The composition and biomechanical properties of human cryopreserved aortas, pulmonary trunks, and aortic and pulmonary cusps

Kubíková, Tereza; Kochová, Petra; Brázdil, Jan; Spaténka, J; Burkert, Jan; Králíčková, Milena; Tonar, Zbyněk

doi:10.1016/j.aanat.2017.03.004

Cited by 15 publications

(13 citation statements)

References 27 publications

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“…The removal of cellular components from the connective tissue leaves the water-insoluble matrix, generally known as the decellularized or acellular matrix / scaffold / graft. Currently, acellular matrices are used for various purposes and they have been studied extensively for their potential roles in regenerating tissues or organs in vascular surgery, 1 abdominal surgery, 2 dentistry and facial surgery, 3 or orthopedics. 4 , 5 The acellular matrix generates physiological cues that mimic the native tissue microenvironment and is, therefore, more appropriate for re-functionalization and regeneration of a tissue compared with commonly used synthetic biomaterials.…”

Section: Introductionmentioning

confidence: 99%

What happens to an acellular dermal matrix after implantation in the human body? A histological and electron microscopic study

et al. 2018

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Acellular matrices are used for various purposes and they have been studied extensively for their potential roles in regenerating tissues or organs. The acellular matrix generates physiological cues that mimic the native tissue microenvironment. Acellular dermal matrix (ADM) is a soft connective tissue graft generated by a decellularization process that preserves the intact extracellular skin matrix. Upon implantation, this structure serves as a scaffold for donor-side cells to facilitate subsequent incorporation and revascularization. In breast reconstruction, ADM is used mainly for lower pole coverage and the shaping of a new breast. It helps control the positioning of the implant in the inframammary fold, and prevents the formation of contractile pseudocapsule around the breast implant. In this study, we provide a comprehensive histological description of ADM used for human breast reconstruction over the course of several months following implementation. Using immunohistochemical methods (a panel of 12 antibodies) coupled with optical and transmission electron microscopy, we confirmed that the original acellular dermal matrix became recolonized by fibroblasts and myofibroblasts, and also by various other free cells of the connective tissue (lymphocytes, macrophages and multinucleated giant cells, granulocytes, mast cells) after implantation into the patient’s body. Within the implanted ADM, there was a relatively rapid ingrowth of blood vessels. Lymphatic vessels were only detected in one case 9 months after the implantation of the ADM. These results suggest that lymphangiogenesis is a longer process than angiogenesis.

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Section: Introductionmentioning

confidence: 99%

What happens to an acellular dermal matrix after implantation in the human body? A histological and electron microscopic study

et al. 2018

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“…All specimens were tested at room temperature and held moistened by saline solution during the experiment. The mechanical measurement and evaluation protocol were described previously in our own studies (Kubíková et al 2016(Kubíková et al , 2017.…”

Section: Mechanical Loadingmentioning

confidence: 99%

“…3). The area fraction (A ) of elastin and collagen were evaluated by stereological morphometry using a stereological grid point test system based on the Cavalieri's method (Kochová et al 2012;Kubíková et al 2017;Mouton 2002;Tonar et al 2015).…”

Section: Histological Processing and Assessmentmentioning

confidence: 99%

“…The main constituents of the connective tissue are collagen fibrils, elastic fibres and proteoglycans (Hopkins 2005). Collagen is the major extracellular matrix (ECM) component of the valves forming approximately 50% of the total valve by dry weight, elastin comprises 13% of the ECM (Bashey et al 1967;Kubíková et al 2017). Elastic arteries (ascending aorta, pulmonary artery) consists of three layers as follows: the tunica intima (endothelium and subendothelial layer), the tunica media http://eproofing.springer.com/journals_v2/printpage.php?token=B64f3-u0HFTXqgN4QgwiRqBGJWCmbV6D49zb4z5CWCsFTbCjN1RGnw 6/38…”

Section: Introductionmentioning

confidence: 99%

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Mechanical and structural properties of human aortic and pulmonary allografts do not deteriorate in the first 10 years of cryopreservation and storage in nitrogen

et al. 2019

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Query Details 1. Please check and confirm the organisation division and organisation name are correctly identified in all affiliations and amend if necessary. 2. Vojacek (2018) has been changed to Vojacek et al. (2018) so that this citation matches the list. 3. As per the information provided by the publisher, Figs. 2 and 3 will be black and white in print; hence, please confirm whether we can add "colour figure online" to the caption. 4. Kindly check and confirm the caption and layout of Table 7 are correct. 5. Meyers (2008) has been changed to Meyers et al. (2008) so that this citation matches the list. 6. Reference Lievense et al. (1998) was provided in the reference list; however, this was not mentioned or cited in the manuscript. As a rule, if a citation is present in the text, then it should be present in the list. Please provide the location of where to insert the reference citation in the main body text.

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“…SMCs are supposed to passively maintain the integrity of arterial wall ( 8 ) rather than actively contribute to the tension. However, a recent study ( 9 ) showed that glycosaminoglycans of the ground substance produced by SMCs are of great mechanical importance, but unfortunately, neglected in many histological studies. Similarly, integrin molecules that connect SMCs to the laminin and type IV collagen of the external lamina and therefore to the whole arterial matrix show different expression patterns in the contractile phenotype (α1β1, α7β1, and α8β1 integrins) than in the synthetic phenotype (α2β1, α5β1, and αvβ3 integrins).…”

mentioning

confidence: 99%

Smooth muscle phenotype in aortic diseases: Are there other histopathological markers besides contractile myofibrils?

Tonar¹

2018

Anatol J Cardiol

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The composition and biomechanical properties of human cryopreserved aortas, pulmonary trunks, and aortic and pulmonary cusps

Cited by 15 publications

References 27 publications

What happens to an acellular dermal matrix after implantation in the human body? A histological and electron microscopic study

What happens to an acellular dermal matrix after implantation in the human body? A histological and electron microscopic study

Mechanical and structural properties of human aortic and pulmonary allografts do not deteriorate in the first 10 years of cryopreservation and storage in nitrogen

Smooth muscle phenotype in aortic diseases: Are there other histopathological markers besides contractile myofibrils?

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