The Content of Phenols (Including Flavonoids) and Antioxidants in the Leaves of Viscum Album L. And Pyrus Communis L

Adzhiahmetova, Similla; Chervonnaja, N.M.; Pozdnjakov, D.I.; Oganesjan, E.T.

doi:10.29296/25877313-2021-02-03

Cited by 4 publications

(1 citation statement)

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“…(2021) продемонстрировали, что регулярное употребление богатых полифенолами экстрактов или отдельных фенольных соединений улучшает функцию эндотелия при различных патологиях, включая интоксикацию этанолом [14]. В ранее проведенных исследованиях было установлено, что водно-этанольное извлечение из листьев омелы белой содержит более 13% веществ полифенольной структуры и оказывает выраженное антиоксидантное действие [15]. В этой связи экстракт листьев омелы белой был выбран в качестве исследуемого объекта для данного исследования.…”

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The effect of dry extract of white mistletoe leaves on the change of vasodilation function of the cerebral vascular endothelium in rats with chronic alcohol intoxication

Pozdnyakov,

Vihor',

Adjiakhmetova

2023

Science and Innovations in Medicine

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Aim to evaluate the effect of dry extract of mistletoe leafs on the change of vascular endothelium vasodilation function in rats under conditions of chronic alcohol intoxication. Material and methods. The chronic alcohol intoxication was modeled in female Wistar rats by course of oral administration of ethanol at the rate of 3g/kg/day. The tested extract and the reference drug ethylmethylhydroxypyridine succinate were administered per os at a dose of 100 mg/kg. The vasodilation function of the vascular endothelium was assessed by the change in the rate of cerebral blood flow during modification of the endogenous nitric oxide synthesis. The concentration of active forms of endothelial and inducible nitric oxide synthase isofrom was determined by enzyme immunoassay. Results. The course of the tested extract and the reference drug resulted in an increase in the rate of cerebral blood flow in relation to untreated animals by 48.1% (p0.05) and 40.2% (p0.05), respectively. The restoration of endothelium-dependent vasodilation of cerebral vessels was also noted with the administration of the studied extract of white mistletoe and the reference drug. The analysis of changes in the NO-synthase system showed that, when using the tested extract, the concentration of eNOS increased by 76.3% (p0.05), and iNOS, on the contrary, decreased by 22.1% (p0.05), which was comparable with the indicators of the group of animals receiving the reference drug. Conclusion. The use of mistletoe leaf dry extract leads to the restoration of endothelium-dependent vasodilation of cerebral vessels in rats with chronic alcohol intoxication, probably due to the improvement of eNOS-dependent mechanisms of nitric oxide synthesis.

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Section: Discussionunclassified

The effect of dry extract of white mistletoe leaves on the change of vasodilation function of the cerebral vascular endothelium in rats with chronic alcohol intoxication

Pozdnyakov,

Vihor',

Adjiakhmetova

2023

Science and Innovations in Medicine

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Interrelation Between Mitochondrial Enzyme Activity and Antioxidant Activity of Secondary Polyphenol Nature Metabolites in Hemiparasite Viscum Album L. Leaves

Adzhiakhmetova

Поздняков

E.O.

et al. 2022

Farm. farmakol. (Pâtigorsk)

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Antioxidants are widely used in practical medicine. Not only the search for new plant antioxidants, but also the study of the factors affecting their accumulation in plants, are relevant.The aim is to study the interrelation between the activity of mitochondrial enzymes and the antioxidant activity of the secondary polyphenolic nature metabolites in hemiparasite Viscum Album L. leaves.Materials and methods. The sampling material was Viscum album L. leaves, collected in winter from a host tree, Malus domestica Borkh. The extraction of biologically active substances was carried out with ethyl alcohol of various concentrations (90%, 70% and 50%), or purified water. The amount of total antioxidants was estimated by the amperometric method. The amount of total phenols was evaluated in the reaction with the Folin-Ciocalteu reagent. The concentration of total flavonoids was estimated by the change in the optical density of the rutin with aluminum (III) chloride complex. The antioxidant properties of the analyzed extracts were determined in vitro in the induced lipid peroxidation test. The activity of aconitase was assessed by a conjugated aconitase-isocitrate dehydrogenase reaction, citrate synthase – by changing the color intensity of the Ellman reagent solution, succinate dehydrogenase were determined in the reaction of succinate-dependent oxidation of 2,6-dichlorophenolindophenol.Results. The carried out study showed that the maximum amount of total phenols (2.39±0.05%) is observed in a 50% ethanol extract from Viscum album L. leaves, with the content of total flavonoids equal to 1.83±0.04%, and the antioxidants equal to 0.503±0.007 mg/g (a quercetin equivalent) and 0.322±0.006 mg/g (a gallic acid equivalent). A 50% ethanol extract suppressed lipid peroxidation in the model mixture with IC50=106.3±1.09 µg/ml. In Viscum album L. leaves, a high activity of aconitase which strongly correlated (r=0.88416) with changes in the concentration of flavonoids, has been notified.Conclusion. The optimal extractant for obtaining extracts with a high antioxidant activity is 50% ethyl alcohol. The analysis of the mitochondrial enzymes activity showed that in Viscum album L. leaves collected in winter, a high activity of aconitase strongly correlated with changes in the concentration of flavonoids (r=0.88416).

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The Choice of Technology for Obtaining an Extemporal Dosage Form From Raw Black Currant (Ribes Nigri Cormus)

O.V.,

V.D.,

I.V.

2024

Mediko-farmacevtičeskij žurnal "Pulʹs"

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When studying a new type of raw material, a rational choice of dosage form plays an important role in order to obtain a pronounced pharmacological effect. Black currant (Ribes nigrum L.) belongs to the family Grossulariaceae – gooseberries. This is one of the most common berry crops both in industrial gardens and in private plots. Black currant shoots, as easily accessible raw materials, are of interest for study in order to establish the possibility of their use in scientific medicine. The article presents the results of the choice of technology for obtaining an extemporal dosage form from black currant shoots. The content of biologically active substances (BAS) in raw materials – black currant shoots, as well as the yield of BAS: flavonoids, polysaccharides, tannins and ascorbic acid in the infusion and decoction of currant shoots was investigated. The content of flavonoids was determined by differential spectrophotometry on the SF-2000 spectrophotometer, polysaccharides by gravimetric method, tannins and ascorbic acid by titrimetric method using pharmacopoeial techniques. The content of BAS in the raw materials – black currant shoots: for flavonoids - 2.64%, polysaccharides – 27.85%, tannins – 8.98% and ascorbic acid – 0.8%; in the infusion: flavonoids – 1.98%, polysaccharides – 25.67%, tannins – 7.89%, ascorbic acid – 0.6%; and in decoction of currant shoots: flavonoids – 1.65%, polysaccharides – 23.88%, tannins – 8.89%, ascorbic acid – 0.4%. Based on the conducted research, it was concluded that the infusion is the optimal extemporal medicinal form of black currant shoots, since it contains the largest amount of biologically active substances, and is promising for further study for use in medical practice.

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The Content of Phenols (Including Flavonoids) and Antioxidants in the Leaves of Viscum Album L. And Pyrus Communis L

Cited by 4 publications

References 0 publications

The effect of dry extract of white mistletoe leaves on the change of vasodilation function of the cerebral vascular endothelium in rats with chronic alcohol intoxication

The effect of dry extract of white mistletoe leaves on the change of vasodilation function of the cerebral vascular endothelium in rats with chronic alcohol intoxication

Interrelation Between Mitochondrial Enzyme Activity and Antioxidant Activity of Secondary Polyphenol Nature Metabolites in Hemiparasite Viscum Album L. Leaves

The Choice of Technology for Obtaining an Extemporal Dosage Form From Raw Black Currant (Ribes Nigri Cormus)

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