The concentration of isoflavones in red clover (Trifolium pratense L.) at flowering stage

Lemežienė, N.; Padarauskas, Audrius; Butkutė, B.; Cesevičienė, Jurgita; Taujenis, Lukas; Norkevičienė, Eglė; Mikaliūnienė, Jovita

doi:10.13080/z-a.2015.102.057

Cited by 41 publications

(26 citation statements)

References 23 publications

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“…Among the legume species discussed in the current paper only isoflavones present in red clover have been quantified fairly in detail (Lemežien_ e et al 2015;Ramos et al 2008;Saviranta et al 2008). The concentration of total isoflavones we found in red clover (19.6 mg/g) agrees with the range reported by Ramos et al (2008) and Saviranta et al (2008) (12.3-24.1 and 15.5-24.1 mg/g, respectively).…”

Section: Phytoestrogenssupporting

confidence: 89%

“…Data were collected and managed using the HyStar 3.2 software (Bruker). Analytes in the extracts were identified according to our recently published procedure (Lemežien_ e et al 2015;Taujenis et al 2016). Quantification was performed by external calibration and the results were expressed in mg per 1 g of the dry matter (mg/g).…”

Section: Quantification Of Phytoestrogensmentioning

confidence: 99%

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Perennial legumes as a source of ingredients for healthy food: proximate, mineral and phytoestrogen composition and antibacterial activity

et al. 2017

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Perennial legumes have been used as edible or medicinal plants since ancient times. The focus of the current study are perennial legumes-Trifolium pratense L., T. medium L., Medicago sativa L., M. lupulina L., Onobrychis viciifolia Scop., Astragalus glycyphyllos L. and A. cicer L.-of branching stage as a potential source of value-added ingredients for healthy food. Freeze-dried samples were analysed for proximal composition, mineral, isoflavone and coumestrol contents as well as for antimicrobial activity. Legumes were protein-rich (23.0/100 g on average). Mineral contents in 100 g of plant dry matter averaged: K 2.64 g, Ca 1.81 g, Mg 0.475 g, P 0.324 g, Zn 2.76 mg and Fe 37.8 mg. According to the total amount of phytoestrogens, the species ranked as follows: T. medium (34.4 mg/g)) T. pratense) O. viciifolia C M. sativa = A. cicer = M. lupulina C A. glycyphyllos (0.207 mg/ g). Extracts of legumes, especially that of O. viciifolia, exhibited noticeable potency to inhibit the growth of Grampositive and Gram-negative bacteria. Perennial legumes of branching stage can be used as protein, mineral and phytoestrogen rich source for food ingredients and supplements.

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Section: Phytoestrogenssupporting

confidence: 89%

Section: Quantification Of Phytoestrogensmentioning

confidence: 99%

Perennial legumes as a source of ingredients for healthy food: proximate, mineral and phytoestrogen composition and antibacterial activity

et al. 2017

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“…There is evidence to suggest that Astragalus species have more bioactive compounds related to isoflavones than the four isoflavones selected for quantification in this study. Among the perennial legume species, isoflavones have only been quantified in some detail in red clover [ 30 , 45 , 46 ], and to a much lesser extent in other Trifolium spp. [ 47 , 48 ] and Medicago spp.…”

Section: Resultsmentioning

confidence: 99%

Mineral and Phytochemical Profiles and Antioxidant Activity of Herbal Material from Two TemperateAstragalusSpecies

Butkutė

Dagilytė

Benetis

et al. 2018

BioMed Research International

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Only a few species of the large Astragalus genus, widely used for medicinal purposes, have been thoroughly studied for phytochemical composition. The aim of our research was to investigate the rarely studied species A. glycyphyllos L. and A. cicer L. for the distribution of mineral elements and phytochemicals in whole plants at two growth stages and in morphological fractions. We also investigated the capacity of the plant extracts to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and to chelate ferrous ions. Chemical composition and antioxidant properties depended on species, maturity, and plant part. Herbal material of A. glycyphyllos was richer in Fe, total phenolics, and flavonoids, whereas extracts of A. cicer showed a higher antioxidant activity. Young plants had more isoflavones, showed greater quenching of DPPH radicals, and exhibited better mineral profiles than flowering plants. Among plant parts, leaves were the most valuable plant material according to most characteristics investigated. Isoflavone concentration in flowers was lower than in leaves and stems. None of the Astragalus samples contained detectable amounts of the alkaloid swainsonine. The study demonstrates the potential of plant material from two Astragalus species as a valuable source of iron, phenolic substances including isoflavones, free-radical scavengers, and Fe2+ chelators for pharmaceutical use.

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“…Low levels of total isoflavones and most of the individual isoflavones were also determined in the breeding populations Rc-13/40, Rc-15/12, and Rc-15/7. Lemeziene et al (2015). These widely different results may be explained by the fact that a total isoflavone content and the content of individual isoflavones depend on many other factors in addition to a cultivar, such as a farm management, harvest timing and growing seasons, plant phenological stages, plant parts, growth conditions, sampling methodology, climatic conditions, preservation method and storage.…”

Section: Proportion (%) Of Individual Isoflavones In the Total Amountmentioning

confidence: 99%

“…Prosječni sadržaj izoflavona (μg g -1 suhe tvari) u listovima crvene djeteline. , 2199.02-15670.39 μg g -1 of DM for biochanin A, 14.70-516.91 μg g -1 of DM for genistein, 0-137.91 μg g -1 of DM for daidzein, and 9810-36360 μg g -1 of DM for total isoflavones Lemeziene et al (2015). determined the isoflavone values in the Lithuanian red clover cultivars and ecotypes and found that their contents in the leaves…”

mentioning

confidence: 99%

The characterization of isoflavone content in the Croratian red clover collection

Tucak

Popović

Horvat

et al. 2019

Poljoprivreda (Online)

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Red clover is a perennial forage crop rich in isoflavones, the bioactive compounds with a positive effect on human and animal health. The aim of the research was to determine a variation in the isoflavone contents in the Croatian red clover collection and to identify the most promising materials to be used in the breeding program for the development of new cultivars for specific purposes. Leaf samples of 29 red clover cultivars/populations (two cultivars, twenty breeding populations, and seven local populations) were collected in a full flowering stage, and the identification and quantification of isoflavones was performed using the HPLC analysis. The most common isoflavones in the red clover cultivars/populations were formononetin and biochanin A. A significant variation among the cultivars/populations in the total and individual isoflavone content was determined. The populations with very high and low contents of both the total and of the individual isoflavones were identified to be used for the breeding purposes in order to develop new forage cultivars, or for specific goals in the pharmaceutical industry.

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The concentration of isoflavones in red clover (Trifolium pratense L.) at flowering stage

Cited by 41 publications

References 23 publications

Perennial legumes as a source of ingredients for healthy food: proximate, mineral and phytoestrogen composition and antibacterial activity

Perennial legumes as a source of ingredients for healthy food: proximate, mineral and phytoestrogen composition and antibacterial activity

Mineral and Phytochemical Profiles and Antioxidant Activity of Herbal Material from Two TemperateAstragalusSpecies

The characterization of isoflavone content in the Croratian red clover collection

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