The Comprehensive Peptaibiotics Database

Abstract

Peptaibiotics are nonribosomally biosynthesized peptides, which - according to definition - contain the marker amino acid α-aminoisobutyric acid (Aib) and possess antibiotic properties. Being known since 1958, a constantly increasing number of peptaibiotics have been described and investigated with a particular emphasis on hypocrealean fungi. Starting from the existing online 'Peptaibol Database', first published in 1997, an exhaustive literature survey of all known peptaibiotics was carried out and resulted in a list of 1043 peptaibiotics. The gathered information was compiled and used to create the new 'The Comprehensive Peptaibiotics Database', which is presented here. The database was devised as a software tool based on Microsoft (MS) Access. It is freely available from the internet at http://peptaibiotics-database.boku.ac.at and can easily be installed and operated on any computer offering a Windows XP/7 environment. It provides useful information on characteristic properties of the peptaibiotics included such as peptide category, group name of the microheterogeneous mixture to which the peptide belongs, amino acid sequence, sequence length, producing fungus, peptide subfamily, molecular formula, and monoisotopic mass. All these characteristics can be used and combined for automated search within the database, which makes The Comprehensive Peptaibiotics Database a versatile tool for the retrieval of valuable information about peptaibiotics. Sequence data have been considered as to December 14, 2012.

Unique amalgamation of primary and secondary structural elements transform peptaibols into potent bioactive cell-penetrating peptides

“…T2, the fungus was grown in several types of broth media. Growth of the fungus in a modified potato dextrose broth (PDB) resulted in the generation of numerous new and known members of the malettinin family of polyketides [malettinins A (27), B (28), and F-H (16)(17)(18)(19)(20), respectively] ( Fig. 2A).…”

“…The remaining peptides consisted of a series of 11-mer lipopeptaibols (8-13), which were identified as dakwaabakains A-E (8-12) and LP237-F7 (13) (25). residue, β-alanine (BALA), which is otherwise rare among the peptaibols (16,18,30,31) (SI Appendix, Fig. S8A).…”

“…The gonanes (perhydrocyclopenta[a]phenanthrenes) are a classic example of chemical repurposing with members of this diverse natural-product family acting as hormones (e.g., progesterone), bile acids (e.g., cholic acid), membrane stabilizers (e.g., cholesterol), toxins (e.g., digitoxin), as well as serving other roles (14). However, identifying functional outliers from among a group of chemically related natural products is not trivial; it requires adding a bioassay search component to the gene-or chemistry-based search process to detect a metabolite's biological attributes.The peptaibols are a class of nonribosomal-peptide-synthetase (NRPS)-derived metabolites produced by several types of fungi (15,16). These compounds have attracted considerable interest due in large part to their antimicrobial properties and potential for biocontrol applications.…”

“…The peptaibols are a class of nonribosomal-peptide-synthetase (NRPS)-derived metabolites produced by several types of fungi (15,16). These compounds have attracted considerable interest due in large part to their antimicrobial properties and potential for biocontrol applications.…”

Peptaibol profiles of Iranian Trichoderma isolates

“…The sequence of y ions originating from the split of the labile Aib-Pro bond in MS full scan mode were predicted by calculations based on the sequences with identical masses found in the Comprehensive Peptaibiotics Database and b-ion series sequence homologies [39]. The newly described peptaibols were designated with individual identifiers.…”