The comparisons of Lycii Radicis Cortex and Corni Fructus water extract effects on streptozotocin-induced diabetes in rats

Worldwide spread in the incidence of diabetes justified the need of extensive research in an appropriate model system to develop an effective treatment strategy for diabetology. In recent years, because of animal ethical issues and limitations, the silkworm (Bombyx mori) has emerged as a perfect invertebrate model for various biomedical experiments. However, there are several issues related to induce hyperglycaemia in the mammalian system that have not been studied in the silkworms. In the present study, we investigated the consequences of induced hyperglycaemia by oral and intravascular administration of streptozotocin (STZ) and glucose in the silkworms. Interestingly, results confirmed the competence of STZ to induce hyperglycaemia when administered through intravascular route, but suppress the glucose concentration if administrated orally in the silkworms. Comparatively, the severity of induced hyperglycaemia in the silkworms remained nearly the same in response to the oral and intravascular administration of glucose. Furthermore, prolonged administration of STZ has recorded negative effect on the growth rate of the silkworms, whereas a drastic decline in the same was reported in the silkworms administered with glucose. Notably, these results confirm that the response shown by the silkworms to STZ is very similar to that of mammals, making it an excellent model to study diabetic complications and consequences associated with STZ in diabetology.

Physiological Entomology

Section: Introductionmentioning

confidence: 99%

Streptozotocin induced hyperglycaemia in the invertebrate diabetic model Bombyx mori

Ankola,

Chiome,

Srinivasan

et al. 2023

“…동의보감 신형문 9) 에서 삼정환(Samjung-hwan)은 "오랫 , 항산화 효과 12) , 면역개 14) . 또한 삼정환의 구성 약재인 창출(Atractylodes japonica)의 지질대사 개선 효과 15) , 지골 피(Cortex lycii radicis)의 혈당강하, 혈압강압 효과 16,17) , 상 심자(Morus alba Linne)의 항산화 효과 18,19) (Fig. 1, 2).…”

unclassified

Repeated Dose 90-Day Oral Toxicity Study of Modified Samjung-Hwan in Sprague-Dawley Rats

Lee

2018

JKOMOR

The study is aimed at evaluating the possible toxicity in 90-day repeated oral administration of modified Samjung-hwan (mSJH) in Sprague-Dawley (SD) rats. This study was conducted to detect the no-observed adverse effect level (NOAEL). Methods: Modified SJH extract was administered orally in male and female SD rats at dose of 0, 1,000, 2,000, 4,000 mg/kg. Each group consisted of 10 rats of each gender. The modified SJH extract was given once a day for 90 days. We monitored the changes of mortalities, clinical signs, body weight changes, food consumption, ophthalmologic findings, urine analysis, hematology, serum biochemistry, necropsy findings, organ weights, histological markers of all animals treated with modified SJH extract during the study period. Results: There were no toxicologically significant changes in mortalities, clinical signs, body weight gains, food consumption, ophthalmologic findings, urine analysis, hematology, serum biochemistry, necropsy findings, organ weights, histological markers in any of rats tested. Conclusions: The NOAEL of the modified SJH extract in male rats and no observed effect level (NOEL) in female rats are considered 4,000 mg/kg.

“…또한 삼정환의 구성 약재인 창출(Atractylodes japonica) 8,9) , 지골피(Cortex lycii radicis) [10][11][12][13] , 상심자(Morus alba Linne) …”

unclassified

Single Oral Dose Toxicity Study of Modified Samjung-Hwan in Sprague-Dawley Rats

Lee

2017

JKOMOR

Objectives: Samjung-hwan (SJH) is a Korean herbal formula that has been used for antilipidemic, antioxidant, and antidepressant effects. As a part of safety evaluation, single oral dose toxicity study of modified SJH in Sprague-Dawley (SD) rats was carried out. Methods: Modified SJH extract was administered orally in male and female SD rats at dose of 5,000 mg/kg. After single oral administration of modified SJH, we monitored the changes of mortalities, clinical signs, and body weight daily for 14 days. Gross necropsy findings were also evaluated at the end of the experiment. Results: Modified SJH extract did not induce any toxic signs in the changes of mortalities, clinical signs, and body weight. There were also observed no gross necropsy findings in rats. Conclusions: As results, the approximate lethal dose of modified SJH extract in SD rats was considered to be over 5,000 mg/kg. This suggests that modified SJH is safe herbal formula for the treatment with.