Effectiveness of various methods of DNA isolation from bones and teeth of animals exposed to high temperature

Grela, Małgorzata; Jakubczak, Andrzej; Kowalczyk, Marek; Listos, Piotr; Gryzińska, Magdalena

doi:10.1016/j.jflm.2021.102131

Cited by 9 publications

(3 citation statements)

References 45 publications

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“…On the other hand, there are many forensic cases of abuse of animals by humans, which intentionally injure, kill, or trade pets, farm animals, or wildlife without leaving a trace. Therefore, the investigation could involve the analysis of damaged biological materials that need a particular optimization [39]. Unfortunately, cases of animal sexual abuse (ASA) have also been increasingly reported and defined as the eroticization of violence, control, and exploitation [40].…”

Section: Animal Dna In Forensic Casesmentioning

confidence: 99%

The Revolution of Animal Genomics in Forensic Sciences

Cardinali

Tancredi

Lancioni

2023

IJMS

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Nowadays, the coexistence between humans and domestic animals (especially dogs and cats) has become a common scenario of daily life. Consequently, during a forensic investigation in civil or criminal cases, the biological material from a domestic animal could be considered “evidence” by law enforcement agencies. Animal genomics offers an important contribution in attacks and episodes of property destruction or in a crime scene where the non-human biological material is linked to the victim or perpetrator. However, only a few animal genetics laboratories in the world are able to carry out a valid forensic analysis, adhering to standards and guidelines that ensure the admissibility of data before a court of law. Today, forensic sciences focus on animal genetics considering all domestic species through the analysis of STRs (short tandem repeats) and autosomal and mitochondrial DNA SNPs (single nucleotide polymorphisms). However, the application of these molecular markers to wildlife seems to have gradually gained a strong relevance, aiming to tackle illegal traffic, avoid the loss of biodiversity, and protect endangered species. The development of third-generation sequencing technologies has glimmered new possibilities by bringing “the laboratory into the field”, with a reduction of both the enormous cost management of samples and the degradation of the biological material.

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Section: Animal Dna In Forensic Casesmentioning

confidence: 99%

The Revolution of Animal Genomics in Forensic Sciences

Cardinali

Tancredi

Lancioni

2023

IJMS

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“…In the case of catastrophes where corpses or human remains have remained in the water for a considerable time, the probability of using muscle samples or organs is frankly reduced, due to the different processes of saponification and putrefaction; in these cases, the sample of choice will be dental or bone pieces. Due to the characteristics of both bones and teeth, they are also the biological samples of choice in cases of high temperatures, such as fires and explosions (Schwark et al 2011;Krishan et al 2015;Uzair et al 2017;Grela et al 2021;Kumar 2022).…”

Section: Collection Of Evidence At the Site Of The Disaster For Genet...mentioning

confidence: 99%

Relevance of Genetic Identification and Kinship Analysis in Human and Natural Catastrophes—A Review

Real-Picado,

Díaz,

Gomes

2023

Genealogy

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Different types of disasters, whether natural or human in character, lead to the significant loss of human lives. In the latter case, the quick action of identification of corpses and human remains is mandatory. There are a variety of protocols to identify victims; however, genetics is one of the tools that allows an exact identification of the victim. However, several factors may interfere with this identification, from the biological samples’ degradation not allowing the analysis of nuclear information, to failure to dispose of biological samples from family members. Access to certain family members could be a determinant of the proper choice of genetic markers that allow the identification of the victim, or his/her inclusion in a given genetic maternal or paternal lineage. New advances in the field of genetics are soon expected to allow for the identification of victims from disasters with only their biological postmortem samples; it may also be possible to draw a robot portrait of a victim’s most likely physical characteristics. In all cases, genetics is the only modern tool with universal character and can be used in essentially all biological samples, giving and identification of more or less accurate statistical character, depending on whether nuclear or lineage markers are used.

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“…The challenge is a driving force to search for optimal biological materials for post-mortem genetic analyses [12]. Apart from searching for "the best tissue," they also attempt to find the optimal location for collecting material during an autopsy [12] and optimal isolation methods for burnt biological material [13]. In the case of burnt corpses, hard tissue is often the only material available for examination.…”

Section: Introductionmentioning

confidence: 99%

The intervertebral discs’ fibrocartilage as a DNA source for genetic identification in severely charred cadavers

Tomsia

Droździok

Banaszek

et al. 2022

Forensic Sci Med Pathol

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Identifying charred human remains poses a challenge to forensic laboratories. High temperature completely incinerates the superficial tissues and partially destroys bones, forcing the forensics to seek an alternative, for bones and teeth, forensic material that should quickly and cheaply deliver DNA of sufficient quantity and quality. We sought, other than rib cartilage, types of cartilages that could serve as a DNA source. DNA was isolated from the fibrous cartilage of a fibrous ring of intervertebral L1-L2 discs sampled from charred cadavers or charred body fragments: 5 victims of car fires, 1 victim of combustion during a residential house gas explosion, and 3 victims of nitroglycerin explosion. DNA was isolated by the column method. DNA quality and concentration were assessed by RT-PCR and multiplex PCR for 23 autosomal and 17 Y chromosome STR loci. STR polymorphism results obtained by capillary electrophoresis served for likelihood ratio (LR) calculations. DNA concentration in relation to the cadaver’s age and post-mortem interval (PMI) were analyzed. All samples (n = 9) yielded good-quality DNA in quantities (0.57–17.51 ng/µL for T. Large autosomal sequence) suitable for STR-based amplification. The isolated DNA characterized a low degradation index (0.80–1.99), and we were able to obtain complete genetic profiles. In each of the nine cases, the genotyping results allowed identifying the victims based on comparative material from the immediate family. The results demonstrate the usefulness of human intervertebral disc fibrocartilage as an alternative DNA source for the genetic identification of charred bodies or charred torso fragments.

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Effectiveness of various methods of DNA isolation from bones and teeth of animals exposed to high temperature

Cited by 9 publications

References 45 publications

The Revolution of Animal Genomics in Forensic Sciences

The Revolution of Animal Genomics in Forensic Sciences

Relevance of Genetic Identification and Kinship Analysis in Human and Natural Catastrophes—A Review

The intervertebral discs’ fibrocartilage as a DNA source for genetic identification in severely charred cadavers

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