2010
DOI: 10.1098/rsif.2010.0022
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The competitive adsorption of human proteins onto natural-based biomaterials

Abstract: This study aims to further the understanding of nanoscale structures relevant for cellular recognition on contact and interaction with natural-based materials. The correlation between surface characteristics and protein adsorption from unitary and complex protein systems was investigated with respect to altering the bulk chemistry of the substrate material. Polymeric blends of starch and cellulose acetate, polycaprolactone (SPCL) and ethylene vinyl alcohol (SEVA-C) were used. Different proteins, bovine serum a… Show more

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Cited by 33 publications
(25 citation statements)
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“…The in vitro methods used to study the adsorption of protein to biomaterials are inherently limited. Studies using a single-protein solution, or even a combination of several stock proteins, do not accurately reflect the amount of protein adsorbed in vivo or the competition between different proteins (Alves et al 2010;Pegueroles et al 2012). The exposure of the biomaterials to human plasma ex vivo presents difficulties because of the need for heparinization, which may interfere with the readings of the concentration of protein.…”
Section: Introductionmentioning
confidence: 99%
“…The in vitro methods used to study the adsorption of protein to biomaterials are inherently limited. Studies using a single-protein solution, or even a combination of several stock proteins, do not accurately reflect the amount of protein adsorbed in vivo or the competition between different proteins (Alves et al 2010;Pegueroles et al 2012). The exposure of the biomaterials to human plasma ex vivo presents difficulties because of the need for heparinization, which may interfere with the readings of the concentration of protein.…”
Section: Introductionmentioning
confidence: 99%
“…When the microspheres formulated with carbohydrates were compared, the highest IgG loading and encapsulation efficiency was observed in starch group (Group S), probably due to the electrostatic interaction between protein and starch. The potential to physically adsorb proteins on starch-based biomaterials has been shown in previous studies [41,42]. .…”
Section: Protein Loading and Encapsulation Efficiencymentioning
confidence: 92%
“…Moreover, it is well known that the simplest theoretical model that can be used to describe the adsorption of a monolayer is the Langmuir model [23][24][25][26]. This model is based on a kinetic approach, assumes an energetically uniform surface and disregards the interaction between adsorbed molecules.…”
Section: Tablementioning
confidence: 99%
“…The Langmuir model assumes a dynamic adsorption process with reciprocation between adsorbed and unadsorbed protein molecules and disregards the interaction between adsorbed molecules [23][24][25][26]. The Freundlich isotherm, empirical in origin, is suitable for heterogeneous surfaces and allows interactions between adsorbed molecules and between these and the surface being involved in the dynamic adsorption process [25][26][27][28]. The Langmuir-Freundlich isotherm, also known as Sip's equation, is a combination of the Langmuir and Freundlich adsorption behaviors, found to better describe the adsorption process of several molecular systems [19,29,30].…”
Section: Adsorption Isothermsmentioning
confidence: 99%
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