The complete chloroplast genome ofSenna alata(L.) Roxb., an important medicinal plant from the Philippines

Abstract

Senna alata , a flowering shrub, is widely cultivated in the Philippines for its anti-fungal properties. Despite this, its chloroplast genome is not yet established. We assembled and annotated the complete chloroplast genome of accession from the germplasm collection of the Institute of Crop Science, University of the Philippines, Los Baños, using Illumina sequencing data. The complete cp genome was 159,176-bp long characterized by a large single copy of 88,769 bp, short single-copy of 18,301 bp and a pair of inverted repeat regions of 26,053 bp each. The overall GC content of the chloroplast genome was 36.4%. The plastome comprised 37 tRNA genes, 8 rRNA genes and 78 mRNA genes. Phylogenetic analysis showed that S. alata is closely related to S. siamea .

Liquid-nitrogen-free CTAB DNA extraction method from silica-dried specimens for next-generation sequencing and assembly

“…2 ). Protocol 1, which utilizes fresh leaves ground in liquid nitrogen, was used for extracting DNA from V. trifolia [ 10 ], Citrus micrantha [ 11 ], V. parviflora [ 12 ], Mentha ˣ villosa [ 13 ], V. bicolor [ 14 ], Senna alata [ 15 ] and Allium chinense [ 16 ]. The assembled chloroplast genomes are used as plant super-barcodes to elucidate the evolutionary relationship of closely related species [ 14 ].…”