The computational methods in the development of a novel multianalyte calibration technique for potentiometric integrated sensors systems

Abstract

In recent years, integration and miniaturization of ion-selective electrodes (ISEs) have brought many benefits resulting in the possibility of simultaneous determination of the ions concentration in small volume samples. One of the key problems related to the preparation of potentiometric integrated sensors systems (PISSs) is a calibration procedure due to the necessity to calibrate each particular sensor separately. The main aim of the research was to develop a novel calibration method for PISSs fabricated with the use of an all-solid-state technology, which has been compared with other types of sensor calibration technique. The proposed algorithm concerns the method of calibration solutions composition determination for miniature ion-selective sensors before measuring in biological samples especially human saliva samples. This article also compares the parameters of ionselective sensors for two types of PISSs, including ISEs based on gold (Au) and glassy carbon (GC) electrodes. In addition, a series of measurements was performed using PISS with Au-ISEs in samples of human saliva, which were preceded by different types of sensor calibration and compared with the results obtained with the clinical analyzer. Moreover, the effect of the viscosity of calibration solutions on the ISE parameters and the lifetime of the sensors were investigated.

A miniaturized solid-contact potentiometric multisensor platform for determination of ionic profiles in human saliva

“…Detailed data can be found in Table 2. Long-term stability corresponded with our previous research described in Urbanowicz et al's work [63].…”

“…This paper presents the results of the research carried out using the miniature multisensor platform of own construction composed of solid-contact ion-selective electrodes. This work is a continuation of study on the use of miniature ISEs in biomedical research [63]. Our previous article focused on examining some factors influencing ISE response, including lifetime parameters, and also showed the algorithm that allows designing ionic composition of solutions for calibration of MP-ISEs.…”

Biomolecules and Electrochemical Tools in Chronic Non-Communicable Disease Surveillance: A Systematic Review

“…Recently Urbanowicz et al [ 41 ], reported on a very small multiple biosensing platform of 10 mm diameter, relying on solid contact ion-selective electrodes for the detection of electrolytes Na + , K + , Ca 2+ , Mg 2+ , and Cl − in fresh human saliva samples. This electrolyte evaluation is essential to manage the progression of a number of non-communicable diseases, such as type-2 diabetes mellitus and lipid profile [ 42 ].…”

Potentiometric Solid-Contact Ion-Selective Electrode for Determination of Thiocyanate in Human Saliva

“…The overall diameter of the Au-ISE with case was 2 mm and the GC-ISE 6 mm. Detailed description of the construction is given in our previous reports [68][69][70].…”