The comparison of DNA extraction techniques in human bone and tooth samples exposed to high heat

Yükseloğlu, Emel Hülya; Dastan, Kadir; Yonar, Fatma Cavus; Rayimoğlu, Gülten; Karataş, Ömer Faruk; İşlek, Dilek Salkım; Doğan, Muzaffer Berna

doi:10.5455/medscience.2019.08.9051

Cited by 2 publications

(3 citation statements)

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“…A high quantity of DNA can be extracted from the root portion of the tooth. Before DNA extraction, the extracted teeth may be sterilized with 0.5% sodium hypochlorite, washed with distilled water for five minutes, allowed to dry at room temperature, and subjected to ultraviolet (UV) light irradiation for one hour 28 .…”

Section: Dna Isolation Methodsmentioning

confidence: 99%

“…The phenol-chloroform isoamyl alcohol method combined with the QIAamp DNA mini kit methodis the most effective and successful method of high molecular weight DNA extraction 28 . Limitations of phenolchloroform isopropyl alcohol are it takes a longer time, uses toxic solvents, is expensive, and requires multiple tube transfers involving a high chance of contamination and mislabeling 30 .…”

Section: Dna Isolation Methodsmentioning

confidence: 99%

“…Use of chelating resins with ion-exchangeapproach like Chelex method is also in use 31 . Chelex 100™ (Medox Bio) and Qiagen DNA Mini Kit provide a much faster result, are inexpensive, does not contain more stages, recovers more DNA, and use non-toxic substances compared to organic techniques but obtaining pure DNA is difficult 28 . Modified Chelex technique recovers a higher quantity of DNA compared to classical Chelex method but is time-consuming.…”

Section: Dna Isolation Methodsmentioning

confidence: 99%

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Tooth as a Source of DNA in Forensic or Investigative Genetics: An Overview

Nandini

Joji²

2022

jfds

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Deoxyribonucleic acid (DNA) is the genetic code of most organisms including humans. In the last few years, DNA analysis methods are applied to forensic cases for human identification which is termed forensic or investigative genetics. It is often challenging to obtain and interpret DNA from routine samples like blood in severely decomposed or disfigured bodies recovered from incineration, immersion, trauma, mutilation, and decomposition as in incidents of fire, explosion, or murder. The tooth can survive any extreme environment with minimum risk of contamination making it a valuable alternative source of DNA in such cases. Tooth structures like the enamel (amelogenin protein), dentin, cementum, pulp, and adherent tissues like bone and periodontal fibers are sources from which DNA can be obtained. Various methods have been described for DNA extraction. There are numerous applications of DNA analysis like identification of the deceased or missing victim or the unknown culprit from a crime scene, solving paternity issues, determining the occurrence of any genetic disease, and determining the ancestry. This article briefly summarizes an overview of the tooth as a valuable DNA source and various methods and challenges related to DNA analysis.

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Section: Dna Isolation Methodsmentioning

confidence: 99%

Section: Dna Isolation Methodsmentioning

confidence: 99%