The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism

Park, In Soo; Han, Min; Rhie, Jong Won; Kim, Soo Hyun; Jung, Youngmee; Kim, Ik Hwan; Kim, Sang Heon

doi:10.1016/j.biomaterials.2009.08.057

Cited by 56 publications

(49 citation statements)

References 33 publications

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“…A higher expression of fibronectin was seen in the EB group (see Fig. 1F), suggesting proliferation and differentiation in adipose cells, as previously described elsewhere (25,26).…”

supporting

confidence: 66%

Fat tissue morphology of long-term sex steroid deficiency and estrogen treatment in female rats

Carlos

Costa

Armada

et al. 2011

Fertility and Sterility

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“…A higher expression of fibronectin was seen in the EB group (see Fig. 1F), suggesting proliferation and differentiation in adipose cells, as previously described elsewhere (25,26).…”

supporting

confidence: 66%

Fat tissue morphology of long-term sex steroid deficiency and estrogen treatment in female rats

Carlos

Costa

Armada

et al. 2011

Fertility and Sterility

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“…It has been found that integrin β1 and αV mediated MSCs adhesion, migration and engraftment in myocardium [25], [26], [27]. The qRT-PCR analysis showed that the mRNA level of integrin β1 and αV in H 2 O 2 treated cells were significantly down-regulated compared with that in control ( p <0.05), whereas the reduced mRNA level of both integrins could be partially restored by Exendin-4 pretreatment (Fig.…”

Section: Resultsmentioning

confidence: 80%

Exendin-4 Pretreated Adipose Derived Stem Cells Are Resistant to Oxidative Stress and Improve Cardiac Performance via Enhanced Adhesion in the Infarcted Heart

Liu

Wang

et al. 2014

PLoS ONE

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Reactive oxygen species (ROS), which were largely generated after myocardial ischemia, severely impaired the adhesion and survival of transplanted stem cells. In this study, we aimed to determine whether Exendin-4 pretreatment could improve the adhesion and therapeutic efficacy of transplanted adipose derived stem cells (ADSCs) in ischemic myocardium. In vitro, H2O2 was used to provide ROS environments, in which ADSCs pretreated with Exendin-4 were incubated. ADSCs without pretreatment were used as control. Then, cell adhesion and viability were analyzed with time. Compared with control ADSCs, Exendin-4 treatment significantly increased the adhesion of ADSCs in ROS environment, while reduced intracellular ROS and cell injury as determined by dihydroethidium (DHE) staining live/Dead staining, lactate dehydrogenase-release assay and MTT assay. Western Blotting demonstrated that ROS significantly decreased the expression of adhesion-related integrins and integrin-related focal adhesion proteins, which were significantly reversed by Exendin-4 pretreatment and followed by decreases in caspase-3, indicating that Exendin-4 may facilitate cell survival through enhanced adhesion. In vivo, myocardial infarction (MI) was induced by the left anterior descending artery ligation in SD rats. Autologous ADSCs with or without Exendin-4 pretreatment were injected into the border area of infarcted hearts, respectively. Multi-techniques were used to assess the beneficial effects after transplantation. Longitudinal bioluminescence imaging and histological staining revealed that Exendin-4 pretreatment enhanced the survival and differentiation of engrafted ADSCs in ischemic myocardium, accompanied with significant benefits in cardiac function, matrix remodeling, and angiogenesis compared with non-pretreated ADSCs 4 weeks post-transplantation. In conclusion, transplantation of Exendin-4 pretreated ADSCs significantly improved cardiac performance and can be an innovative approach in the clinical perspective.

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“…Tissue was processed to isolate hASCs as described previously [23]. Isolated cells were cultured in high-glucose DMEM supplemented with 10% FBS and 1% antibiotics (penicillin/streptomycin).…”

Section: A N U S C R I P Tmentioning

confidence: 99%

Biomimetic 3D tissue printing for soft tissue regeneration

et al. 2015

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The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism

Cited by 56 publications

References 33 publications

Fat tissue morphology of long-term sex steroid deficiency and estrogen treatment in female rats

Fat tissue morphology of long-term sex steroid deficiency and estrogen treatment in female rats

Exendin-4 Pretreated Adipose Derived Stem Cells Are Resistant to Oxidative Stress and Improve Cardiac Performance via Enhanced Adhesion in the Infarcted Heart

Biomimetic 3D tissue printing for soft tissue regeneration

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