According to the
American Association of Endodontists, currently
22.3 million endodontic procedures are being performed annually with
the success rate of 70–95% and the average survival rate of
the root canal procedure is approximately 67% after 5 years and 56%
after 8 years. One of the major reason for the failure is relapse
of infection. Hence, it is imperative to develop an assistive or alternative
method to eradicate the bacterial infection effectively without affecting
patient compliance. The application of electrochemistry has been used
previously to disinfect catheters and implant disinfection. Hence,
the aim of this study is to utilize the principles of electrochemistry
to develop a microelectronic device to eradicate bacterial infection
for root canal treatment. The electrochemical protocol includes open
circuit potential (60 s) and potentiostatic scan at varying voltage
(−9 to +2 V) at a different time duration (1–5 min). Enterococcus faecalis in the form of planktonic and biofilm
was used in this study. After electrochemical treatment, the bacterial
viability was evaluated using alamarBlue assay, colony forming units,
confocal microscopy, and scanning electron microscopy. Cytotoxicity
evoked by electrochemical voltage in comparison to NaOCl solution
was performed using osteoblasts in 2D and 3D cell culture systems.
The results of the study show that the application of −2 to
+2 V at 1–5 min did not show any significant reduction in bacterial
growth. However, the cathodic voltage of −9 V for 5 min showed
a significant reduction (p < 0.001) in the bacterial
count (80–95%). Similar results were obtained from biofilm
study, which is more realistic to the in vivo condition. In contrast,
the method did not induce cytotoxicity to the cells in 3D culture
system (65% viability) in comparison to the highly toxic nature (0%
viability) of NaOCl, indicating better patient compliance. Hence,
the study provides supporting evidence to develop an electrochemically
driven microelectronic device that can be a potential assistive dental
instrument for endodontic procedures.