Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

Dırıcan, Enver Kerem; Özgün, O.D.; Akarsu, Süleyman; Akın, Kadir Okhan; Ercan, Özge; Uğurlu, Mukaddes; Çamsarı, Çağrı; Kanyılmaz, Oya; Kaya, Adnan; Ünsal, Ali

doi:10.1007/s10815-008-9250-1

Cited by 127 publications

(118 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Citation: García-Ferreyra J, Villegas L, Romero R, Zavala P, Hilario R, et al (2014) High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation. JFIV Reprod Med Genet 3: 133. doi:10.4172/2375-4508.1000133 Several studies reported an improvement in fertilization rates [15,16] and embryo quality [17][18][19] because the best sperms were selected using MACS compared to standard selection methods. On the other hand, Gil et al [20] showed that the implantation and miscarriage rated did not vary between MACS or standard sperm selection methods; however, they did observe an improvement in pregnancy rates.…”

Section: Discussionmentioning

confidence: 99%

High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation

Ferreyra¹

2014

JFIV Reprod Med Genet

View full text Add to dashboard Cite

Purpose: DNA fragmentation in spermatozoa has been associated with poor semen quality, low fertilization rates, impaired preimplantation development, and high miscarriage rates. The objective of this study was to evaluate the effects of the use of non-apoptotic MACS-selected spermatozoa in ICSI cycles, compared to those ICSI cycles in patients with normal sperm DNA fragmentation.Methods: A total of 134 cycles of ICSI were included in the study. The study group consisted of patients with high DNA fragmentation and their spermatozoa were selected by MACS (n=57); and the control group, of patients with normal DNA fragmentation and their spermatozoa were selected by classic morphological characteristics. The fertilization rate, cleavage rate, embryo quality, pregnancy rate (PR), implantation rate (IR) and miscarriage rate (MR) were compared between groups.Results: There was no difference in the fertilization rate (74.5 and 76.5%), cleavage rate and good embryo quality at Day 3 (98.3 and 89.1%; 88.4 and 83.7%) or blastocyst formation rate (50.8 and 41.1%) for the study and control groups, respectively. PRs, IRs and MRs were similar for the study group compared to the control group (PR: 63.2 versus 45.5%; IR: 37.4 versus 28.1%; MR: 8.3 versus 17.1). The women were distributed into three groups: <35 years, 35-39 years and ≥ 40 years. Pregnancy, implantation and miscarriage rates were similar in the three evaluated groups (P: NS). Conclusions:By selecting non-apoptotic sperm by MACS, we can achieve very acceptable pregnancy and implantation rates; being a good option for couples with high sperm DNA fragmentation and repeated assisted reproduction failures. Citation: García-Ferreyra J, Villegas L, Romero R, Zavala P, Hilario R, et al. (2014) High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation. JFIV Reprod Med Genet 3: 133. doi:10.4172/2375-4508.1000133 Several studies reported an improvement in fertilization rates [15,16] and embryo quality [17][18][19] because the best sperms were selected using MACS compared to standard selection methods. On the other hand, Gil et al. [20] showed that the implantation and miscarriage rated did not vary between MACS or standard sperm selection methods; however, they did observe an improvement in pregnancy rates. High Pregnancy and Implantation Rates CanIn this study, we evaluated the effects of using MACS as a sperm selection technique in patients with high DNA fragmentation compared with patients with normal DNA fragmentation and determine the efficacy of such technique in the hopes of improving ART success rates. Materials and Methods PatientsIn this study, we analyzed 134 cycles of ICSI that were done between March 2012 and April 2014 at FERTILAB Laboratory of Assisted Reproduction (Lima, Peru), all approved by the Institutional Review Board (IRB) and the corresponding Ethics Committee. Written consent was obtained from all patients and their...

show abstract

Section: Discussionmentioning

confidence: 99%

High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation

Ferreyra¹

2014

JFIV Reprod Med Genet

View full text Add to dashboard Cite

show abstract

“…In addition, sperm sample parameters that are improved in the subpopulation selected by MACS include: sperm motility and morphology, sperm DNA damage and hamster oocyte penetration potential of spermatozoa (Lee et al, 2010;Said et al, 2006a;Said et al, 2006b). Clinical pregnancy data collected so far indicate that the use of MACS may be of especial value for cases of male infertility associated with high incidence of apoptotic and DNA damaged sperm (Dirican et al, 2008).…”

Section: Magnetic-activated Cell Sorting (Macs)mentioning

confidence: 99%

Methods for Sperm Selection for In Vitro Fertilization

Ortega¹,

Bosch²

2012

In Vitro Fertilization - Innovative Clinical and Laboratory Aspects

View full text Add to dashboard Cite

“…As a result, a wide variety of techniques have been tried based on current knowledge in the hope of improving pregnancy outcomes. These include externalisation of phosphotidylserine (magnetic-activated cell sorting), 32 cell charge (zeta charge), 33 maturity markers (hyaluronic acid binding) [34][35][36] and detailed morphological analysis (intracytoplasmic morphologically selection sperm injection). [37][38][39][40] .…”

Section: Sperm Function Testing and Icsimentioning

confidence: 99%

Diagnostic tools in male infertility—the question of sperm dysfunction

Barratt¹,

Mansell²,

Beaton³

et al. 2010

Asian J Androl

View full text Add to dashboard Cite

Sperm dysfunction is the single most common cause of infertility, yet what is remarkable is that, there is no drug a man can take or add to his spermatozoa in vitro to improve fertility. One reason for the lack of progress in this area is that our understanding of the cellular and molecular workings of the mature spermatazoon is limited. However, over the last few years there has been considerable progress in our knowledge base and in addressing new methods to diagnose sperm dysfunction. We review the current state of the field and provide insights for further development. We conclude that: (i) there is little to be gained from more studies identifying/categorizing various populations of men using a basic semen assessment, where an effort is required in making sure the analysis is performed in an appropriate high quality way; (ii) technological development is likely to bring the reality of sperm function testing closer to implementation into the clinical pathways. In doing this, these assays must be robust, cheap (or more appropriately termed cost effective), easy to use and clinically useful; and (iii) clinical necessity, e.g., the need to identify the highest quality spermatozoon for injection is driving basic research forward. This is an exciting time to be an andrologist and, likely, a fruitful one. Keywords: gamete biomarker; male fertility; sperm biomarker; sperm dysfunction INTRODUCTION Infertility is a significant global problem affecting approximately 80 million (1:7) couples worldwide. 1 In a landmark study by Mike Hull and colleagues, in which a representative British population was studied, sperm dysfunction (lacking 'normal' function) was identified as the single most common cause of infertility. 2 Subsequent studies have confirmed these observations 3 and highlighted dysfunctional cells in men with 'normal' semen parameters and conversely normal sperm function in oligozoospermic men. 4 What is remarkable is that, for this group, there is no drug a man can take or add to his spermatozoa in vitro to improve fertility. The only option is assisted reproductive technology (ART) which usually consists of a graduation of treatment depending on severity, i.e., intrauterine insemination for mild, in vitro fertilisation (IVF) for moderate and intracytoplasmic sperm injection (ICSI) for men with severe sperm dysfunction. One reason for the lack of progress in this area is that our understanding of the cellular and molecular workings of the mature spermatazoon is limited. However, over the last few years there has been considerable progress in our knowledge base and in addressing new methods to diagnose sperm dysfunction. The purpose of this paper is to review the current state of the field and provide insights for further development. The initial focus is on the value of semen analysis as a clinical tool with the discussion progressing to examining sperm dysfunction in detail.

show abstract

Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

Cited by 127 publications

References 33 publications

High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation

High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation

Methods for Sperm Selection for In Vitro Fertilization

Diagnostic tools in male infertility—the question of sperm dysfunction

Contact Info

Product

Resources

About