The complete mitochondrial genome of the novel hybrid grouper (Epinephelus lanceolatus $ and E. moara #) includes 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNA genes, and 1 control region (D-loop), for a total length of 16,743 bp. The overall nucleotide compositions encoded on the heavy strand are 29.73% A, 28.84% C, 15.08% G, and 26.64% T.