The complete nucleotide sequence of a Spanish isolate of Citrus psorosis virus: comparative analysis with other ophioviruses

Abstract

The complete genomic sequence (11278 nt) of Citrus psorosis virus (CPsV), isolate P-121 from Spain, was determined and compared with those from isolate CPV-4 and from other ophioviruses. The three RNAs of P-121 had similar size and identical organization as those of CPV-4. The 24K and the RdRp proteins were potentially encoded in the viral complementary (vc) strand of RNA 1, the 54K protein potentially encoded in vcRNA 2 and the coat protein encoded in vcRNA 3. These four proteins from P-121 and CPV-4 had 87, 92, 93 and 94% amino acid identity, respectively, but only 22, 38, 25 and 33% identity with their homologous proteins from Mirafiori lettuce big vein virus (MLBVV), the only other ophiovirus completely sequenced. Biological and genetic differences between CPsV and MLBVV (and the other ophioviruses), would support their future allocation in different genera within a tentative family Ophioviridae.

Recent advances in Citrus psorosis virus

“…Near the end of the intergenic region, a typical AAUAAA polyadenylation signal is found 12 nt upstream of the CA start polyadenylation site [39]. However, these sequences were not found for the CPsV Spanich isolate P-121 [34], neither for MiLBVV (intergenic region of 147 nt, 66 % of A ? U), and LRNV (intergenic region of 80 nt, 65 % of A ?…”

DETECTION OF Citrus psorosis ophiovirus IN CITRUS CV. NAVEL ORANGE IN EGYPT

“…Virus particles are formed by three single-stranded, negative sense RNAs and a coat protein (CP) of 48 kDa (Derrick et al, 1988;Martı´n et al, 2005). RNA1 contains 8184 bp and its complementary strand has two ORFs potentially encoding a 24 kDa protein of unknown function and a 280 kDa protein with motifs characteristic of RNA-dependent RNA polymerases (RdRp).…”

“…RNA1 contains 8184 bp and its complementary strand has two ORFs potentially encoding a 24 kDa protein of unknown function and a 280 kDa protein with motifs characteristic of RNA-dependent RNA polymerases (RdRp). RNA2 contains 1644 bp and its complementary strand encodes a 54 kDa protein without similarities to other known proteins and RNA3 contains 1454 bp and its complementary strand encodes the CP (Barthe et al, 1998;Sa´nchez de la Torre et al, 1998& 2002Naum-Onganı´a et al, 2003;Martı´n et al, 2005).…”

Development of real-time PCR based assays for simultaneous and improved detection of citrus viruses

“…CPsV, the type species of the genus Ophiovirus, has a genome consisting of three single-stranded RNAs of negative polarity that have been totally sequenced (Martín et al 2005), while CVV, a definitive species of the genus Ilarvirus, family Bromoviridae has a tripartite, positive-sense single-stranded RNA genome (Scott and Ge 1995;Li et al 2008).…”