonium removal efficiency increased to 97.28 ± 0.45% and the NRE increased to 88.21 ± 0.01%. Analysis of 16 S rRNA gene sequences demonstrated increased relative abundance of taxa belonging to Bacteroidetes, Chloroflexi, and Ignavibacteriae over the course of the experiment. The relative abundance of Planctomycetes, the phylum to which anammox bacteria belong, decreased from 77.19% at the beginning of the experiment to 12.24% by the end of the experiment. Analysis of metagenome assembled genomes (MAGs) indicated increased abundance of bacteria with nrfAH genes used for DNRA after the introduction of lower influent NH4+:NO2− ratios. The high relative abundance of DNRA bacteria coinciding with sustained bioreactor performance indicates a mutualistic relationship between the anammox and DNRA bacteria. Understanding these interactions could support more robust bioreactor operation at variable nitrogen loading ratios.

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“…They probably inhabit the oxygen-depleted zones of aerated biofilters ( Mulder et al, 1995 ), where an anoxic environment is created by AOA, AOB, and heterotrophic bacteria. AnAOB use nitrite and ammonia generated by heterotrophic denitrification and the dissimilatory nitrate reduction process ( Hao et al, 2021 ) ( Figure 1A ), where nitrate concentration is high but ammonia and nitrite are scarce. Alternatively, AnAOB can use nitrite produced by AOA and AOB and ammonia generated by heterotrophic ammonification, in what is called the one-stage nitritation/anammox process ( Li et al, 2018 ) ( Figure 1B ).…”

Section: Opportunity For Nitrogen Removal By Ammonia-oxidizing Archaea and Anaerobic Ammonia-oxidizing Bacteria In Aquaculture Pondsmentioning

confidence: 99%

A Review of Ammonia-Oxidizing Archaea and Anaerobic Ammonia-Oxidizing Bacteria in the Aquaculture Pond Environment in China

Liu

Cheng

et al. 2021

Front. Microbiol.

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The excessive ammonia produced in pond aquaculture processes cannot be ignored. In this review, we present the distribution and diversity of ammonia-oxidizing archaea (AOA) and anaerobic ammonia-oxidizing bacteria (AnAOB) in the pond environment. Combined with environmental conditions, we analyze the advantages of AOA and AnAOB in aquaculture water treatment and discuss the current situation of pond water treatment engineering involving these microbes. AOA and AnAOB play an important role in the nitrogen removal process of aquaculture pond water, especially in seasonal low temperatures and anoxic sediment layers. Finally, we prospect the application of bioreactors to purify pond aquaculture water using AOA and AnAOB, in autotrophic nitrogen removal, which can reduce the production of greenhouse gases (such as nitrous oxide) and is conducive to the development of environmentally sustainable pond aquaculture.

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The coupling of mixotrophic denitrification, dissimilatory nitrate reduction to ammonium (DNRA) and anaerobic ammonium oxidation (anammox) promoting the start-up of anammox by addition of calcium nitrate

Cited by 29 publications

References 40 publications

Enhanced leachate phytodetoxification test combined with plants and rhizobacteria bioaugmentation

Enhanced leachate phytodetoxification test combined with plants and rhizobacteria bioaugmentation

Synergistic interactions between anammox and dissimilatory nitrate reducing bacteria sustains reactor performance across variable nitrogen loading ratios

A Review of Ammonia-Oxidizing Archaea and Anaerobic Ammonia-Oxidizing Bacteria in the Aquaculture Pond Environment in China

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