The complete sequence of a 10·8kb fragment to the right of the chromosome III centromere of Saccharomyces cerevisiae

Abstract

The complete nucleotide sequence of the D10H fragment (10850 bp) was determined. The D10H fragment is located on the right arm of chromosome III near the centromere and contains the SUF2 gene. Six open reading frames (ORFs) larger than 300 bp were found. One of them is the CIT2 gene encoding the cytoplasmic citrate synthase. The others are new putative genes and show no significant similarity with any known gene. In addition two tRNA genes (Asn and Pro) and a solo delta element were identified. Two ORFs were disrupted; no peculiar phenotype was observed.

Molecular Cloning of Chromosome I DNA from Saccharomyces cerevisiae: Characterization of the 54 kb Right Terminal CDC15-FLO1-PHO11 Region

“…These ORFs represent six of the 16 known members of the YCR7 gene family (Biteau et al, 1992;Bussey et al, 1995). Other members are on chromosomes II, III, VII, VIII and XI.…”

“…Deletion analysis indicated that the chromosome I genes are not essential for growth, mating or sporulation. YCR7 is also not essential and deletions have no obvious phenotype (Biteau et al, 1992). Introduction of a YCR7 deletion into a 3LEU2 strain deleted for the chromosome I cluster also produced no obvious phenotype (A.…”

Surface signaling in pathogenesis.

“…Cutinase transcription in this nuclear preparation required a preincubation of the preparation with the cutin monomer and the protein factor for -30 min (52). That (54,55), as well as to human (56) and frog (57) cdk2 and human cdk3 (58); Fusarium kinase 26 to Schizosaccharomyces pombe RAN1, negative regulator of sexual conjugation and meiosis (59), as well as to certain cell-division-control proteins-CC21 from peas (60), CC22 from rice (61), and CC2 from alfalfa (62). The third Fusarium kinase, PTK23, showed a high degree of homology to Candida albicans extracellular signal-regulated kinase 1 (ERK1) (63), human ERK2 (64,65), and mitogenassociated kinases from Saccharomyces cerevisiae (KSS1) (66,67), Sch.…”

Molecular Characterization of Alternaria Alternata and Cladosporium Herbarum Allergens

“…YCP4 in Saccharomyces cerevisiae was identified by genome sequencing and has no known function. It is a non-essential yeast gene (8). A possible function for the YCP4 gene is indicated by the recent finding that the Schizosaccharomyces pombe homolog of YCP4.…”