The CONCENTRATIONS OF URINARY OESTRONE-3-GLUCURONIDE, LH AND PREGNANEDIOL-3α-GLUCURONIDE AS INDICES OF OVARIAN FUNCTION

Collins, W. P.; Collins, P. O.; Kilpatrick, M. J.; Manning, Patrice A.; Pike, Julie; Tyler, J. P. P.

doi:10.1530/acta.0.0900336

Cited by 101 publications

(32 citation statements)

References 2 publications

(2 reference statements)

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“…Urine collection is noninvasive, poses minimal infectious disease risk to participants and researchers, and provides sufficient volume for multiple assays and future research. Urine specimens are ideally suited for large studies because they can be collected and stored by participants, and compliance is high (1)(2)(3). Urine also provides integrated hormone measures without the confounding effects of pulsatile secretion (4 ).…”

mentioning

confidence: 99%

Comparison of Specific Gravity and Creatinine for Normalizing Urinary Reproductive Hormone Concentrations

Miller¹,

Brindle²,

Holman³

et al. 2004

Clinical Chemistry

277

211

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Background: Specific gravity (SG) may perform as well as creatinine (CR) correction for adjusting urinary hormone concentrations, as well as offer some advantages. We compared the two methods and applied them to US and Bangladeshi specimens to evaluate their use in different populations. Methods: Pearson correlations between serum concentrations and SG, CR, and uncorrected urinary concentrations were compared using paired daily urine and serum specimens from one menstrual cycle from 30 US women. Corrected urinary estrone conjugate and pregnanediol glucuronide concentrations were compared with serum estradiol and progesterone. Urine specimens across one menstrual cycle from 13 Bangladeshi women were used to evaluate the applicability of both methods to a nonindustrialized population. Linear mixed-effects models were used to compare CR and SG values in the Bangladeshi vs US specimens. Results: There was no significant difference between SG-corrected vs serum and CR-corrected vs serum correlations for either assay. Usable CR results were obtained for all US specimens, but 37% of the Bangladeshi specimens were below the CR assay limit of detection. The Bangladeshi sample had significantly lower CR and higher inter-and intrasubject CR variability than the US sample. Conclusions: SG is a potentially useful alternative to CR correction for normalizing urinary steroid hormone

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mentioning

confidence: 99%

Comparison of Specific Gravity and Creatinine for Normalizing Urinary Reproductive Hormone Concentrations

Miller¹,

Brindle²,

Holman³

et al. 2004

Clinical Chemistry

277

211

View full text Add to dashboard Cite

show abstract

“…Urine has several advantages over saliva, serum, and blood spots for frequent determinations of steroid hormones: collection is noninvasive and poses minimal infectious disease risk to participants and researchers; specimens can be self-collected and stored by participants; daily specimens over long periods of time are easily obtained; participant compliance is high; and a sufficient volume of specimen can be collected for multiple assays and future research (2)(3)(4). Furthermore, urine provides integrated hormone measures without the confounding effects of pulsatile secretion (1 ).…”

mentioning

confidence: 99%

Urinary Estrone Conjugate and Pregnanediol 3-Glucuronide Enzyme Immunoassays for Population Research

O’Connor

Brindle²,

Holman³

et al. 2003

Clinical Chemistry

102

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Background:Monitoring of reproductive steroid hormones at the population level requires frequent measurements, hormones or metabolites that remain stable under less than ideal collection and storage conditions, a long-term supply of antibodies, and assays useful for a range of populations. We developed enzyme immunoassays for urinary pregnanediol 3-glucuronide (PDG) and estrone conjugates (E1Cs) that meet these criteria. Methods: Enzyme immunoassays based on monoclonal antibodies were evaluated for specificity, detection limit, parallelism, recovery, and imprecision. Paired urine and serum specimens were analyzed throughout menstrual cycles of 30 US women. Assay application in different populations was examined with 23 US and 42 Bangladeshi specimens. Metabolite stability in urine was evaluated for 0 -8 days at room temperature and for 0 -10 freeze-thaw cycles. Results: Recoveries were 108% for the PDG assay and 105% for the E1C assay. Serially diluted specimens exhibited parallelism with calibration curves in both assays. Inter-and intraassay CVs were <11%. Urinary and serum concentrations were highly correlated: r ‫؍‬ 0.93 for E1C-estradiol; r ‫؍‬ 0.98 for PDG-progesterone.

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“…Saliva has additional advantages due to its ease of storage, as it can be stored at -20 o C for over 6 months, at 48 o C for 7 days, or at room temperature for 48 hr with no significant change in steroid levels [92]. Furthermore, urine has several advantages over saliva, serum and milk: it can be collected in large volume that enables for multiple assays [93,94,95] and provides integrated hormone measures without the confounding effects of pulsatile secretion [37]. Additionally, the availability of steroid metabolite in higher concentration in urine (by two-to fourfold or more) than that of parent sex steroids in blood give greater advantage to the use of urine [49,85] or in women [96].…”

Section: P4 Determination In Pregnant Cattlementioning

confidence: 99%

Serum, milk, saliva and urine progesterone and estradiol profiles in crossbred (Zebu x Holstein Friesian) dairy cattle

Mekonnin¹,

Howie²,

Riley³

et al. 2017

Anim Husb Dairy Vet Sci

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Effective breeding management is a crucial tool to enhance the reproductive and productive performance of dairy cattle. To achieve this, regular monitoring of their reproductive status is important. The aims of this study were to monitor the reproductive status of crossbred dairy cattle based on progesterone (P4) concentration in serum, whole milk, saliva and urine, and estradiol in serum using quantitative laboratory ELISA tests, and to document hormonal profiles at different reproductive stages. Three hundred and thirty-six (336) crossbred (Holstein Friesian x Zebu) dairy cattle (232 cows and 104 heifers) from 47 dairy farms owned by smallholder or organized commercial dairy farmers were included in the study. Matched blood, milk and saliva samples were collected twice at 11-day intervals at afternoon milking, while urine was collected just once. Serum was separated within 48 hr of blood collection. All samples were stored at -80 o C until assayed. In both cows and heifers, P4 was higher during pregnancy than in other reproductive conditions. Estradiol was higher during estrus than at other reproductive stages. Estradiol levels were significantly higher (p<0.05) in estrus cows compared with heifers. The mean level of P4 in serum strongly correlated with the value in milk (r = 0.6368, P< 0.0001), however there was no correlation in P4 concentration between serum and saliva or between P4 in serum and in urine. Hence, determination of P4 in serum and milk using an ELISA test is a reliable and precise method to monitor reproductive status in crossbred dairy cattle.

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The CONCENTRATIONS OF URINARY OESTRONE-3-GLUCURONIDE, LH AND PREGNANEDIOL-3α-GLUCURONIDE AS INDICES OF OVARIAN FUNCTION

Cited by 101 publications

References 2 publications

Comparison of Specific Gravity and Creatinine for Normalizing Urinary Reproductive Hormone Concentrations

Comparison of Specific Gravity and Creatinine for Normalizing Urinary Reproductive Hormone Concentrations

Urinary Estrone Conjugate and Pregnanediol 3-Glucuronide Enzyme Immunoassays for Population Research

Serum, milk, saliva and urine progesterone and estradiol profiles in crossbred (Zebu x Holstein Friesian) dairy cattle

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