The Cotton <i>ACTIN1</i> Gene Is Functionally Expressed in Fibers and Participates in Fiber Elongation

Li, Xue Bao; Fan, Xiao Ping; Wang, Xiu Lan; Cai, Lin; Yang, Wei

doi:10.1105/tpc.104.029629

Cited by 331 publications

(287 citation statements)

References 65 publications

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“…EU604080) was used as a standard control in the RT-PCR. A two-step RT-PCR procedure was performed in all experiments using a method described earlier (Li et al, 2005). In brief, total RNA was reverse transcribed into cDNA and used as templates in real-time PCR with gene-specific primers (Supplemental Table S1).…”

Section: Quantitative Rt-pcr Analysismentioning

confidence: 99%

“…Until now, however, the limited numbers of cotton genes involved in fiber development have been characterized, and the molecular mechanism controlling fiber development still remains unclear. A previous study revealed that GhACT1, expressed predominantly in fiber cells, participates in fiber elongation (Li et al, 2005). GhDET2 plays a role in the initiation and elongation of fiber cells (Luo et al, 2007).…”

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confidence: 96%

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A Fasciclin-Like Arabinogalactan Protein, GhFLA1, Is Involved in Fiber Initiation and Elongation of Cotton

et al. 2013

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Arabinogalactan proteins (AGPs) are involved in many aspects of plant development. In this study, biochemical and genetic approaches demonstrated that AGPs are abundant in developing fibers and may be involved in fiber initiation and elongation. To further investigate the role of AGPs during fiber development, a fasciclin-like arabinogalactan protein gene (GhFLA1) was identified in cotton (Gossypium hirsutum). Overexpression of GhFLA1 in cotton promoted fiber elongation, leading to an increase in fiber length. In contrast, suppression of GhFLA1 expression in cotton slowed down fiber initiation and elongation. As a result, the mature fibers of the transgenic plants were significantly shorter than those of the wild type. In addition, expression levels of GhFLAs and the genes related to primary cell wall biosynthesis were remarkably enhanced in the GhFLA1 overexpression transgenic fibers, whereas the transcripts of these genes were dramatically reduced in the fibers of GhFLA1 RNA interference plants. An immunostaining assay indicated that both AGP composition and primary cell wall composition were changed in the transgenic fibers. The levels of glucose, arabinose, and galactose were also altered in the primary cell wall of the transgenic fibers compared with those of the wild type. Together, our results suggested that GhFLA1 may function in fiber initiation and elongation by affecting AGP composition and the integrity of the primary cell wall matrix.

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Section: Quantitative Rt-pcr Analysismentioning

confidence: 99%

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confidence: 96%

A Fasciclin-Like Arabinogalactan Protein, GhFLA1, Is Involved in Fiber Initiation and Elongation of Cotton

et al. 2013

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“…In addition, lipid transfer proteins also show higher levels of gene expression in fast elongating fiber cells [8]. Actin is also reported to function in the fiber elongation [9].…”

Section: Introductionmentioning

confidence: 96%

Gene expression and metabolite profiles of cotton fiber during cell elongation and secondary cell wall synthesis

et al. 2007

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Cotton fibers elongate rapidly after initiation of elongation, eventually leading to the deposit of a large amount of cellulose. To reveal features of cotton fiber cells at the fast elongation and the secondary cell wall synthesis stages, we compared the respective transcriptomes and metabolite profiles. Comparative analysis of transcriptomes by cDNA array identified 633 genes that were differentially regulated during fiber development. Principal component analysis (PCA) using expressed genes as variables divided fiber samples into four groups, which are diagnostic of developmental stages. Similar grouping results are also found if we use non-polar or polar metabolites as variables for PCA of developing fibers. Auxin signaling, wall-loosening and lipid metabolism are highly active during fiber elongation, whereas cellulose biosynthesis is predominant and many other metabolic pathways are downregulated at the secondary cell wall synthesis stage. Transcript and metabolite profiles and enzyme activities are consistent in demonstrating a specialization process of cotton fiber development toward cellulose synthesis. These data demonstrate that cotton fiber cell at a certain stage has its own unique feature, and developmental stages of cotton fiber cells can be distinguished by their transcript and metabolite profiles. During the secondary cell wall synthesis stage, metabolic pathways are streamed into cellulose synthesis.

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“…Because fiber length per se is an agronomically important property, much attention has been focused on the molecular mechanisms of fiber elongation (8)(9)(10). In plants, a highly dynamic actin cytoskeleton has emerged as a key determinant of cell morphology.…”

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Parallel up-regulation of the profilin gene family following independent domestication of diploid and allopolyploid cotton ( Gossypium )

Bao

Flagel

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Cotton is remarkable among our major crops in that four species were independently domesticated, two allopolyploids and two diploids. In each case thousands of years of human selection transformed sparsely flowering, perennial shrubs into highly productive crops with seeds bearing the vastly elongated and abundant single-celled hairs that comprise modern cotton fiber. The genetic underpinnings of these transformations are largely unknown, but comparative gene expression profiling experiments have demonstrated up-regulation of profilin accompanying domestication in all three species for which wild forms are known. Profilins are actin monomer binding proteins that are important in cytoskeletal dynamics and in cotton fiber elongation. We show that Gossypium diploids contain six profilin genes (GPRF1-GPRF6), located on four different chromosomes (eight chromosomes in the allopolyploid). All but one profilin (GPRF6) are expressed during cotton fiber development, and both homeologs of GPRF1-GPRF5 are expressed in fibers of the allopolyploids. Remarkably, quantitative RT-PCR and RNAseq data demonstrate that GPRF1-GPRF5 are all up-regulated, in parallel, in the three independently domesticated cottons in comparison with their wild counterparts. This result was additionally supported by iTRAQ proteomic data. In the allopolyploids, there This usage of novel should be fine, since it refers to a novel evolutionary process, not a novel discovery has been novel recruitment of the sixth profilin gene (GPRF6) as a result of domestication. This parallel up-regulation of an entire gene family in multiple species in response to strong directional selection is without precedent and suggests unwitting selection on one or more upstream transcription factors or other proteins that coordinately exercise control over profilin expression.polyploidy | crop evolution | fiber | directional selection

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The Cotton ACTIN1 Gene Is Functionally Expressed in Fibers and Participates in Fiber Elongation

Cited by 331 publications

References 65 publications

A Fasciclin-Like Arabinogalactan Protein, GhFLA1, Is Involved in Fiber Initiation and Elongation of Cotton

A Fasciclin-Like Arabinogalactan Protein, GhFLA1, Is Involved in Fiber Initiation and Elongation of Cotton

Gene expression and metabolite profiles of cotton fiber during cell elongation and secondary cell wall synthesis

Parallel up-regulation of the profilin gene family following independent domestication of diploid and allopolyploid cotton ( Gossypium )

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