The content of gonadotropin-releasing hormone (GnRH), kisspeptin, and estrogen receptors (ERα/ERβ) in the anteromedial hypothalamus displays daily variations throughout the rat estrous cycle

Olvera-Juárez, Esteban; Silva, Carlos-Camilo; Flores, Angélica; Arrieta‐Cruz, Isabel; Mendoza-Garcés, Luciano; Martínez-Coria, Hilda; López-Valdés, Héctor E.; Cárdenas, Mario; Domı́nguez, Roberto; Gutiérrez‐Juárez, Roger; Cruz, Maria Antonieta

doi:10.1007/s00441-020-03258-x

Cited by 6 publications

(6 citation statements)

References 48 publications

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“…Role of the Hypothalamus in the HPO Axis. Gene expression of estrogen-related receptors and proteins was quanti ed as the observed differences in circulating E 2 could modulate their expression via negative feedback [41,42].…”

Section: Resultsmentioning

confidence: 99%

“…We observed few hypothalamic gene differences among intact mice, within-group variability of circulating E 2 concentrations and estrous stage at the time of tissue collection may explain, in part, the lack of distinct differences in hypothalamic gene expression of HPO axis-related and estrogen receptor genes. Indeed, receptor (ERα, KISS1R) and molecule (GnRH, KISS1) expression uctuates throughout the human menstrual and rodent estrous cycles in response to circulating E 2 variations [41,42,55]. Further, the markers of attenuated E 2 observed in intact mice may not have persisted long enough to impact downstream hypothalamic gene expression [56].…”

Section: Discussionmentioning

confidence: 99%

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Ovarian status modulates endocrine and neuroinflammatory responses to a murine mammary tumor

Strehle

Russart

Burch

et al. 2022

American Journal of Physiology-Regulatory, Integrative and Comp

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Breast cancer patients have increased circulating inflammatory markers and mammary tumors increase neuroinflammation in rodent models. Menopausal status is not only important in the context of breast cancer as circulating estrogen influences tumor progression, but also because estrogen is anti-inflammatory and an essential modulator of endocrine function in the brain and body. Here, we manipulated "menopause" status (ovary-intact and ovariectomized) in an ER+ mouse mammary tumor model to determine the extent to which ovarian status modulates: 1) tumor effects on estrogen concentrations and signaling in the brain, 2) tumor effects on estrogen-associated neurobiology and inflammation, and 3) the ability for tumor resection to resolve the effects of a tumor. We hypothesized that reduced circulating estradiol (E2) following ovariectomy exacerbates tumor-induced peripheral and central inflammation. Notably, we observed ovarian-dependent modulation on tumor-induced peripheral outcomes, including E2-dependent processes and, to a lesser degree, circulating inflammatory markers. In the brain, ovariectomy exacerbated neuroinflammatory markers in select brain regions and modulated E2-related neurobiology due to a tumor and/or resection. Overall, our data suggest that ovarian status has moderate implications for tumor-induced alterations in neuroendocrinology and neuroinflammation and mild effects on peripheral inflammatory outcomes in this murine mammary tumor model.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ovarian status modulates endocrine and neuroinflammatory responses to a murine mammary tumor

Strehle

Russart

Burch

et al. 2022

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Considering that IGFBP-5 administration downregulated hypothalamic Gnrh gene expression levels, and the suppressed expression of GnRH was associated with decreased circulating levels of LH and FSH [ 33 ]. The suppressed circulating levels of these two hormones following IGFBP-5 injection were not surprising [ 55 ]. Puberty establishment is characterized by a progressive increase in the secretion of gonadotropic hormones that leads to an elevation in blood estradiol levels.…”

Section: Discussionmentioning

confidence: 99%

The expression of IGFBP-5 in the reproductive axis and effect on the onset of puberty in female rats

Yao

Lin

et al. 2022

Reprod Biol Endocrinol

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Insulin-like growth factor-binding protein-5 (IGFBP-5) has recently been shown to alter the reproductive capacity by regulating insulin-like growth factor (IGF) bioavailability or IGF-independent effects. The present study aimed to investigate the effect and mechanism of IGFBP-5 on the onset of puberty in female rats. Immunofluorescence and real-time quantitative PCR were used to determine the expression and location of IGFBP-5 mRNA and protein distribution in the infant's hypothalamus-pituitary-ovary (HPO) axis prepuberty, peripuberty, puberty and adult female rats. Prepubertal rats with IGFBP-5 intracerebroventricular (ICV) were injected to determine the puberty-related genes expression and the concentrations of reproductive hormones. Primary hypothalamic cells were treated with IGFBP-5 to determine the expression of puberty-related genes and the Akt and mTOR proteins. Results showed that Igfbp-5 mRNA and protein were present on the HPO axis. The addition of IGFBP-5 to primary hypothalamic cells inhibited the expression of Gnrh and Igf-1 mRNAs (P < 0.05) and increased the expression of AKT and mTOR protein (P < 0.01). IGFBP-5 ICV-injection delayed the onset of puberty, reduced Gnrh, Igf-1, and Fshβ mRNAs, and decreased the concentrations of E2, P4, FSH,serum LH levels and the ovaries weight (P < 0.05). More corpus luteum and fewer primary follicles were found after IGFBP-5 injection (P < 0.05).

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“…For example, within the rodent hypothalamus, both ERα and ERβ vary in concentration across the oestrous cycle. [45][46][47] Oestradiol supplementation increases Esr2 expression in the arcuate nucleus but decreases it in the paraventricular nucleus, amygdala, entorhinal cortex and thalamus. 33,34,48 Some studies show that oestradiol supplementation decreases Esr2 expression in the bed nucleus of the stria terminalis, 49,50 whereas others show no changes, 34 indicating that the effects of oestradiol on Esr2 levels are not always consistent.…”

Section: Discussionmentioning

confidence: 99%

Impact of pubertal onset on region‐specific Esr2 expression

Drzewiecki

Sellinger

Juraska

2021

J Neuroendocrinology

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The content of gonadotropin-releasing hormone (GnRH), kisspeptin, and estrogen receptors (ERα/ERβ) in the anteromedial hypothalamus displays daily variations throughout the rat estrous cycle

Cited by 6 publications

References 48 publications

Ovarian status modulates endocrine and neuroinflammatory responses to a murine mammary tumor

Ovarian status modulates endocrine and neuroinflammatory responses to a murine mammary tumor

The expression of IGFBP-5 in the reproductive axis and effect on the onset of puberty in female rats

Impact of pubertal onset on region‐specific Esr2 expression

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