The Comparison of Chromosome Analysis Result by Manual and Software Cytovision Image Analysis Using Simple G-Banding

Ciptadi, Gatot; Ihsan, Muhammad Nur; Rahayu, Sri; Nurgiartiningsih, Veronica Margareta Ani; Mudawamah, Mudawamah; Putri, Ardyah Ramadhina Irsanti

doi:10.21776/ub.rjls.2017.004.02.3

Cited by 5 publications

(7 citation statements)

References 8 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Por otro lado, el análisis del cariotipo de los bovinos estudiados reveló que los cromosomas de una célula y cada individuo independiente varían en tamaño y forma tanto en bovinos macho (figura 1a) y como en bovinos hembra (figura 1b). Sin embargo, tal variación pudo ser causada por factores físicos durante la preparación de las células en metafase, la fijación o la separación del cromosoma en el portaobjeto [7]. Los resultados encontrados en la presente investigación, que indican la normalidad cromosómica en la totalidad de los animales analizados, concuerdan con el estudio realizado por Ciptadi et al [7], quienes analizaron, a nivel citogenético, bovinos correspondientes a la primera generación de los cruces del ganado Madura Cattle frente a Bos taurus, en Indonesia, y encontraron el número diploide normal (2n=60), tanto estructural como en número cromosómico en todos los bovinos observados.…”

Section: Resultados Y Discusiónunclassified

“…Además, muchas de estas anomalías consisten en monosomías autosómicas, triploidía, translocaciones (recíprocas y robertsonianas en uno de los progenitores), inversiones y mosaisismo [5], [6]. En bovinos, las anomalías cromosómicas reportadas con mayor frecuencia son, primero, la fusión céntrica o translocación robertsoniana 1/29 [7] y, segundo, el quimerismo leucocitario XX/XY, condición comúnmente conocida como freemartinismo. La primera de estas ha sido identificada en más de 60 razas, en las que se reporta disminución de la fertilidad [8].…”

Section: Introductionunclassified

See 1 more Smart Citation

Análisis cromosómico de ganado bovino puro y cruzado en el departamento Norte de Santander, Colombia

Munar

Gil

Mercado

2021

Rev. Fac. Cienc. Básicas

View full text Add to dashboard Cite

El manejo reproductivo adecuado es fundamental para alcanzar índices óptimos en un sistema de producción de bovinos. Sin embargo, existen numerosas causas para la disminución de la fertilidad, como las de orden genético, que pueden afectar gravemente el sistema. Entre estas, las anormalidades cromosómicas causan baja fertilidad, debido principalmente a muerte temprana de embriones desbalanceados. Por tanto, es importante diagnosticar la presencia de estas anomalías en las unidades de producción ganaderas. Con el propósito de establecer la posible existencia de bovinos portadores de anormalidades cromosómicas en fincas de Norte de Santander, se tomaron muestras de sangre periférica a 31 bovinos puros y cruzados, pertenecientes a quince municipios del departamento. Se realizaron cultivos de linfocitos de sangre periférica, para obtener cromosomas; se analizaron 25 células de cada animal, en metafase, en un microscopio de luz convencional, para determinar el número cromosómico y la presencia de anormalidades cromosómicas. Enseguida, se estableció que, en su totalidad, los individuos analizados presentaron el número cromosómico nor- mal para la especie (2n=60) y en ninguno se visualizaron anomalías en el cariotipo. Asimismo, se evidenció la presencia de un cromosoma Y de tipo acrocéntrico en todos los machos analizados, cromosoma característico de las razas cebuinas. Este resultado confirma el tipo racial prevaleciente en Norte de Santander: cruces Bos taurus x Bos indicus.

show abstract

Section: Resultados Y Discusiónunclassified

Section: Introductionunclassified

Análisis cromosómico de ganado bovino puro y cruzado en el departamento Norte de Santander, Colombia

Munar

Gil

Mercado

2021

Rev. Fac. Cienc. Básicas

View full text Add to dashboard Cite

show abstract

“…If an animal karyotype has RT 1/29, the chromosome set consists of 59 chromosomes (2n = 59) and routine staining allows identifying a large submetacentric chromosome on the preparation, in addition to sex chromosomes. Different authors described over 50 types of Robertsonian translocations in bovine cattle with different autosomes, but with more frequent involvement of chromosomes from pairs 1, 9, 14, 16, 21, 27, and 29 into structural transformations [13][14][15].…”

Section: Results Of Studiesmentioning

confidence: 99%

Chromosomal anomalies in dairy cattle as reasons of impaired fertility

Dzitsiuk¹,

Tіpіlo²

2019

Agric. sci. pract.

View full text Add to dashboard Cite

Aim. The evaluation of animals for the presence of chromosomal anomalies is one of the main tasks of prac- tical selection, aimed at detecting undesired chromosomal anomalies in early age, which may have negative impact on the reproductive and productive capability of cows and lead to considerable economic losses. The aim of the work is a cytogenetic analysis of the chromosome set in cows of Ukrainian Red-and-Motley dairy cattle breed, which will allow assuming a decrease in reproductive functions with chromosomal aberrations. Methods. We examined 53 cows of the Ukrainian Red-and-Motley dairy cattle breed in SE Research Farm Khrystynivske, IABG named after M.V. Zubets, NAAS. The investigation of chromosomal anomalies involved 72-h cultivation of lymphocytes from the peripheral blood of animals using the common methods. During a routine analysis the preparations were stained with 2 % Giemsa staining solution. The induction of G-bands for differential staining of chromosomes was conducted using 0.25 % solution of trypsin. The processing of study results was performed with Microsoft Excel software package. Results. The investigations in the aberration spectrum detected aneuploid and polyploid cells, breaks and fragments of chromosomes, premature chromo- some disjunction in mitosis and translocation. The total number of aberrant cells in cows with decreased fertil- ity was 14.69 ± 0.56 %, the number of aberrations per one investigated cell was 0.144, which was almost twice reliably (Р < 0.999) exceeding the values of similar features for cows which did not have problems with repro- duction. GTG-banding method was used to detect a new RT 13/23 Robertsonian translocation. Conclusions. The cytogenetic analysis of chromosome set of Ukrainian Red-and-Motley dairy breed cows allows assuming the connection between a decrease in the fertility of cows and chromosomal instability. A routine screening of dairy cows allows both evaluating the karyotype saturation with undesired chromosomal aberrations and using the obtained results to forecast the reproductive ability of an animal in the early age.

show abstract

“…Based on the presence of a chromosomal aberration, a candidate bull can be identified and culled from a breeding program. For local Indonesian cattle, as well as other local animals, we proposed to use this test as a new criterion of selection to of candidate sires, especially for AI commercial sperm production, (Ciptadi et al 2017). Ahmad et al (2004) mentioned that screening of breeding bulls of different breeds through karyotyping is important, especially bulls maintained at semen production units or Artificial Insemination Centers.…”

Section: Discussionmentioning

confidence: 99%

“…Yamanaka (1977) suggested that in order to identify each chromosome for karyotype analysis in cattle, the number, intensity, width, and disposition of each band, as well as size of chromosomes should be considered. However, Ciptadi et al (2017) suggested that in developing countries, Indonesia especially, where AI implementation has been started, at national or state levels especially in Artificial Insemination Center, it is necessary to introduce this karyotyping in selection programs, for chromosomal control of breeding males. Karyotyping is one amongst several culling parameters and could be used as an indicator of genetically normal bulls, because each can produce a large number of offspring per year/bull.…”

Section: Discussionmentioning

confidence: 99%

Short Communication: The normal karyotyping result of Indonesian native breed bull qualified for artificial insemination

et al. 2017

Self Cite

View full text Add to dashboard Cite

Ciptadi G, Ihsan MN, Nurgiartiningsih VMA, Ardyah IP, Mudawamah M. 2017. Short Communication: The normal karyotyping result of Indonesian native breed bull qualified for artificial insemination. Biodiversitas 18: 1462-1467. Bali and Madura cattle are important native breed in Indonesia, both descendants of wild banteng (Bos javanicus). These breeds are categorized as a small type beef cattle, and are indigenous, well adapted and, have high resistance to diseases. The aims of this research were to analyze dendrograms of these breed and karyotype breeding bull selected for Artificial Insemination (AI). The result showed that the 2n diploid number of chromosome of all cattle was 60 and they were considered normal, based on the number and structure of chromosomes. Fiftyeight acrocentric autosomes and 2 sex chromosome were observed, including a submetacentric X-large chromosome submetacentric and a small Y chromosome. Dendrogram analysis showed that Bali and Madura cattle were closely related and derived more from their ancestor wild banteng than from Java cattle. It is recommended to perform a chromosomal investigation of breeding bulls especially those used for freezing semen production and AI implementation.

show abstract

The Comparison of Chromosome Analysis Result by Manual and Software Cytovision Image Analysis Using Simple G-Banding

Cited by 5 publications

References 8 publications

Análisis cromosómico de ganado bovino puro y cruzado en el departamento Norte de Santander, Colombia

Análisis cromosómico de ganado bovino puro y cruzado en el departamento Norte de Santander, Colombia

Chromosomal anomalies in dairy cattle as reasons of impaired fertility

Short Communication: The normal karyotyping result of Indonesian native breed bull qualified for artificial insemination

Contact Info

Product

Resources

About