The compartments of the parenchyma of the lymph nodes in newborn bull calves of domestic cattle (Bos taurus)

Gavrilin, P. N.; Gavrilina, O. G.; Kravtsova, M. V.

doi:10.15421/021727

Cited by 14 publications

(14 citation statements)

References 34 publications

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“…Для гістологічного дослідження відбирали невеликі фрагменти з кожного відділу кишечника та фіксували в 10%-вому формаліні від 24 год і більше, після зневоднювали в спиртах і заливали у парафін. Парафінові зрізи органів товщиною 3 мкм забарвлювали гематоксиліном та еозином за загальноприйнятою методикою (Horalskyi et al, 2015;Gavrilin et al, 2017). Мікропрепарати досліджували за допомогою світлового мікроскопа Leica DM 1000, фото виготовляли з використанням програми LAS V4.12.…”

Section: матеріал і методи дослідженьunclassified

Microanatomical aspects of the intestines and gut-associated lymphoid tissue of meat rabbits

Gavrilin¹,

Nikitina²

2019

TAVM

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In the paper, we consider the morphology of intestine structures and gut-associated lymphoid tissue of meat rabbits. The investigation was carried out for the rabbits of the following ages: 1, 10, 20, 30, 60 and 90 days of postnatal development. In accordance with the age groups, the samples of small and large intestines were taken and stained with hematoxylin eosin. As the result of microscopy investigations, the morphology of intestines and lymphoid tissues was studied. We found out that the most area of a microscopic section was taken up by mucosa, whereas lymphoid cells were detected mainly in lamina propria and somewhat in epithelium. We observed that immune structures of 30-day rabbits were formed in lamina propria hardly breaking the submucous layer. For the 1-day rabbits, the lymphoid tissue was detected in sacculus rotundus and vermiform appendix. Starting from the 20-th day of postnatal development, the lymphoid tissue was also observed in lamina propria and other intestinal tracts. We found specific types of lymphoid tissue and immune structures, which were typical of each age group of the rabbits. Besides, we studied the traits of lymphatic nodule development within the selected ages as well as assessing differentiation periods of lymphoid tissue. We determined that lymphoid tissue could be detected for the newborn rabbits, viz. lymphatic nodules and aggregated lymphoid nodules (Peyer's patches) begin growing from the 20-th day of postnatal development, whereas germinal centers are observed in lymphatic nodules of 60-day rabbits. With age, the number of lymphatic nodules is increasing in lymphoid plaques whereas the amount of lymphoid tissue is decreasing between the plaques. Since birth, lymphatic nodules are mostly circular-shaped. However, irregular-shaped lymphatic nodules, which are arranged in two rows, are appearing in the lymphoid plaques starting from 30-day age. On the 90th day of postnatal development, almost all lymphatic nodules within the lymphoid paques have irregular shape and may be arranged in three rows. A well-developed bloodstream vessel cab be observed in the proximity of the lymphoid tissue and lymphoid structures of each rabbit age group.

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Section: матеріал і методи дослідженьunclassified

Microanatomical aspects of the intestines and gut-associated lymphoid tissue of meat rabbits

Gavrilin¹,

Nikitina²

2019

TAVM

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“…Часточкова будова паренхіми лімфатичних вузлів із класичним типом будови внутрішньоорганного лімфатичного русла наразі досліджена у відповідних органах людини та лабораторних тварин (Vyrenkov et al, 1995), а також бика свійського (Gavrilin et al, 2017a;Gavrilin et al, 2018).…”

Section: вступunclassified

Lymphatic supply and architectonics of intranodal lymphatic channel of lymph nodes of domestic pig

Gavrilin¹,

Kolesnyk²

2019

TAVM

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The peculiarities of lymphatic supply of individual somatic lymph nodes and the architectonics of their intranodal lymphatic channel in the domestic pig are analyzed. The method of indirect interstitial injection of lymphatic vessels with a fine-disperse contrast mass (black mascara suspension in 5 % gelatin gel solution) was used. The dynamics of gradual distribution of contrast mass from the interstice to the afferent lymphatic vessels and lymphatic collectors of the lymph nodes, as well as in the sinuses of the parenchyma of the organs until the appearance of the mass in the efferent lymphatic vessels were investigated by the method of anatomical preparation. Each subunit of the somatic lymph node conglomerate receives lymph from one afferent lymphatic vessel. The afferent lymphatic vessels flow into the intra-trabecular lymphatic cisterns, which are the major intranodal lymph node collectors. The number of intra-trabecular lymphatic cisterns in lymph nodes of domestic pigs corresponds to the number of subunits that form the corresponding nodule conglomerates. Intra-tubular cisterns are connected with all the sinuses of the parenchyma of the lymph nodes through the numerous lymphatic channels. The intensity of lymph flow from the cavity of the lymphatic cistern to the sinuses of the parenchyma nodes is greater in the direction from the top of the trabecula (cistern) to the central (middle) area of the lymphoid tissue. Contrast mass sequentially fills afferent lymphatic vessels, intra-trabecular lymphatic cisterns, central sites of parenchyma of nodes, peripheral sites of the parenchyma of nodes, including marginal and portal sinuses, efferent lymphatic vessels. The efferent lymphatic vessels are localized at the hilus of the subunits of the nodes together with the neurovascular bundles. The afferent vessels are located separately at the opposite pole of the subunits. In general, lymph nodes of the domestic pig are characterized by the usual, rather than the «reverse» type of intranodal lymphodynamics. As peculiarities of the structure of the intranodal lymphatic channel are: localization of the intranodal lymphatic collector in the middle of the highly developed capsular trabeculae, reminiscent of the structure of additional hilus of the nodes, but does not contain neurovascular fascicles; preferably a «centrifugal» type of distribution of the lymph in the sinus parenchyma of the lymph nodes from apex of the cistern, and, accordingly, from the deep layers of the lymphoid parenchyma to the marginal and portal sinuses. The established type of lymphatic supply of the lymph nodes of domestic pig probably provides a more effective immune response in consequence of the rapid and simultaneous entry of antigens into different, both superficial and deep layers of the lymphoid tissue of the organs.

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“…Минимальные морфометрические характеристики зон клональной пролиферации лимфоцитов в лимфатических узлах плодов связаны с тем, что у плацентарных млекопитающих антигенный прессинг на органы на ранних этапах онтогенеза является крайне неравномерным, он лавинообразно нарастает непосредственно после рождения, в период постнатальной адаптации и имеет высокие стабильные показатели на дальнейших этапах постнатального развития (Gavrilin et al, 2017a. У плодов млекопитающих, особенно у видов с эпителио-и десмохориальным типом плаценты, в норме абсолютно непрони-цаемой для каких либо относительно крупных молекул, которые могут потенциально иметь антигенные свойства, антигенная «нагрузка» на периферические лимфоидные органы весьма незначительна (Sapin, 2006).…”

Section: 2 2unclassified

Prenatal morphogenesis of compartments of the parenchyma of the lymph nodes of domestic cattle (Bos taurus)

Gavrilin

Lieshchova

Gavrilina

et al. 2018

Regul. Mech. Biosyst.

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The article analyzes the regularities of the formation and development of the lymphoid lobules of the parenchyma of the somatic (Limphonodi cervicales superficiales) and visceral (L. jejunales) lymph nodes in domestic cattle in the fetal period of ontogenesis. We used routine histological techniques and author's modification of the impregnation of total median sections with silver nitrate. Visualization of various zones of lymphoid lobules was carried out taking into account the specific for different cell zones architectonics of reticular fibers. It has been established that signs of morphological heterogeneity of parenchyma of lymph nodes are first found in three month-old fetuses, which is associated with the concentration of lymphoid tissue along the marginal sinus. Separation of lymphoid lobules and their structural and functional differentiation are first detected in five month fetuses. In the lymphoid lobules of five month-old fetuses all structural and functional cell zones are observable, among which the regions of clonal proliferation of T and B lymphocytes are the least developed, and regions of the transit corridors for lymphocytes migrating medullary and the cords (zone of potential accumulation of plasmocytes and antibody formation) are the most developed. Structural and functional transformations of compartments in the prenatal period of ontogenesis are accompanied by a predominantly moderate increase of the relative volume of specialized T- and B-dependent zones of lobules, against a background of a gradual decrease of the volume of transit corridors for lymphocytes migrating and zone of potential accumulation of plasmocytes and antibody formation. Due to the small volume and relatively low rates of development of the lymphocytes clonal proliferation zones, the quantitative ratios of the cellular zones in lymphoid lobules of the lymph nodes of domestic cattle in prenatal ontogenesis remain relatively stable, while maintaining the maximum indices of the development of transit corridors for lymphocytes migration and medullary cords. Among the zones of lymphocytes clonal proliferation throughout the fetal period, T-dependent zones predominate, the relative volume of which is 5.0–7.5 times greater than the volume of B-dependent zones. Lymphoid lobules in the lymph nodes of the domestic cattle fetuses of all age groups are arranged along the marginal sinus in one row and have a polar structure due to the formation of lymph nodes at one pole of the lobules in the interfollicular zone. In the visceral lymph nodes (L. jejunum) of 8–9 month-old fetuses, individual lymph nodes can form in paracortical strands, on the border with the interfollicular zone.

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The compartments of the parenchyma of the lymph nodes in newborn bull calves of domestic cattle (Bos taurus)

Cited by 14 publications

References 34 publications

Microanatomical aspects of the intestines and gut-associated lymphoid tissue of meat rabbits

Microanatomical aspects of the intestines and gut-associated lymphoid tissue of meat rabbits

Lymphatic supply and architectonics of intranodal lymphatic channel of lymph nodes of domestic pig

Prenatal morphogenesis of compartments of the parenchyma of the lymph nodes of domestic cattle (Bos taurus)

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