The Concept of a Preniche for Localization of Future Metastases

Перельмутер, В. М.; Manskikh, V. N.

doi:10.1007/978-94-007-7602-9_11

Cited by 3 publications

(2 citation statements)

References 34 publications

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“…The increase of CTCs might be evidence of preponderance of intravasation over intravascular death or extravasation, while the reduce of CTCs might be indicator of predominance extravasation over intravasation. It should be taken into account that, for haeamatogenous metastases development, the most important property of “cells-seeds” is the ability to effectively interact with cell elements and molecules of premetastatic niches [ 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity of Circulating Tumor Cells in Neoadjuvant Chemotherapy of Breast Cancer

et al. 2018

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The biological properties of circulating tumor cells (CTCs), and their dynamics during neoadjuvant chemotherapy are important, both for disease progression prediction and therapeutic target determination, with the aim of preventing disease progression. The aim of our study was to estimate of different CTC subsets in breast cancer during the NACT (neoadjuvant chemotherapy). The prospective study includes 27 patients with invasive breast cancer, T2-4N0-3M0, aged 32 to 60 years. Venous heparinized blood samples, taken before and after biopsy, after each courses of chemotherapy (on days 3–7), and before surgical intervention, served as the material for this study. Different subsets of circulating tumor cells were determined on the basis of the expression of EpCAM, CD45, CD44, CD24, and N-Cadherin using flow cytometry. As the result of this study, it has been observed that significant changes in the quantity of the different subsets of circulating tumor cells in patients’ blood were observed after carrying out the 3rd course of NACT. NACT causes significant changes in the quantity of six CTC subsets, with various combinations of stemness and epithelial-mesenchymal transition (EMT) properties.

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Section: Discussionmentioning

confidence: 99%

Heterogeneity of Circulating Tumor Cells in Neoadjuvant Chemotherapy of Breast Cancer

et al. 2018

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“…Последние исследования показывают, что ПК КМ , экспрессирующие сосудистый фактор роста (VEGF)-R1 и VLA-4, под влиянием опухолевых экзосом создают в органереципиенте среду, богатую фибронектином, за счет секретирования матриксной металлопротеиназы-9, облегчающую метастазирование [24]. Также опухолевые экзосомы активно «вербуют» макрофаги, заставляя их экспрессировать провоспалительные маркеры интерлейкин-6, фактор некроза опухоли α, G-CSF и CCL2 за счет активации сигнального пути NF-κB [25], поскольку наличие хронического воспаления считается необходимым изменением в тканях органов-реципиентов [26,27]. Они обнаруживаются и в первичной опухоли, и в преметастатической, и метастатических нишах и регулируют процесс метастазирования сразу в нескольких направлениях [28], а ингибирование макрофагов приводит к подавлению процесса метастазирования [29].…”

Section: подготовка к формированию преметастатической нишиunclassified

Principles of premetastatic niche formation

et al. 2020

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The article is devoted to premetastatic niche as a complex term, including stromal cells, vessels, extracellular matrix and their changes during interaction with the primary tumor. On example of different malignant tumors authors describe as primary tumor through tumor exosomes prepares certain organs-recipients to metastatic clone implantation. In the area of premetastatic niche under the influence of tumorous exosomes polarization of macrophages towards M2 type takes place. The cells are the main agents, providing survival as well as migration of tumorous cells. Affecting extracellular matrix, macrophages change the microcirculatory bed permeability. This mechanism is directed towards increase of its permeability to entrance of metastatic clone cells form vessels into premetastatic niche. Besides macrophages fibroblasts and polypotent bone marrow stem cells are also reprogrammed, that results in metabolism and local immunity changes at the place of future implantation. As a result, only when tissue of recipient-organ is prepared for contact with metastatic clone, their interaction take place with consequent formation of secondary tumor metastatic niche. Thus, this review describes pathogenesis of metastasis, different from its early understanding as spread of metastatic clone with lymph and blood. These peculiarities may in future have significant impact in practical medicine, Blockage of signal spread from primary tumor through exosomes is one of the promising directions in pathogenetic therapy of malignant tumors. Investigation of principles of premetastatic niche formation may become a theoretical substantiation for prophylaxis of metastatic disease and inhibition of micrometastasis to macrometastasis transformation.

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Heterogeneity of premetastatic niches gene expression in breast cancer cells

et al. 2015

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To investigate the expression of the TGFB1, TNF, CSF1, CSF2, VEGFA and HIF1A genes in the patients with invasive breast carcinoma of no special type considering the intratumoral morphological heterogeneity. Methods. The technology of laser capture microdissection PALM was used to isolate five types of morphological tumor structures from three patients with invasive carcinoma of no special type (IC NST), luminal A subtype, T1-2NxMx. The level of expression of the cytokine (TNF), growth factors (TGFB1, CSF1, CSF2, VEGFA) and the HIF1A genes was assessed in the samples obtained using quantitative real-time PCR. Results. The study demonstrated the absence of the expression of the growth factor CSF2 gene in tumor cells of IC NST, and the expression of the CSF1 gene, independent from the metastasis status and tumor structure type. The prevalence of the expression of the VEGFA and TGFB1 genes was revealed in the alveolar and solid structures along with the rare expression of the TNF gene. Conclusions. The expression of pre-metastatic niche genes in the tumors of patients with IC NST is heterogeneous. The hypoxia-mediated change in the cytokine gene expression may be expected in the alveolar and solid structures, which ultimately results in the formation of microenvironment, facilitating tumor growth and the formation of tumor metastatic potential. K e y w o r d s: breast cancer, pre-metastatic niche, morphological heterogeneity.

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The Concept of a Preniche for Localization of Future Metastases

Cited by 3 publications

References 34 publications

Heterogeneity of Circulating Tumor Cells in Neoadjuvant Chemotherapy of Breast Cancer

Heterogeneity of Circulating Tumor Cells in Neoadjuvant Chemotherapy of Breast Cancer

Principles of premetastatic niche formation

Heterogeneity of premetastatic niches gene expression in breast cancer cells

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