The complete mitochondrial genome of Microhyla pulchra (Amphidia, Anura, Microhylidae)

Abstract

The complete mitochondrial genome of Microhyl pulchra was determined in this work. This mitogenome was 16,744 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region (CR). The following four distinctive features were observed: a protein-coding gene (ND1) began with GTG as start codon; eight protein-coding genes (ND1, COII, ATP6, COIII, ND3, ND4, ND5 and Cytb) ended with incomplete stop codon T; four tRNA genes positions (tRNA-Leu (CUN)/tRNA-Thr/Trna-Pro/tRNA-Phe) located between CR and 12S rRNA genes, which was a novel mtDNA gene rearrangement in amphibians; there was no significant repeat regions in the CR.

The complete mitochondrial genome of the Kaloula verrucosa (Anura: Microhylidae) and phylogenetic analyses

“…MG962359), which included 13 PCGs, two ribosomal RNA genes, 22 transfer RNA (tRNA) genes, and a control region (D-loop). The gene organization was similar to that observed in most microhylids (Wu et al 2014;Deng et al 2016;Wang et al 2016). The overall base composition was 29.65% A, 30.77% T, 25.41% C, and 14.17% G. The AT content (60.42%) was much higher than that of GC (39.58%).…”

“…Kaloula Gray 1831 is a genus of 17 species, showing a broad distributional range in Korea and northern China to Lesser Sundas and Philippines, Bangladesh, and India, controversially in Nepal (Frost 2018). However, only three species (Kaloula pulchra AY458595, Kaloula borealis NC_020044, K. rugifera KP682314) of this genus were determined the complete mtDNA sequence (Hwang and Lee 2012;Wu et al 2014;Deng et al 2016). Therefore, more complete mitochondrial genome species would improve our understanding for further genetic research and conservation of this genus.…”

Genomic data reveals potential for hybridization, introgression, and incomplete lineage sorting to confound phylogenetic relationships in an adaptive radiation of narrow‐mouth frogs

“…; Wu et al. ), with calibration estimates from van der Meijden et al. () (species and calibration points: Supplementary Material 1).…”

“…We linked the tree model across partitions, with a separate clock rate for each of the partitions. To obtain age estimates for the MRCA of the Philippine Kaloula clade, we included previously published Microhylidae mitogenomes (Zhang et al 2005(Zhang et al , 2013Igawa et al 2008;Wu et al 2016), with calibration estimates from van der Meijden et al (2007) (species and calibration points: Supplementary Material 1). We used mitogenomic data to obtain age estimates for the MRCA of the Philippine Kaloula clade, because UCE data were unavailable for the calibration points of van der Meijden et al (2007).…”

The complete mitogenome of Microhyla fissipes (Anura: Microhylidae) and phylogenetic analysis using GenBank data mining

“…The ornamented pygmy frog Microhyla fissipes (Anura: Microhylidae) is broadly distributed in southern and central China (including Taiwan and Hainan Island) northeast of the Red River Valley (from southern Yunnan northward and east to Shanxi and Shaanxi) (Frost 2019). To date, several species (M. butleri, M. heymonsi, M. mixture, M. okinavensis, M. pulchra, and M. taraiensis) of this genus have been determined the complete mitogenome sequence and the related phylogenetic relationships have also been investigated (Zhang et al 2005;Wang et al 2016;Wu et al 2016;Yong et al 2016;Khatiwada et al 2018;Zhao et al 2018). Herein, we use mitogenome sequence under Maximum-Likelihood (ML) and Bayesian inference (BI) criteria to elucidate the relationship of M. fissipes to other Microhyla.…”