The country home, by E. P. Powell.

Powell, Edward

doi:10.5962/bhl.title.28955

Cited by 24 publications

(38 citation statements)

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“…FVIII mRNA levels in LSECs and hepatocytes were quantitated by a modification of published competitive RT-PCR procedures (13)(14)(15). In this method, a known concentration of fVIII-specific cRNA is added to the RT reaction, which produces a cDNA that competes for the native fVIII cDNA during PCR amplification.…”

mentioning

confidence: 99%

Expression of Factor VIII by Murine Liver Sinusoidal Endothelial Cells

Healey

Waller

et al. 1999

Journal of Biological Chemistry

183

168

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Factor VIII (fVIII) is the procoagulant plasma glycoprotein that is missing or decreased in hemophilia A. The cellular origin of fVIII synthesis is controversial. Liver transplantation cures hemophilia A, demonstrating that the liver is a major site of fVIII synthesis. We detected fVIII mRNA in purified populations of murine liver sinusoidal endothelial cells (LSECs) and hepatocytes, but not Kupffer cells. LSECs and hepatocytes contained comparable numbers of fVIII mRNA (40 and 70 transcripts per cell, respectively) by quantitative competitive reverse transcriptase-polymerase chain reaction analysis. There was not detectable mRNA for factor IX, a hepatocyte marker, in the LSEC preparation, nor was there detectable mRNA for von Willebrand factor, an endothelial cell marker, in the hepatocyte preparation. This excludes the possibility that detectable fVIII mRNA is due to cross-contamination in the hepatocyte or LSEC preparations. Primary cultures of LSECs were established in which fVIII mRNA levels were indistinguishable from purified LSECs. LSECs secreted active fVIII into the culture medium. This finding represents the first demonstration of homologous expression of fVIII mRNA and protein in cell culture and should facilitate studies of fVIII gene regulation. Additionally, LSECs potentially are targets for a fVIII transgene during gene therapy of hemophilia A.The site of the cellular origin for the biosynthesis of fVIII 1 remains controversial despite studies that date back nearly 50 years (see Refs. 1 and 2, for reviews). Human and canine hemophilia A are cured by liver transplantation (3-6), which demonstrates that the liver contributes significantly to fVIII synthesis. Hepatocytes (7,8), liver sinusoidal endothelial cells (LSECs) (9 -11), or both (12), have been proposed as sites of fVIII synthesis. In this study, we isolated hepatocytes, LSECs, and Kupffer cells and measured steady-state levels of fVIII mRNA in these preparations. Our results indicate that both LSECs and hepatocytes synthesize fVIII mRNA. Additionally, LSECs in culture secrete active fVIII, providing a model for studies of the regulation of homologous fVIII gene expression. EXPERIMENTAL PROCEDURESMaterials-Gey's balanced salt solution, Hank's balanced salt solution (HBSS), Dulbecco's phosphate-buffered saline (PBS), Liver Digest Medium, DMEM/F-12 medium, and AIM-V medium were purchased from Life Technologies, Inc. (Gaithersburg, MD). Penicillin (50 units/ ml) and streptomycin (50 g/ml) were added to DMEM/F-12 medium. Collagenase (type IV), gelatin, and dibutyryl cAMP were purchased from Sigma. DNase I was purchased from Roche Molecular Biochemicals (Indianapolis, IN). The following murine monoclonal IgG 1 antibodies were purchased from Pharmigen (San Diego, CA): FITC-conjugated anti-PECAM-1 (anti-CD31), FITC-conjugated anti-VCAM-1 (anti-CD106), PE-conjugated anti-ICAM-1 (anti-CD54), the corresponding FITC-conjugated-and PE-conjugated isotype-specific control antibodies, and biotinylated anti-ICAM-1. FITC-conjugated wheat germ agglutinin was ...

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mentioning

confidence: 99%

Expression of Factor VIII by Murine Liver Sinusoidal Endothelial Cells

Healey

Waller

et al. 1999

Journal of Biological Chemistry

183

168

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“…identification of Gypsies, disidentification from Gypsies and avoidance of all identification with Gypsies are the necessary conditions for the maintenance of power differentials, the feeling of superior human virtue in the "we-image" of the settled population, and the resultant stigmatization. 40 The Approach of the Opposition Parties…”

Section: Romani Opening In Turkey 607mentioning

confidence: 99%

The Romani Opening in Turkey: Antidiscrimination?

Gençoğlu-Onbaşi

2012

Turkish Studies

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This study is a critical analysis of the Romani Opening in Turkey that was initiated by the government in the late 2009 as a project aiming to end discrimination against Roma in Turkey. Although this is a very important move considering the longlasting ignorance of the problems of Roma in Turkey, there are important problems related with the presentation of the project and discussions revolving around it. As an outcome of these problems this project has become a case where one can observe how antidiscrimination initiatives/strategies/policies can be discriminatory in their implementation. This study also argues that this paradoxical situation can only be figured out by reflection on the notion of power. A reflection on power, in turn, will necessarily lead to a re-thinking on democracy.

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“…Moreover, "[b]y being a part of [the] world, and by not withdrawing, America makes [the] world safer, more productive, more prosperous, and more humane" [239]. Schlesinger sounds similar themes.…”

Section: Multilateralismmentioning

confidence: 99%

“…The Base Force was "designed to be capability oriented, to provide a capability-a tool box ... to deal with the uncertainties of the future, to handle Downloaded by [New York University] at 16:05 11 April 2015 the next crisis in whatever region it arises" [254]. It was "the minimum force the Nation needs to accomplish [a] new strategy [centered on regional conflicts] and also to be consistent with the funding [the armed forces] are likely to get from the Congress" [255].…”

Section: Multilateralismmentioning

confidence: 99%