The Contrary Effects of Sirt1 on MCF7 Cells Depend on CD36 Expression Level

Yao, Yu; Liu, Tao; Wang, Xiaolan; Zhang, Dianlong

doi:10.1016/j.jss.2019.01.046

Cited by 6 publications

(5 citation statements)

References 34 publications

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“…The lack of protection is probably related to the active concentration of this antimicrobial peptide [ 24 ]. These results are consistent with those reported by Yao and Duan, who reported that high concentrations inhibited growth, but low concentrations promoted cell growth [ 25 26 ]. Therefore, a suitable concentration range must be determined.…”

Section: Discussionsupporting

confidence: 93%

Antiviral effects of Bovine antimicrobial peptide against TGEV in vivo and in vitro

Liang

Zhang²,

Lian³

et al. 2020

J Vet Sci

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Background: In suckling piglets, transmissible gastroenteritis virus (TGEV) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. This study explored methods of preventing or inhibiting their production. Bovine antimicrobial peptide-13 (APB-13) has antibacterial, antiviral, and immune functions. Objectives: This study analyzed the efficacy of APB-13 against TGEV through in vivo and in vitro experiments. Methods: The effects of APB-13 toxicity and virus inhibition rate on swine testicular (ST) cells were detected using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT). The impact of APB-13 on virus replication was examined through the 50% tissue culture infective dose (TCID 50). The mRNA and protein levels were investigated by real-time quantitative polymerase chain reaction and western blot (WB). Tissue sections were used to detect intestinal morphological development. Results: The safe and effective concentration range of APB-13 on ST cells ranged from 0 to 62.5 µg/mL, and the highest viral inhibitory rate of APB-13 was 74.1%. The log 10 TCID 50 of 62.5 µg/mL APB-13 was 3.63 lower than that of the virus control. The mRNA and protein expression at 62.5 µg/mL APB-13 was significantly lower than that of the virus control at 24 hpi. Piglets in the APB-13 group showed significantly lower viral shedding than that in the virus control group, and the pathological tissue sections of the jejunum morphology revealed significant differences between the groups. Conclusions: APB-13 exhibited good antiviral effects on TGEV in vivo and in vitro.

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Section: Discussionsupporting

confidence: 93%

Antiviral effects of Bovine antimicrobial peptide against TGEV in vivo and in vitro

Liang

Zhang²,

Lian³

et al. 2020

J Vet Sci

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“…Therefore, high levels of SIRT1 may cause cell cycle arrest. More interestingly, SIRT1 overexpression was found to inhibit cell proliferation in the MCF-7 cell line [59] , which is in good agreement with this model. In summary, when SIRT1 is overexpressed, lower DNA damage drives P53 periodic pulsing-regulated cell cycle arrest, and higher DNA damage drives P53 monotonic increase and apoptosis.…”

Section: Resultssupporting

confidence: 84%

Dual roles of SIRT1 in the BAX switch through the P53 module: A mathematical modeling study

Liu

Yang

2021

Computational and Structural Biotechnology Journal

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“…It also interacts with transcription factors or co-factors, including p53, FOXO family member, NF-κB, myoblast determination protein 1, nuclear receptor corepressor, p300 and peroxisome proliferator-activated receptor γ coactivator 1-α, and regulates their transcriptional activity. SIRT1 inhibitors exhibit antitumor activity, which has been observed in cells from breast, thyroid, lung, pancreatic, prostate, hepatitis and colon cancer (17)(18)(19)(20)(21). Moreover, SIRT1 has effective antitumor activity against hematological malignancies, such as T/B-cell lymphoma, chronic myeloid leukemia (CML), leukemia and other hematological malignancies (11,(22)(23)(24)(25)(26).…”

Section: Discussionmentioning

confidence: 99%

Targeting SIRT1 to inhibit the proliferation of multiple myeloma cells

et al. 2021

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Multiple myeloma (MM) is the second most common hematopoietic malignancy and remains an incurable disease. Thus, novel drugs and therapeutic methods are required for patients with MM. The present study aimed to investigate the effect of sirtuin 1 (SIRT1) inhibitor cambinol on the proliferation and apoptosis of myeloma cell lines, RPMI8226 and U266. Moreover, the present study evaluated the underlying molecular mechanisms of proliferation inhibition and apoptosis induced by cambinol. A Cell Counting Kit-8 assay was used to measure the viability of RPMI8226 and U266 cells treated with cambinol. Apoptosis and the cell cycle were analyzed via flow cytometry. The expression levels of caspase-3, poly(ADP-ribose) polymerase 1 (PARP), p53, acetylated p53 (Ac-p53), Bcl-2, cyclin D1 and p21 were detected in cells treated with cambinol using western blot analysis. The results demonstrated that cambinol inhibited the proliferation of RPMI8226 and U266 cells in a time- and dose-dependent manner. Increased apoptosis and G 1 cell cycle arrest, together with enhanced procaspase-3 degradation and PARP cleavage were identified in cambinol-treated cells compared with controls. Western blotting results also revealed the upregulation of p53 acetylation and p21, as well as the downregulation of Bcl-2 and cyclin D1 in cells treated with cambinol. In conclusion, the present results suggest that cambinol inhibits the proliferation and induces apoptosis in RPMI8226 and U266 cells by regulating acetylation of p53 via the targeting of SIRT1.

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The Contrary Effects of Sirt1 on MCF7 Cells Depend on CD36 Expression Level

Cited by 6 publications

References 34 publications

Antiviral effects of Bovine antimicrobial peptide against TGEV in vivo and in vitro

Antiviral effects of Bovine antimicrobial peptide against TGEV in vivo and in vitro

Dual roles of SIRT1 in the BAX switch through the P53 module: A mathematical modeling study

Targeting SIRT1 to inhibit the proliferation of multiple myeloma cells

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