2021
DOI: 10.1128/msystems.00893-21
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The Complex Transcriptional Landscape of Magnetosome Gene Clusters in Magnetospirillum gryphiswaldense

Abstract: Magnetosomes have emerged as a model system to study prokaryotic organelles and a source of biocompatible magnetic nanoparticles for various biomedical applications. However, the lack of knowledge about the transcriptional organization of magnetosome gene clusters has severely impeded the engineering, manipulation, and transfer of this highly complex biosynthetic pathway into other organisms.

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Cited by 9 publications
(10 citation statements)
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References 61 publications
(110 reference statements)
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“…This indicates the presence of an internal transcription start site (TSS) and its associated promoter within the coding sequence of mamO instead of the full transcription of the gene. Localization of an active promoter within mamO was recently described in MSR-1, suggesting that the transcriptional organization of MGCs may be more broadly conserved across MTB than assumed before 39 .…”
Section: Resultsmentioning
confidence: 88%
See 1 more Smart Citation
“…This indicates the presence of an internal transcription start site (TSS) and its associated promoter within the coding sequence of mamO instead of the full transcription of the gene. Localization of an active promoter within mamO was recently described in MSR-1, suggesting that the transcriptional organization of MGCs may be more broadly conserved across MTB than assumed before 39 .…”
Section: Resultsmentioning
confidence: 88%
“…Localization of an active promoter within mamO was recently described in MSR-1, suggesting that the transcriptional organization of MGCs may be more broadly conserved across MTB than assumed before 39 .…”
Section: Rnaseq Reveals Poor Expression Levels and Antisense Transcri...mentioning
confidence: 88%
“…In R. sphaeroides , the mic60□orf52 gene pair is co-transcribed as indicated by a transcription start site (TSS) in the intergenic region between hemD and mic60 (Myers et al 2020) (Fig 2A). In the magnetosome gene island (MAI) of Magnetospirillum gryphiswaldense, mic60 and orf52 are also co-transcribed separately from their neighboring genes (Dziuba et al 2021). The conserved motif order and composition, as well as transcriptional coupling, of Mic60 and Orf52 indicate that these proteins have structural roles and may physically interact with each other at alphaproteobacterial envelopes.…”
Section: Resultsmentioning
confidence: 99%
“…Transfer of all five mam - and mms -operons (MagOPs) from M. gryphiswaldense , conferred magnetosome biosynthesis to various foreign, hitherto non-magnetic bacteria, thereby confirming the essential role of this gene set [ 16 , 17 ]. A recent analysis by RNA-sequencing, bioluminescence reporter assays and promoter knockouts revealed that the transcriptional architecture of magnetosome operons is complex [ 18 ]: in microaerobically grown cells, the mamGFDCop (2.1 kb) and feoAB1op (2.4 kb) operons are transcribed as single transcriptional units, whereas multiple transcription start sites (TSS) were present in the mms6op (3.6 kb), mamXYop (5 kb) and the long mamABop (> 16 kb), which comprises 17 genes and encodes all the essential factors for magnetosome biosynthesis [ 13 , 19 ].…”
Section: Introductionmentioning
confidence: 99%
“…However, although these previous studies already revealed valuable insights into the transcriptional organization and the role of oxygen, major parts of the regulation and transcriptional architecture are still unknown. Most importantly, previous studies [ 18 , 28 ] employed microoxic conditions supporting fastest growth, but suboptimal magnetosome formation, as indicated by the formation of fewer, less regular and smaller magnetosomes compared to anoxic conditions [ 25 , 26 ]. Furthermore, the operon architecture and transcriptional organization of genes involved in magnetosome biosynthesis outside the MAI has remained unknown.…”
Section: Introductionmentioning
confidence: 99%