The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus

Wells, Rachel; Trick, Martin; Soumpourou, Eleni; Clissold, Leah; Morgan, Colin; Werner, P.; Gibbard, Carl; Clarke, Matthew; Jennaway, R.; Bancroft, Ian

doi:10.1007/s11032-013-9954-5

Cited by 54 publications

(56 citation statements)

References 32 publications

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“…Oil biosynthesis genes have undergone expansion in B. napus which exceeds that known in other oilseed plants (Chalhoub et al, 2014). Polyploidy and the genome complexity of B. napus limit translation of fundamental knowledge from A. thaliana into oilseed rape crop improvement as identification of individual genes controlling natural variation in this crop is challenging (Wells et al, 2014). …”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Study of Genetic Control of Seed Fatty Acid Biosynthesis in Brassica napus

et al. 2017

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Fatty acids and their composition in seeds determine oil value for nutritional or industrial purposes and also affect seed germination as well as seedling establishment. To better understand the genetic basis of seed fatty acid biosynthesis in oilseed rape (Brassica napus L.) we applied a genome-wide association study, using 91,205 single nucleotide polymorphisms (SNPs) characterized across a mapping population with high-resolution skim genotyping by sequencing (SkimGBS). We identified a cluster of loci on chromosome A05 associated with oleic and linoleic seed fatty acids. The delineated genomic region contained orthologs of the Arabidopsis thaliana genes known to play a role in regulation of seed fatty acid biosynthesis such as Fatty acyl-ACP thioesterase B (FATB) and Fatty Acid Desaturase (FAD5). This approach allowed us to identify potential functional genes regulating fatty acid composition in this important oil producing crop and demonstrates that this approach can be used as a powerful tool for dissecting complex traits for B. napus improvement programs.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Study of Genetic Control of Seed Fatty Acid Biosynthesis in Brassica napus

et al. 2017

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“…Our studies do, however, provides insights into limitations imposed by the availability of monounsaturated 18‐carbon moieties for elongation to VLCFAs. Recapitulation of the combination of Bna.fad2 mutations shown previously to greatly restrict the conversion of oleic acid to PUFAs (Wells et al , ), but, in a high erucic acid background, increases the availability of C18:1 for extension by the Brassica elongases (controlled by the Bna.FAE1 loci). The observed impact is to increase the proportion of erucic acid in seed oil (by ~17% of its original value), demonstrating that the availability of C18:1 is, indeed, an important limitation for erucic acid content.…”

Section: Discussionmentioning

confidence: 99%

“…We had been unable to amplify the third orthologue of FAD2 reported previously as being required in inactive form for manifestation of the low PUFA trait (Bna.-fad2.C1), and all individuals selected as inheriting low PUFA alleles Bna.fad2.A5 and Bna.fad2.C5 had inherited the low PUFA trait. From this, we deduce that variety Maplus already has the inactive (deleted) allele Bna.fad2.C1 as well as the inactive allele Bna.fad2.A1 that appears common in European rapeseed (Wells et al, 2014). Out of 192 F 2 plants, one HELP plant '2-91' from cross Maplus x K0472 (K0472-HE) and another HELP plant '4-87' from cross Maplus x K0047 (K0047-HE) were selected.…”

Section: Help Developmentmentioning

confidence: 99%

“…B. napus has four orthologues of FAD2 : Bna.FAD2.A1, Bna.FAD2.A5 , Bna.FAD2.C1 and Bna.FAD2.C5 , corresponding to BnaA.FAD2.b , BnaA.FAD2.a, BnaC.FAD2.b and BnaC.FAD2.a , respectively, in previous studies (Hu et al , ; Scheffler et al , ; Schierholt et al ., ; Smooker et al , ; Yang et al , ). A recent study (Wells et al , ) showed that, in rapeseed variety Cabriolet, only Bna.FAD2.C5 is functional. Thus, Bna.FAD2.C5 was targeted for chemical mutagenesis, resulting in population JBnaCAB_E, from which an allelic series of mutations of this gene was characterized.…”

Section: Introductionmentioning

confidence: 97%

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The impact of reducing fatty acid desaturation on the composition and thermal stability of rapeseed oil

Kaur

Wang

Stawniak

et al. 2019

Plant Biotechnology Journal

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Summary Oilseed rape (Brassica napus) is the third largest source of vegetable oil globally. In addition to food uses, there are industrial applications that exploit the ability of the species to accumulate the very‐long‐chain fatty acid (VLCFA) erucic acid in its seed oil, controlled by orthologues of FATTY ACID ELONGASE 1 (Bna.FAE1.A8 and Bna.FAE1.C3). The proportion of polyunsaturated fatty acids (PUFAs) in rapeseed oil is predicted to affect its thermal stability and is controlled by orthologues of FATTY ACID DESATURASE 2, particularly Bna.FAD2.C5. Our aim was to develop rapeseed lines combining high erucic and low PUFA characters and to assess the impact on thermal stability of the oil they produce. The new type of rapeseed oil (high erucic low polyunsaturate; HELP) contained a substantially greater proportion of erucic acid (54%) compared with high erucic rapeseed oil (46%). Although the total VLCFA content was greater in oil from HELP lines (64%) than from high erucic rapeseed (57%), analysis of triacylglycerol composition showed negligible incorporation of VLCFAs into the sn‐2 position. Rancimat analysis showed that the thermal stability of rapeseed oil was improved greatly as a consequence of reduction of PUFA content, from 3.8 and 4.2 h in conventional low erucic and high erucic rapeseed oils, respectively, to 11.3 and 16.4 h in high oleic low PUFA (HOLP) and HELP oils, respectively. Our results demonstrate that engineering of the lipid biosynthetic pathway of rapeseed, using traditional approaches, enables the production of renewable industrial oils with novel composition and properties.

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“…nutrisunoil.com/). An ethyl methanesulphonate mutagenised population was developed in Brassica napus (Wells et al, 2014) to study the effect of mutations on fatty acid desaturases. One of these mutants had 6% of polyunsaturated fatty acids while the oleic acid content reached 84%.…”

Section: Quality Traits: Diversification a Step Aheadmentioning

confidence: 99%

Research fields, challenges and opportunities in European oilseed crops breeding

Vincourt¹

2014

OCL

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-Due to the geographical specialization in oilseed world production, Europe has a major role to play in winter oilseed rape and sunflower breeding. Mainly based on the most recen t results, this review aims to identify the main research and breeding targets for these two crops, as seen through publications, with an attempt to suggest what are opportunities and challenges in these research fields. Growing a healthy and yielding crop remains the key driver for agronomic production. However sustainability and environmental profiles of the cultivar are now entering the field of play: The sustainability concern invested the field of resistance to diseases. Nitrogen use efficiency became an important target for Brassica napus, and crop resilience toward drought stresses is the way chosen in Helianthus annuus breeding for yield improvement. Significant advances are underway for quality traits, but the uncertainty on nutritional and industrial demand may explain why the product diversification remains low. Keywords:Brassica napus / Helianthus annuus / breeding target / biotic / abiotic Résumé -Champs de recherche, défis et opportunités de la sélection variétale des oléagineux européens. En raison de la spécialisation géographique dans la production mondiale d'oléagineux, l'Europe joue un rôle majeur dans la sélection de colza d'hiver et de tournesol. Reposant principalement sur les derniers résultats disponibles, cette revue cherche à identifier les principaux objectifs de recherche et de sélection de ces deux cultures, au regard des publications, et tente de suggérer quels sont les opportunités et défis de ces domaines de recherche. Faire croître des cultures saines et à haut rendement demeure un objectif clef de la production agronomique. Cependant la durabilité et les profils environnementaux de la variété cultivée entrent maintenant en ligne de compte : la préoccupation de durabilité a investi le champ de la résistance aux maladies. L'efficacité de l'utilisation de l'azote est devenu un objectif important pour Brassica napus, et la résistance des cultures au stress hydrique est l'une des voies majeures retenues pour la sélection chez Helianthus annuus. Des progrès significatifs sont en cours pour améliorer les traits qualitatifs, mais l'incertitude de la demande nutritionnelle et industrielle peut expliquer pourquoi la diversification de la production reste faible. Mots clés :Brassica napus / Helianthus annuus / sélection / facteur biotique / facteur abiotique

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The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus

Cited by 54 publications

References 32 publications

Genome-Wide Association Study of Genetic Control of Seed Fatty Acid Biosynthesis in Brassica napus

Genome-Wide Association Study of Genetic Control of Seed Fatty Acid Biosynthesis in Brassica napus

The impact of reducing fatty acid desaturation on the composition and thermal stability of rapeseed oil

Research fields, challenges and opportunities in European oilseed crops breeding

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