The comprehensive in vitro evaluation of eight different calcium phosphates: Significant parameters for cell behavior

Šupová, Monika; Suchý, Tomáš; Sucharda, Zbyněk; Filová, Elena; Kinderen, Job N. L. M. der; Steinerová, Marie; Bačáková, Lucie; Martynková, Gražyna Simha

doi:10.1111/jace.16110

Cited by 11 publications

(11 citation statements)

References 63 publications

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“…Apatite IHA/IHB was precipitated on the organic matrix by a biomimetic approach using solutions of CaCl 2 and NaH 2 PO 4 salts at pH = 9. Lower pH records less hydroxylated forms and lower concentration of CO 3 2− anions in solution and the formation of so-called calcium-deficient apatites [25]. Another factor here is the organic phase itself, which is the determining element in precipitation, e.g., what chemical groups it can provide for precipitation, and how it can affect the pH balance in solution.…”

Section: Discussionmentioning

confidence: 99%

In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels

Chocholata

Kulda

Dvořáková

et al. 2021

IJMS

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Bone tissue engineering tries to simulate natural behavior of hard tissues. This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp. In situ synthesis resulted in calcium deficient form of HAp with lower crystallinity. The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp. For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001). It seemed to be advantageous improve the properties of the scaffold by in situ synthesizing of HAp directly in the organic matrix.

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Section: Discussionmentioning

confidence: 99%

In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels

Chocholata

Kulda

Dvořáková

et al. 2021

IJMS

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“…Another possibility, suggested in the literature, is that scaffolds could adsorbe the calcium (Ca 2+ ) from the media and this would affect cell adhesion by interfering with focal contacts formation. 30,31 In the latter case, regardless of the specific mechanism, toxicity was comparable to the H 2 O 2 effect which is a well-known and characterized toxic agent. Interestingly, after SBF incubation and HCA formation, this effect was completely eliminated in such a way that cells grew more than in the control treatments.…”

Section: Scaffold -Cells Interaction: Effect Of Scaffolds On Mc3t3-e1 Fibroblasts Cell Viabilitymentioning

confidence: 84%

“…In this sense, the B‐S treatment could induce an increase in the pH of the media and affect the cell viability. Another possibility, suggested in the literature, is that scaffolds could adsorbe the calcium (Ca 2+ ) from the media and this would affect cell adhesion by interfering with focal contacts formation 30,31 . In the latter case, regardless of the specific mechanism, toxicity was comparable to the H 2 O 2 effect which is a well‐known and characterized toxic agent.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of bioactive novel scaffold by solid‐state reaction sintering for bone regeneration

et al. 2020

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In this work we present a new type of scaffold obtained by solid‐state reaction, simultaneous sintering of a mixture of precursor oxides, carbonates, and organic substances, the latter used for pore generation. Having variable local composition, exhibits excellent overall physicochemical and bioactivity response. Open porosity is about 50%–60% and its permeability 10−11 m2. X‐ray diffraction exhibits the presence of a sodium‐calcium silicate and sodium‐calcium phosphate crystalline phases. Additionally, by mechanical compression tests the range of failure stress obtained for the scaffolds was 0.3–1.1 MPa. The bioactivity and dissolution rate of the scaffolds were evaluated by in vitro tests. After 1 week soaking in simulated body fluid, the formation of a continuous hydroxyapatite layer, which does not differentiate local compositions, was observed. Our results from cell culture tests clearly indicate that during hydroxyapatite layer formation, scaffolds do not liberate any cytotoxic substances. Moreover, cells seeded in the hydroxyapatite‐covered scaffolds grew better than the control.

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“…Analogically, the behavior of calcium also differed in various environments. When calcium phosphate ceramics was exposed to distilled and deionized water, calcium was released from the material, while in cell culture media, calcium was captured by the material and depleted from the media [51].…”

Section: Xps Analysis Of Batio3 Film On Ti39nb Substratesmentioning

confidence: 99%

Beta-Titanium Alloy Covered by Ferroelectric Coating–Physicochemical Properties and Human Osteoblast-Like Cell Response

et al. 2021

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Beta-titanium alloys are promising materials for bone implants due to their advantageous mechanical properties. For enhancing the interaction of bone cells with this perspective material, we developed a ferroelectric barium titanate (BaTiO3) coating on a Ti39Nb alloy by hydrothermal synthesis. This coating was analyzed by scanning electron and Raman microscopy, X-ray diffraction, piezoresponse force microscopy, X-ray photoelectron spectroscopy, nanoindentation, and roughness measurement. Leaching experiments in a saline solution revealed that Ba is released from the coating. A progressive decrease of Ba concentration in the material was also found after 1, 3, and 7 days of cultivation of human osteoblast-like Saos-2 cells. On day 1, the Saos-2 cells adhered on the BaTiO3 film in higher initial numbers than on the bare alloy, but they were less spread, and their initial proliferation rate was slower. These cells also contained a lower amount of beta1-integrins and vinculin, i.e., molecules involved in cell adhesion, and produced a lower amount of collagen I. This cell behavior was attributed to a higher surface roughness of BaTiO3 film rather than to its potential cytotoxicity, because the cell viability on this film was very high, reaching almost 99%. The amount of alkaline phosphatase, an enzyme involved in bone matrix mineralization, was similar in cells on the BaTiO3-coated and uncoated alloy, and on day 7, the cells on BaTiO3 film attained a higher final cell population density. These results indicate that after some improvements, particularly in its roughness and stability, the hydrothermal ferroelectric BaTiO3 film could be promising coating for improved osseointegration of bone implants.

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The comprehensive in vitro evaluation of eight different calcium phosphates: Significant parameters for cell behavior

Cited by 11 publications

References 63 publications

In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels

In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels

Fabrication of bioactive novel scaffold by solid‐state reaction sintering for bone regeneration

Beta-Titanium Alloy Covered by Ferroelectric Coating–Physicochemical Properties and Human Osteoblast-Like Cell Response

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