The Complete Nucleotide Sequence of Bacteriophage HP1 DNA

Esposito, Dominic; Fitzmaurice, Wayne P.; Benjamin, Robert C.; Goodman, Steven D.; Waldman, Alan S.; Scocca, John J.

doi:10.1093/nar/24.12.2360

Cited by 64 publications

(52 citation statements)

References 47 publications

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“…The genomes of phages HP1 and HP2 in Haemophilus influenzae (30), ⌽CTX in Pseudomonas aeruginosa (31), and K139 in Vibrio cholerae (32) have all been sequenced and shown to be P2-like with respect to genome organization as well as nucleotide sequence. Phages PSP3 in Salmonella enterica serovar Potsdam (33) and SopE⌽ in Salmonella enterica serovar Typhimurium (34) are also P2-like.…”

Section: Discussionmentioning

confidence: 99%

Evolution of a Self-Inducible Cytolethal Distending Toxin Type V-Encoding Bacteriophage from Escherichia coli O157:H7 to Shigella sonnei

Allué-Guardia

Imamovic

Muniesa

2013

J Virol

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Some cdt genes are located within the genome of inducible or cryptic bacteriophages, but there is little information about the mechanisms of cdt transfer because of the reduced number of inducible Cdt phages described. In this study, a new self-inducible Myoviridae Cdt phage (⌽AA91) was isolated from a nonclinical O157:H7 Shiga toxin-producing Escherichia coli strain and was used to lysogenize a cdt-negative strain of Shigella sonnei. We found that the phage induced from S. sonnei (⌽AA91-ss) was not identical to the original phage. ⌽AA91-ss was used to infect a collection of 57 bacterial strains, was infectious in 59.6% of the strains, and was able to lysogenize 22.8% of them. The complete sequence of ⌽AA91-ss showed a 33,628-bp genome with characteristics of a P2-like phage with the cdt operon located near the cosR site. We found an IS21 element composed of two open reading frames inserted within the cox gene of the phage, causing gene truncation. Truncation of cox does not affect lytic induction but could contribute to phage recombination and generation of lysogens. The IS21 element was not present in the ⌽AA91 phage from E. coli, but it was incorporated into the phage genome after its transduction in Shigella. This study shows empirically the evolution of temperate bacteriophages carrying virulence genes after infecting a new host and the generation of a phage population with better lysogenic abilities that would ultimately lead to the emergence of new pathogenic strains.

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Section: Discussionmentioning

confidence: 99%

Evolution of a Self-Inducible Cytolethal Distending Toxin Type V-Encoding Bacteriophage from Escherichia coli O157:H7 to Shigella sonnei

Allué-Guardia

Imamovic

Muniesa

2013

J Virol

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“…H0105 is similar to a hypothetical adenine-specific DNA methylase of bacteriophage HP1 (38% identity) (Esposito et al, 1996). It has been proposed that such a DNA modification is related to bacterial virulence (Heithoff et al, 1999).…”

Section: Identification Of the Attachment Site Of Vt2-sakaimentioning

confidence: 96%

Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived from the Sakai outbreak.

et al. 1999

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The enterohemorrhagic Escherichia coli (EHEC) O157:H7 strain RIMD 0509952, derived from an outbreak in Sakai city, Japan, in 1996, produces two kinds of verotoxins, VT1 and VT2, encoded by the stx1 and stx2 genes. In the EHEC strains, as well as in other VT-producing E. coli strains, the toxins are encoded by lysogenic bacteriophages. The EHEC O157:H7 strain RIMD 0509952 did not produce plaque-forming phage particles upon inducing treatments. We have determined the complete nucleotide sequence of a prophage, VT2-Sakai, carrying the stx2A and stx2B genes on the chromosome, and presumed the putative functions of the encoded proteins and the cis-acting DNA elements based on sequence homology data. To our surprise, the sequences in the regions of VT2-Sakai corresponding to the early gene regulators and replication proteins, and the DNA sequences recognized by the regulators share very limited homology to those of the VT2-encoding 933W phage carried by the EHEC O157:H7 strain EDL933 reported by Plunkett et al. (J. Bacteriol., p1767-1778, 181, 1999, although the sequences corresponding to the structural components are almost identical. These data suggest that these two phages were derived from a common ancestral phage and that either or both of them underwent multiple genetic rearrangements. An IS629 insertion was found downstream of the stx2B gene and upstream of the lysis gene S, and this might be responsible for the absence of plaque-forming activity in the lysate obtained after inducing treatments.

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“…Further sequence database searches with BLASTP+BEAUTY revealed that the protein encoded by ORF178 had significant homology with the lysozymes of Haemophilus influenzae phage HP1 (53 % similarity, 36 % identity; Benjamin et al, 1984;Esposito et al, 1996), E. coli phage P1 (51 % similarity, 33 % identity; Schmidt et al, 1996), APSE-1, a bacteriophage from an endosymbiont of the aphid Acyrthosiphon pisum (49 % similarity, 33 % identity; van der Wilk et al, 1999), and Salmonella typhimurium phage P22 (55 % similarity, 37 % identity; . The lysozymes from HP1, P1 (protein gp17), P22 (gp19) and APSE-1 (endolysin P13) share structurally conserved regions marked in the alignment with ORF178 (Fig.…”

Section: Sequence Analysis Of Orf178 and Orf119mentioning

confidence: 99%

Expression of bacteriophage ϕEa1h lysozyme in Escherichia coli and its activity in growth inhibition of Erwinia amylovora

2004

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A 3·3 kb fragment from Erwinia amylovora phage ϕEa1h in plasmid pJH94 was previously characterized and found to contain an exopolysaccharide depolymerase (dpo) gene and two additional ORFs encoding 178 and 119 amino acids. ORF178 (lyz) and ORF119 (hol) were found to overlap by 19 bp and they resembled genes encoding lysozymes and holins. In nucleotide sequence alignments, lyz had structurally conserved regions with residues important for lysozyme function. The lyz gene was cloned into an expression vector and expressed in Escherichia coli. Active lysozyme was detected only when E. coli cells with the lyz gene and a kanamycin-resistance cassette were grown in the presence of kanamycin. Growth of Erw. amylovora was inhibited after addition of enzyme exceeding a threshold for lysozyme to target cells. When immature pears were soaked in lysates of induced cells, symptoms such as ooze formation and necrosis were retarded or inhibited after inoculation with Erw. amylovora.

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The Complete Nucleotide Sequence of Bacteriophage HP1 DNA

Cited by 64 publications

References 47 publications

Evolution of a Self-Inducible Cytolethal Distending Toxin Type V-Encoding Bacteriophage from Escherichia coli O157:H7 to Shigella sonnei

Evolution of a Self-Inducible Cytolethal Distending Toxin Type V-Encoding Bacteriophage from Escherichia coli O157:H7 to Shigella sonnei

Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived from the Sakai outbreak.

Expression of bacteriophage ϕEa1h lysozyme in Escherichia coli and its activity in growth inhibition of Erwinia amylovora

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