The Complexity of the Phospholipid Binding Protein Annexin V

Heerde, Waander L. van; Groot, P. G. De; Reutelingsperger, Chris

doi:10.1055/s-0038-1653747

Cited by 197 publications

(101 citation statements)

References 53 publications

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“…Phosphatidylserine has a high and selective affinity for annexin V, a 35 kDa phospholipid-binding protein [29]; therefore, annexin V binding to a spermatozoon indicates that its cellular membrane is impaired. The binding process can be the result of translocation of PS from the inner to the outer leaflet of the plasma membrane, resulting in PS exposure on the external surface.…”

Section: Discussionmentioning

confidence: 99%

“…It is a major cell membrane component of macrophages and other phagocytic cell types. Annexin V has a high affinity to PS in the presence of physiological concentrations of Ca +2 and is unable to pass through intact sperm membranes [12]. Thus, annexin V binding by a sperm cell indicates that its membrane integrity has been compromised [13].…”

Section: Introductionmentioning

confidence: 99%

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Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

Dırıcan¹,

Özgün²,

Akarsu³

et al. 2008

J Assist Reprod Genet

127

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Purpose Magnetic activated cell sorting (MACS) eliminates apoptotic spermatozoa based on the presence of externalized phosphatidylserine residues. We evaluated the outcome of male fertility treatment when intracytoplasmic sperm injection (ICSI) into human oocytes was performed with non-apoptotic MACS-selected spermatozoa. Methods 196 couples were treated by ICSI following spermatozoa preparation by MACS (study group; 122 couples) or density gradient centrifugation (DGC) (control group; 74 couples). Fertilization, cleavage, pregnancy, and implantation rates were analyzed. Results The percentage of sperm with normal morphology after MACS selection was improved. Cleavage and pregnancy rates were higher, respectively, in the study group than in control. A slightly higher implantation rate was also observed in the study group. Conclusions MACS selection of human spermatozoa increased cleavage and pregnancy rates in oligoasthenozoospermic ART cases. This novel method for selecting nonapoptotic spermatozoa for ICSI is safe and reliable, and may improve the assisted reproduction outcome.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

Dırıcan¹,

Özgün²,

Akarsu³

et al. 2008

J Assist Reprod Genet

127

View full text Add to dashboard Cite

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“…Preliminary evidence suggests that annexin V (placental anticoagulant protein-I), the most abundant member of this family, may be involved in A P S (70). Generally considered an intracellular protein, annexin V has also been detected in extracellular sites, such as blood and amniotic fluid.…”

Section: Roubeymentioning

confidence: 99%

“…Generally considered an intracellular protein, annexin V has also been detected in extracellular sites, such as blood and amniotic fluid. It is not known whether extracellular annexin V is secreted by cells or results from cell injury, or if it plays a role in hemostasis (70). Autoantibodies to annexin V have been observed by some groups of investigators (71) but not others (72).…”

Section: Roubeymentioning

confidence: 99%

Immunology of the antiphospholipid antibody syndrome

Roubey

1996

Arthritis & Rheumatism

286

170

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Like the femme fatale in The Beatles' Rocky Raccoon, antiphospholipid antibodies (aPL) and the syndrome with which they are associated (thrombosis, recurrent fetal deaths, thrombocytopenia) have attracted considerable attention despite a serious nomenclature problem. Along with increasing experience in the optimal management of patients with the aPL syndrome and growing evidence that autoantibodies may play a direct pathophysiologic role, research over the past several years demonstrates that a large proportion of "antiphospholipid" autoantibodies do not, in fact, recognize phospholipids. These new data indicate that the antigenic targets of antibodies detected in conventional anticardiolipin and lupus anticoagulant assays are phospholipid-binding plasma proteins, most notably, &-glycoprotein I (&GPI) and prothrombin or complexes of these proteins with phospholipids. Further, autoantibodies to other phospholipid-binding plasma proteins and certain molecules expressed on vascular endothelium may also be associated with the antiphospholipid antibody syndrome (APS) although they are not detectable in standard aPL assays.This review summarizes recent information on the specificities of autoantibodies associated with APS and discusses the insights these data offer into the potential role of autoantibodies in the syndrome's pathophysiology. Antigenic specificities of aPL: historical perspective Current concepts of aPL date to the early part of this century and the development of nontreponemal serologic tests for syphilis (STS). In the early 1940s, Mary Pangborn identified the antigenic component of the tissue extracts used in these tests as a novel anionic phospholipid, which she named cardiolipin (1). As a result of widespread population screening for syphilis, in the 1950s it was observed that individuals with chronically false-positive STS results, when followed up for many years, often developed systemic lupus erythematosus (SLE) (2). False-positive STS results were also noted to be present in early reports of SLE patients with acquired inhibitors of in vitro phospholipid-dependent coagulation tests, in particular, the activation of prothrombin (3). Laurel1 and Nilsson investigated this association and suggested that the anticoagulant effect and the positive reactions with phospholipid antigen were ascribable to the same causal factor (4). Additional support for the hypothesis that these acquired coagulation inhibitors were directed against phospholipid came from studies demonstrating that the anticoagulant effect was enhanced when the phospholipid in the test system was diluted (5).In contrast, a number of key features of the inhibitors, subsequently termed lupus anticoagulants (6), were not compatible with phospholipid specificity and suggested an important role of plasma proteins. First, certain lupus anticoagulants were species specific, for example, they prolonged coagulation reactions of human plasma but not bovine plasma (7). Second, certain lupus anticoagulants did not inhibit phospholipid-dependent reacti...

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“…Externalization of phosphatidylserine (PS) to the outer membrane of sperm is considered early sign of apoptosis. Annexin V is a protein with a molecular weight of 35 KDa that has high affinity to PS in the presence of physiological concentrations of Ca +2 and is unable to pass through intact sperm membranes; thus, annexin V binding by a sperm cell indicates that its membrane integrity has been compromised [13,14]. MACS using annexin V conjugated with magnetic microspheres, which are exposed to a magnetic field in an affinity column, can separate apoptotic from non-apoptotic sperm.…”

Section: Introductionmentioning

confidence: 99%

High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation

Ferreyra¹

2014

JFIV Reprod Med Genet

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Purpose: DNA fragmentation in spermatozoa has been associated with poor semen quality, low fertilization rates, impaired preimplantation development, and high miscarriage rates. The objective of this study was to evaluate the effects of the use of non-apoptotic MACS-selected spermatozoa in ICSI cycles, compared to those ICSI cycles in patients with normal sperm DNA fragmentation.Methods: A total of 134 cycles of ICSI were included in the study. The study group consisted of patients with high DNA fragmentation and their spermatozoa were selected by MACS (n=57); and the control group, of patients with normal DNA fragmentation and their spermatozoa were selected by classic morphological characteristics. The fertilization rate, cleavage rate, embryo quality, pregnancy rate (PR), implantation rate (IR) and miscarriage rate (MR) were compared between groups.Results: There was no difference in the fertilization rate (74.5 and 76.5%), cleavage rate and good embryo quality at Day 3 (98.3 and 89.1%; 88.4 and 83.7%) or blastocyst formation rate (50.8 and 41.1%) for the study and control groups, respectively. PRs, IRs and MRs were similar for the study group compared to the control group (PR: 63.2 versus 45.5%; IR: 37.4 versus 28.1%; MR: 8.3 versus 17.1). The women were distributed into three groups: <35 years, 35-39 years and ≥ 40 years. Pregnancy, implantation and miscarriage rates were similar in the three evaluated groups (P: NS). Conclusions:By selecting non-apoptotic sperm by MACS, we can achieve very acceptable pregnancy and implantation rates; being a good option for couples with high sperm DNA fragmentation and repeated assisted reproduction failures. Citation: García-Ferreyra J, Villegas L, Romero R, Zavala P, Hilario R, et al. (2014) High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation. JFIV Reprod Med Genet 3: 133. doi:10.4172/2375-4508.1000133 Several studies reported an improvement in fertilization rates [15,16] and embryo quality [17][18][19] because the best sperms were selected using MACS compared to standard selection methods. On the other hand, Gil et al. [20] showed that the implantation and miscarriage rated did not vary between MACS or standard sperm selection methods; however, they did observe an improvement in pregnancy rates. High Pregnancy and Implantation Rates CanIn this study, we evaluated the effects of using MACS as a sperm selection technique in patients with high DNA fragmentation compared with patients with normal DNA fragmentation and determine the efficacy of such technique in the hopes of improving ART success rates. Materials and Methods PatientsIn this study, we analyzed 134 cycles of ICSI that were done between March 2012 and April 2014 at FERTILAB Laboratory of Assisted Reproduction (Lima, Peru), all approved by the Institutional Review Board (IRB) and the corresponding Ethics Committee. Written consent was obtained from all patients and their...

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The Complexity of the Phospholipid Binding Protein Annexin V

Cited by 197 publications

References 53 publications

Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

Immunology of the antiphospholipid antibody syndrome

High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation

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