The Comparison of the Effect of Endodontic Irrigation on Cell Adherence to Root Canal Dentin

Ring, Karla C.; Murray, Peter E.; Namerow, Kenneth N.; Kuttler, Sergio; Garcı́a-Godoy, Franklin

doi:10.1016/j.joen.2008.09.001

Cited by 117 publications

(124 citation statements)

References 32 publications

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“…Hypochlorous acid (HOCl) is the active component, with biocompatibility and antimicrobial features against most endodontic pathogens [44][45] . Compared to NaOCl and CHX, AquatineEC™ was less toxic to dental pulp stem cells and allowed cell adherence to root walls 41 . However, further studies should be performed to better understand its properties and possible use in regenerative endodontics.…”

Section: Irrigant Solutionsmentioning

confidence: 99%

“…As for chlorhexidine, case reports have used concentrations of 2% 15,39 and 0.12% 40 . Despite satisfactory antimicrobial properties, these substances are not biocompatible, limiting the survival of dental pulp stem cells and adherence to dentinal walls 41 . In addition to irrigant solutions, chelating agents such as EDTA, citric acid and MTAD may also be used to remove the smear layer.…”

Section: Irrigant Solutionsmentioning

confidence: 99%

“…Although appearing to be a substance with some promise, it is not known if EDTA could damage the stem cell proliferation process during revascularization 44 . Taking into consideration the importance of stem cells for both revascularization and regeneration, studies have evaluated cytotoxicity and interference of chemical substances in the adhesion of stem cells 41 . NaOCl, CHX, Aquatine Endodontic Cleanser (AquatineEC), Morinda Citrifolia™ (MCJ), Sterile Saline, EDTA and MTAD were evaluated both individually and in combination.…”

Section: Irrigant Solutionsmentioning

confidence: 99%

“…It was shown that NaOCl and CHX presented cytotoxic effects, leading to a decrease in dental pulp stem cell adherence to the dentinal walls and, moreover, the presence of a smear layer did not have any influence on this adherence. AquatineEC™ is a new irrigant solution used to irrigate, clean and debride the root canal system 41 . Hypochlorous acid (HOCl) is the active component, with biocompatibility and antimicrobial features against most endodontic pathogens [44][45] .…”

Section: Irrigant Solutionsmentioning

confidence: 99%

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Pulp revascularization: an alternative treatment to the apexification of immature teeth

Albuquerque

Nagata

Soares

et al. 2014

RGO, Rev. Gaúch. Odontol.

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RESUMOA revascularização pulpar pode ser considerada atualmente como um tratamento alternativo à apicificação e é indicado para dentes com rizogênese incompleta e necessidade de tratamento endodôntico. A apicificação envolve a realização de trocas periódicas em longo prazo de uma pasta de hidróxido de cálcio com o objetivo de formar uma barreira calcificada. Apesar de ser a terapia mais empregada, a permanência desta medicação por longos períodos e as trocas sucessivas, podem levar à fragilização da raiz devido às propriedades higroscópicas e proteolíticas do hidróxido de cálcio, aumentando o risco de fraturas e contaminação do espaço pulpar. Desta forma, tem-se buscado novas alternativas de tratamento que possibilitem o fechamento do forame apical dos dentes imaturos sem que ocorra a fragilização dos mesmos. A revascularização pulpar tem surgido como uma nova opção de tratamento para estimular o término do desenvolvimento radicular e não apenas o fechamento apical. Há na literatura uma variedade de protocolos utilizando esta técnica, buscando sempre alcançar o sucesso no tratamento. Diante dessa variedade, é de grande importância a realização de uma revisão de literatura sobre revascularização pulpar visando reunir, descrever e discutir os diferentes protocolos, para que possam direcionar novas pesquisas e ser empregada de forma adequada pelo clínico. O objetivo desse trabalho é revisar na literatura os diferentes protocolos de revascularização pulpar.Termos de indexação: Polpa dentária. Endodontia. Regeneração. Pulp revascularization: an alternative treatment to the apexification of immature teethRevascularização pulpar: tratamento alternativo à apicificação de dentes jovens com rizogênese incompleta

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Section: Irrigant Solutionsmentioning

confidence: 99%

Section: Irrigant Solutionsmentioning

confidence: 99%

Section: Irrigant Solutionsmentioning

confidence: 99%

Section: Irrigant Solutionsmentioning

confidence: 99%

See 2 more Smart Citations

Pulp revascularization: an alternative treatment to the apexification of immature teeth

Albuquerque

Nagata

Soares

et al. 2014

RGO, Rev. Gaúch. Odontol.

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“…A clorexidina também é utilizada nas concentrações de 2 e 0,12%, apesar das propriedades antimicrobianas satisfatórias, estas substâncias não são biocompatíveis, podendo inviabilizar as células-tronco presentes no tecido pulpar impedindo as mesmas de se aderirem à superfície dentinária intraradicular (Ring et al, 2008). utilizaram NaOCl 2,5%.…”

Section: Substâncias Químicasunclassified

Influência de medicações intracanal utilizadas em procedimentos endodônticos regenerativos na sobrevivência de células da papila apical in vitro

Oliveira¹

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Oliveira AP. Influence of intracanal medications used in regenerative endodontic procedures on survival of apical papilla cells in vitro [thesis]. São Paulo: Universidade de São Paulo, Faculdade de Odontologia; 2014. Versão Corrigida.Regenerative Endodontic procedures have provided changes in treatment of patients with immature teeth and apical periodontitis enabling full root development and lower incidence of dental fracture. Intracanal dressings are used for disinfection; however, their effect on apical papilla cells is poorly elucidated. Additionally, the effect of these substances on cells previously subjected to proinflammatory condition is still unknown. The aim of this study was to evaluate the cytotoxicity of intracanal dressings used in Endodontics regenerative procedures on cultured human apical papilla cells at physiologic and activated condition. Cell culture was established from the apical papilla removed from an extracted immature third molar. The substances studied were triple antibiotic modified paste: ciprofloxacin, metronidazole and cephalosporin (1:1:1); CFC: ciprofloxacin, metronidazole and calcium hydroxide (1:1:2) and modified CFC: ciprofloxacin , metronidazole and calcium hydroxide (2:2:1). Part of the cells was stimulated previously with lipotheichoic acid (LTA) of Enterococcus faecalis por 7 days. Once plated, cells were exposed to increasing concentration of the medications for 1, 3, 5 and 7 days. Cell viability was evaluated by means of 3-bromide (4.5-dimetiliazol-2-yl) -2.5-difeniltetraze (MTT) and Nitric Oxide (NO) release was assessed by the Griess method. The statistical analysis was done through analysis of variance with 1 criteria (ANOVA) followed by Tukey test with 5% of significance level. Modified CFC was the medication that demonstrated the less cytotoxic effect on cell viabilityat the experimental periods studied while CFC promoted significant decrease on cell viability specially after 7 days of contact. The modified triple antibiotic paste resulted in important alteration of cell viability being considered the most citotoxic. Cellular activation by LTA resulted in increased levels of mitochondrial activity for all medications being more evident at the longer experimental periods. Cellular activation also contributed to higher levels of nitric oxide release. In conclusion, the cytotoxic effect of the tested medications is dependent on concentration, time of contact and cellular condition, being the triple antibiotic modified paste the most cytotoxic in high concentrations leading clinically in the decreased of the cells viability of the apical papilla, decreasing the success of regenerative procedures.

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Ozonization and Electrochemical Root Canal Disinfection

Pileggi

2014

Disinfection of Root Canal Systems

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The Comparison of the Effect of Endodontic Irrigation on Cell Adherence to Root Canal Dentin

Cited by 117 publications

References 32 publications

Pulp revascularization: an alternative treatment to the apexification of immature teeth

Pulp revascularization: an alternative treatment to the apexification of immature teeth

Influência de medicações intracanal utilizadas em procedimentos endodônticos regenerativos na sobrevivência de células da papila apical in vitro

Ozonization and Electrochemical Root Canal Disinfection

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