The Complete Mitochondrial Genome of Four Hylicinae (Hemiptera: Cicadellidae): Structural Features and Phylogenetic Implications

Abstract

To reveal mtgenome characterizations and reconstruct phylogenetic relationships of Hylicinae, the complete mtgenomes of four hylicine species, including Nacolus tuberculatus, Hylica paradoxa, Balala fujiana, and Kalasha nativa, were sequenced and comparatively analyzed for the first time. We also carried out the richest (11) subfamily sampling of Cicadellidae to date, and reconstructed phylogenetic relationships of Membracoidea among 61 species based on three datasets using maximum likelihood and Bayesian inference analyses. All new sequenced mtgenomes are molecules ranging from 14,918 to 16,221 bp in length and are double stranded, circular in shape. The gene composition and arrangement of these mtgenomes are consistent with members of Membracoidea. Among 13 protein-coding genes, most show typical ATN start codons and TAR (TAA/TAG) or an incomplete stop codon T–, and several genes start by TTG/GTG. Results of the analysis for sliding window, nucleotide diversity, and nonsynonymous substitution/synonymous substitution indicate cox1 is a comparatively slower-evolving gene while atp8 is the fastest gene. In line with previous researches, phylogenetic results indicate that treehopper families are paraphyletic with respect to family Cicadellidae and also support the monophyly of all involved subfamilies including Hylicinae. Relationships among the four hylicine genera were recovered as (Hylica + (Nacolus + (Balala + Kalasha))).

Mitogenomics of five Olidiana leafhoppers (Hemiptera: Cicadellidae: Coelidiinae) and their phylogenetic implications

“…This result confirms that Ledrinae is an ancient group of leafhoppers. This result is in agreement with that reported by Chen et al (2019), Wang et al (2019a) and Wang et al (2019b) and differs from that reported by Du, Dai & Dietrich (2017a), Du et al (2017b) and Tang, Huang & Zhang (2020). Moreover, Tang, Huang & Zhang (2020) -S7).…”

“…This result is in agreement with that reported by Chen et al (2019), Wang et al (2019a) and Wang et al (2019b) and differs from that reported by Du, Dai & Dietrich (2017a), Du et al (2017b) and Tang, Huang & Zhang (2020). Moreover, Tang, Huang & Zhang (2020) -S7). The phylogenetic results are different from those of similar recent studies, for which there are two possible reasons: (1) we included all 13 PCGs and 2 rRNA genes in the phylogenetic analysis, whereas previous studies included 13 PCGs or selected a small number of species;…”

Peer Review #1 of "Mitogenomics of five Olidiana leafhoppers (Hemiptera: Cicadellidae: Coelidiinae) and their phylogenetic implications (v0.3)"

“…This result is in agreement with that reported by Chen et al (2019) and Wang et al (2019a, b) and differs from that reported by Du et al (2017a, b) and (Tang et al, 2020). Moreover, Tang et al (2020) reported that there is a stable relationship between the subfamilies [(Coelidiinae + Iassinae) + Hylicinae]. The results of the present study do not show such phylogenetic relationships but rather indicate that Deltocephalinae and Hylicinae are sister groups (Figs.…”

“…This result confirms that Ledrinae is an ancient group of leafhoppers. This result is in agreement with that reported by Chen et al (2019) and Wang et al (2019a, b) and differs from that reported by Du et al (2017a, b) and (Tang et al, 2020). Moreover, Tang et al (2020) reported that there is a stable relationship between the subfamilies [(Coelidiinae + Iassinae) + Hylicinae].…”

Characterization of Two Complete Mitochondrial Genomes of Atkinsoniella (Hemiptera: Cicadellidae: Cicadellinae) and the Phylogenetic Implications

“…Moreover, there were some kinds of unmatched base pairs (Figures 4 and 5). These two structural characteristics are also present in other leafhoppers [40,48,49,[66][67][68][69]. In the predicted secondary structure, the length of the anticodon loop of all tRNAs was highly conserved for 7 bp, compared to the variable sizes of the DHU and TΨC loops.…”