The construction of transgenic &lt;i&gt;Forsythia&lt;/i&gt; plants: comparative study of three &lt;i&gt;Forsythia&lt;/i&gt; species

Morimoto, Kinuyo; Ono, Eiichiro; Kim, Hyun-Jung; Okazawa, Atsushi; Kobayashi, Akio; Satake, Honoo

doi:10.5511/plantbiotechnology.11.0125a

Cited by 10 publications

(6 citation statements)

References 27 publications

(28 reference statements)

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“…(Morimoto et al 2011), leafy shoots of Cryptomeria japonica (Ishii et al 2011), shoot explants of Anacardium occidentale (Thimmappaiah et al 2002) and Eucalyptus (Hajari et al 2006), and radicles of the olive (Cerezo et al 2011), as well as somatic embryogenesis of Olea europea (Shibli et al 2001). In this study, direct adventitious shoot organogenesis and plant regeneration were first established in N. cadamba using cotyledons as explants.…”

Section: Discussionmentioning

confidence: 99%

Direct adventitious shoot organogenesis and plant regeneration from cotyledon explants in Neolamarckia cadamba

Huang

Ouyang

et al. 2014

Plant Biotechnology

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Neolamarckia cadamba is a fast-growing and deciduous tropical hardwood with anatomical, morphological, and chemical characteristics that make it suitable for building materials, pulp production, and medicine raw materials. In this study, a protocol for direct adventitious shoot organogenesis and plant regeneration from the aseptic cotyledons of N. cadamba was established. The cotyledons with petioles from 3-week-old seedlings were used for adventitious shoot induction in DCR medium containing 22.20 µM 6-benzyladenine (BA) and 0.27 µM α-naphthaleneacetic acid (NAA). The frequency of adventitious shoot induction was 54.2%. Micro-shoots were then transferred to MS medium containing 4.44 µM BA and 0.25 µM indole-3-butyric acid (IBA) for shoot propagation. Available shoots per explant reached 5.9. The highest rooting percentage (98.3%) was obtained when shoots were transferred to half-strength MS medium supplemented with 0.27 µM NAA and 0.25 µM IBA. The rooted plantlets could be successfully acclimatized to a greenhouse with more than 95% survival, and the regenerated plants showed the same morphological characteristics as those of the control plants in fields. Histological observations revealed that the adventitious shoots only originated from the epidermal tissue around the edge of the cut zone of the cotyledonary petiole.

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Section: Discussionmentioning

confidence: 99%

Direct adventitious shoot organogenesis and plant regeneration from cotyledon explants in Neolamarckia cadamba

Huang

Ouyang

et al. 2014

Plant Biotechnology

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“…For instance, sesame seeds contain 100 times more sesamin than transgenic cells of Forsythia ; however, transgenic cells multiplied 10-times in two weeks [ 433 ], and they can be cultivated without seasonal interruptions. External factors such as light can be optimized in order to maximize sesamin production in cell cultures [ 447 , 448 ]. Culturing conditions exert tremendous effect on the metabolic productivity of tissues and cells.…”

Section: Outlook: Biotechnology In Lignan Productionmentioning

confidence: 99%

Lignans of Sesame (Sesamum indicum L.): A Comprehensive Review

et al. 2021

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Major lignans of sesame sesamin and sesamolin are benzodioxol--substituted furofurans. Sesamol, sesaminol, its epimers, and episesamin are transformation products found in processed products. Synthetic routes to all lignans are known but only sesamol is synthesized industrially. Biosynthesis of furofuran lignans begins with the dimerization of coniferyl alcohol, followed by the formation of dioxoles, oxidation, and glycosylation. Most genes of the lignan pathway in sesame have been identified but the inheritance of lignan content is poorly understood. Health-promoting properties make lignans attractive components of functional food. Lignans enhance the efficiency of insecticides and possess antifeedant activity, but their biological function in plants remains hypothetical. In this work, extensive literature including historical texts is reviewed, controversial issues are critically examined, and errors perpetuated in literature are corrected. The following aspects are covered: chemical properties and transformations of lignans; analysis, purification, and total synthesis; occurrence in Seseamum indicum and related plants; biosynthesis and genetics; biological activities; health-promoting properties; and biological functions. Finally, the improvement of lignan content in sesame seeds by breeding and biotechnology and the potential of hairy roots for manufacturing lignans in vitro are outlined.

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“…Forsythia plants have much greater biomass, with higher amount of lignans, than suspension cell cultures, and these plants can grow and propagate from small explants without flowering or seed formation. However, efficient generation of transgenic Forsythia plants still requires further basic research due to the markedly low transformation efficiency by any known gene transfection methods and deviation among Forsythia species [ 85 , 86 , 87 ]. In contrast, the generation of both stable (namely transgenic) and transient transfectants of Linum species are well established, and thus, the amounts of precursors or intermediates of targeted lignans are major determinants for the employment of cell cultures, organ cultures, or plants as host platforms.…”

Section: Metabolic Engineering Of Lignan Biosynthesismentioning

confidence: 99%

Essences in Metabolic Engineering of Lignan Biosynthesis

et al. 2015

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Lignans are structurally and functionally diverse phytochemicals biosynthesized in diverse plant species and have received wide attentions as leading compounds of novel drugs for tumor treatment and healthy diets to reduce of the risks of lifestyle-related non-communicable diseases. However, the lineage-specific distribution and the low-amount of production in natural plants, some of which are endangered species, hinder the efficient and stable production of beneficial lignans. Accordingly, the development of new procedures for lignan production is of keen interest. Recent marked advances in the molecular and functional characterization of lignan biosynthetic enzymes and endogenous and exogenous factors for lignan biosynthesis have suggested new methods for the metabolic engineering of lignan biosynthesis cascades leading to the efficient, sustainable, and stable lignan production in plants, including plant cell/organ cultures. Optimization of light conditions, utilization of a wide range of elicitor treatments, and construction of transiently gene-transfected or transgenic lignan-biosynthesizing plants are mainly being attempted. This review will present the basic and latest knowledge regarding metabolic engineering of lignans based on their biosynthetic pathways and biological activities, and the perspectives in lignan production via metabolic engineering.

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The construction of transgenic <i>Forsythia</i> plants: comparative study of three <i>Forsythia</i> species

Cited by 10 publications

References 27 publications

Direct adventitious shoot organogenesis and plant regeneration from cotyledon explants in Neolamarckia cadamba

Direct adventitious shoot organogenesis and plant regeneration from cotyledon explants in Neolamarckia cadamba

Lignans of Sesame (Sesamum indicum L.): A Comprehensive Review

Essences in Metabolic Engineering of Lignan Biosynthesis

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