The contractile effects of several substituted short analogues of porcine galanin in isolated rat jejunal and colonic smooth muscle strips

Umer, Artur; Ługowska, H.; Anand, Jacek Sein; Rekowski, Piotr; Ruczyński, Jarosław; Petrusewicz, Jacek; Korolkiewicz, Roman

doi:10.1016/j.phrs.2005.05.001

Cited by 8 publications

(8 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is unlikely that galanin evoked inhibition to be attributable to a non‐specific action of galanin on our preparation as galantide, a non selective galanin receptor antagonist, 32–34 could significantly block the inhibitory effect of galanin. In some experimental preparations using other portions of the gastrointestinal tract, galanin had a direct effect mediated by galanin receptor expressed on smooth muscle fibres 13,19 . The inability of galanin to influence the electrical field stimulation induced contractions in the present experiments excluded a direct muscular effect and suggests an indirect neuronal pathway for its inhibitory action.…”

Section: Discussionmentioning

confidence: 43%

“…Galanin, a 29‐amino acid neuropeptide originally isolated from porcine gut, 10 is widely distributed in both central and peripheral nervous systems 3,11,12 . In the gastrointestinal tract, galanin modulates transmitter release, secretion and motility by inhibiting neurogenic contractions and acetylcholine release 13–19 . Effects of galanin are mediated by three distinct G‐protein coupled receptors: galanin receptor 1 (G i/o ), galanin receptor 2 (G q/11 ) and galanin receptor 3 (G i/o ) 20–23 …”

Section: Introductionmentioning

confidence: 99%

“…3,11,12 In the gastrointestinal tract, galanin modulates transmitter release, secretion and motility by inhibiting neurogenic contractions and acetylcholine release. [13][14][15][16][17][18][19] Effects of galanin are mediated by three distinct G-protein coupled receptors: galanin receptor 1 (G i/o ), galanin receptor 2 (G q/11 ) and galanin receptor 3 (G i/o ). [20][21][22][23] The purpose of the present study was to determine any role of galanin in modulating vagally induced contractions of striated oesophageal muscle and to test the hypothesis that galanin mediates the TRPV1-/NOindependent effect of piperine.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Galanin modulates vagally induced contractions in the mouse oesophagus

Boudaka

Wörl

Shiina

et al. 2009

Neurogastroenterology Motil

View full text Add to dashboard Cite

Nitrergic myenteric neurons co-innervating motor endplates were previously shown to inhibit vagally induced contractions of striated muscle in the rodent oesophagus. Immunohistochemical demonstration of putative co-transmitters, e.g. galanin, in enteric neurons prompted us to study a possible role of galanin in modulating vagally mediated contractions in an in vitro vagus nerve-oesophagus preparation of the mouse. Galanin (1-16) (1-100 nmol L(-1)), in the presence of the peptidase inhibitor, phenanthroline monohydrate, inhibited vagally induced contractions in a concentration-dependent manner (control: 100%; galanin 1 nmol L(-1): 95.6 +/- 1.6%; galanin 10 nmol L(-1): 57.3 +/- 6.5%; galanin 100 nmol L(-1): 31.2 +/- 8.1%, n = 5). The non-selective galanin receptor antagonist, galantide (100 nmol L(-1)), blocked the inhibitory effect of galanin (10 nmol L(-1)) while the selective non-galanin receptor 1 and galanin receptor 3 antagonists, M871 (1 micromol L(-1)) and SNAP37889 (100 nmol L(-1)), respectively, and the nitric oxide synthase inhibitor, NG-nitro-l-arginine methyl ester (L-NAME) (200 micromol L(-1)), failed to affect this galanin-induced response. Simultaneous application of galantide (100 nmol L(-1)) and L-NAME (200 micromol L(-1)) significantly reduced the inhibitory effect of capsaicin (30 mumol L(-1)) on vagally induced contractions when compared with its effect in the presence of L-NAME alone or in combination with the selective galanin receptor 2 or 3 antagonists. An inhibitory effect of piperine on vagally induced contractions was reduced neither by galantide nor by L-NAME. Immunohistochemistry revealed galanin immunoreactive myenteric neurons and nerve fibres intermingling with cholinergic vagal terminals at motor endplates. These data suggest that galanin from co-innervating enteric neurons co-operates with nitric oxide in modulating vagally induced contractions in the mouse oesophagus.

show abstract

Section: Discussionmentioning

confidence: 43%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Galanin modulates vagally induced contractions in the mouse oesophagus

Boudaka

Wörl

Shiina

et al. 2009

Neurogastroenterology Motil

View full text Add to dashboard Cite

show abstract

“…Within GI tract GAL has been involved in the regulation of blood flow, water and electrolytes transport and gut secretion (Furness et al, 1987;FoxThrelkeld et al, 1991;Piqueras et al, 2005). Furthermore, GAL has affected gut motility both by action on the intestinal smooth muscle cells (Umer et al, 2005) and by indirect neuromodulatory mechanisms such as neurotransmitter release (Sarnelli et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

Proliferative enteropathy (PE): Induced changes in galanin-like immunoreactivity in the enteric nervous system of the porcine distal colon

Gonkowski¹,

Burliński²,

Całka³

2009

Acta vet. (Beogr.)

View full text Add to dashboard Cite

The aim of this study was to investigate the changes of galanin (GAL) like immonoreactivity in the porcine descending colon during proliferative entheropathy (PE). Accordingly, the distribution pattern of GAL - like immunoreactive (GAL-LI) nerve structures was studied by the immunofluorescence technique in the circular muscle layer, myenteric (MP), outer submucous (OSP) and inner submucous plexuses (ISP), as well as in the mucosal layer of the porcine descending colon under physiological conditions and during PE. In control animals GAL-LI perikarya have been shown to constitute 4.03 ± 0.1%, 6.67 ± 0.3% and 11.20 ± 0.5% in MP, OSP and ISP, respectively. PE caused changes in the GAL - like immunoreactivity, which differed in particular parts of the studied bowel segment. During PE the number of GAL-LI perikarya amounted to 2.90±0.5%, 8.42±1.0% and 21.72±1,4% within the MP, OSP and ISP, respectively. Moreover PE caused an increase in the number of GAL-LI nerve fibers in the colonic circular muscle and mucosal layers, as well as in all intramural plexuses, especially in ISP, where nearly every ganglion contained a very dense meshwork of the GAL-positive nerve fibers under the studied pathological factor. This study for the first time reports on changes in GAL-LI nerve structures of the porcine descending colon during Lawsonia intracellularis infection

show abstract

“…Small intestinal preparations were prepared according to the methods described previously with slight modification [25][26][27] .…”

Section: Tissue Preparation and Intestinal Contractility Determinationmentioning

confidence: 99%

Hesperidin alleviates rat postoperative ileus through anti-inflammation and stimulation of Ca2+-dependent myosin phosphorylation

et al. 2016

View full text Add to dashboard Cite

show abstract

The contractile effects of several substituted short analogues of porcine galanin in isolated rat jejunal and colonic smooth muscle strips

Cited by 8 publications

References 20 publications

Galanin modulates vagally induced contractions in the mouse oesophagus

Galanin modulates vagally induced contractions in the mouse oesophagus

Proliferative enteropathy (PE): Induced changes in galanin-like immunoreactivity in the enteric nervous system of the porcine distal colon

Hesperidin alleviates rat postoperative ileus through anti-inflammation and stimulation of Ca2+-dependent myosin phosphorylation

Contact Info

Product

Resources

About