The correlation of long non-coding RNA NEAT1 and its targets microRNA (miR)-21, miR-124, and miR-125a with disease risk, severity, and inflammation of allergic rhinitis

Abstract

The present study aimed to investigate the correlation of long non-coding RNA nuclear-enriched abundant transcript 1 (lncRNA NEAT1) with microRNA (miR)-21, miR-124, and miR-125a, and their associations with disease risk, severity, and inflammatory cytokines of allergic rhinitis (AR). Totally 70 AR patients and 70 non-atopic obstructive snoring patients (as controls) were recruited. Inferior turbinate mucosa samples were collected from all participants for lncRNA NEAT1, its targets (miR-21, miR-124, and miR-125a), interleukin (IL)-4, IL-6, IL-10, and IL-17 detection via reverse transcription quantitative polymerase chain reaction. Disease severity of AR patients was assessed using individual nasal symptom score (INSS) and total nasal symptom score (TNSS). LncRNA NEAT1 was upregulated, while miR-21, miR-124, and miR-125a were downregulated in AR patients compared with controls. Additionally, lncRNA NEAT1, miR-21, and miR-125a displayed good values in differentiating AR patients from controls, while miR-124 could only slightly differentiate AR patients from controls. In AR patients, lncRNA NEAT1 was negatively associated with miR-21 and miR-125a, but not miR-124. However, in controls, no correlation of lncRNA NEAT1 with miR-21, miR-124, or miR-125a was observed. Furthermore, in AR patients, lncRNA NEAT1 was positively, while miR-21 and miR-125a was negatively associated with INSS (rhinorrhea, itching, congestion scores), TNSS and inflammatory cytokines; however, correlation of miR-124 with INSS, TNSS, and inflammatory cytokines was slight. LncRNA NEAT1 and its targets (miR-21 and miR-125a) present close correlations with disease risk, severity, and inflammation of AR, suggesting their potential as biomarkers for AR assessment.

Long non‐coding RNA NEAT1 and its targets (microRNA‐21 and microRNA‐125a) in rheumatoid arthritis: Altered expression and potential to monitor disease activity and treatment outcome

“…In our study, we found that lnc-NEAT1 was elevated, while miR-21 Furthermore, we also found that lnc-NEAT1 was negatively correlated with miR-21 and miR-125a, which might be explained by that lnc-NEAT1 could target miR-21 and miR-125a in immunological disease (such as allergic rhinitis), the similar targeting manner might also exist in RA. 7 A previous study has illustrated that lnc-NEAT1 is positively correlated with disease activity in SLE patients 21 ; furthermore, another study shows that miR-125a negatively correlates with disease severity in patients with Crohn's disease. 22 However, the correlation of lnc-NEAT1 with miR-21 and miR-125a, as well as their correlation with clinical characteristics in RA, is rarely reported.…”

“…Furthermore, we also found that lnc‐NEAT1 was negatively correlated with miR‐21 and miR‐125a, which might be explained by that lnc‐NEAT1 could target miR‐21 and miR‐125a in immunological disease (such as allergic rhinitis), the similar targeting manner might also exist in RA. 7 …”

“…Thus, we conducted the present study and found that in RA patients, increased lnc‐NEAT1 but declined miR‐21 and miR‐125a were correlated with inflammation and disease activity in RA patients. The possible reasons might be that: (a) elevated lnc‐NEAT1 and decreased miR‐21 and miR‐125a could promote inflammatory cytokines and immune response, which consequently resulted in elevated inflammation in RA patients 7 , 19 , 20 , 23 ; and (b) increased lnc‐NEAT1 and declined miR‐21 and miR‐125a might inhibit new bone formation and accelerate bone degradation through regulating several signaling pathways (such as Janus kinase 2/signal transducer, activator of transcription‐3, and E26 transformation‐specific‐1 pathways), which could lead to high disease activity in RA patients. 24 , 25 , 26 Therefore, elevated lnc‐NEAT1 but declined miR‐21 and miR‐125a were correlated with inflammation and disease activity in RA.…”

“…Long non‐coding RNA nuclear paraspeckle assembly transcript 1 (lnc‐NEAT1) is viewed to play an important role in regulating inflammation and autoimmunity. 7 , 8 , 9 , 10 , 11 , 12 For instance, it has been presented that lnc‐NEAT1 is able to target microRNA (miR)‐21 and miR‐125a to modulate inflammation 7 , 8 , 9 ; additionally, lnc‐NEAT1 promotes the proliferation of fibroblast‐like synoviocytes cells and production of inflammatory cytokines in RA via targeting miR‐204‐5p 10 ; meanwhile, lnc‐NEAT1 mediates inflammatory response in inflammatory bowel disease via regulating exosome‐mediated polarization of macrophages. 11 Furthermore, lnc‐NEAT1 is also engaged in autoimmune disease in the clinical field.…”

Long non‐coding RNA growth arrest‐specific 5 and its targets, microRNA‐21 and microRNA‐140, are potential biomarkers of allergic rhinitis

“…Furthermore, it is possible that miR-21 and miR-140 upregulate Th1 differentiation but downregulate Th2 differentiation by targeting GATA-3 and Tbet. 17,22,23 This would explain the positive correlation of miR-21 and miR-140 expression with Th1 cytokine expression as well as the negative correlation of miR-21 and miR-140 expression with Th2 cytokine expression.…”