The comparative toxicity of xanthones and tannins in mangosteen (Garcinia mangostana Linn.) pericarp extract against BHK-21 fibroblast cell culture

Puteri, Fikarini Hadi; Widjaja, Jennifer; Cahyani, Febriastuti; Mooduto, Latief; Wahjuningrum, Dian Agustin

doi:10.4103/ccd.ccd_579_18

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…74 By comparing the WC in CSONE and CTNE after 48 h, the cell migration and density showed an improvement in the CTNE-treated cell group (Figure S4). This investigation of the effect on wound healing was limited to the WC assay using a non-human mammalian fibroblast cell line (BSR); 32 It is, however, commonly used in such work 75,76 and suggests further characterisation of relevant human fibroblasts and suitable models could be warranted.…”

Section: Discussionmentioning

confidence: 99%

Formulation and Biomedical Activity of Oil-in-Water Nanoemulsion Combining Tinospora smilacina Water Extract and Calophyllum inophyllum Seeds Oil

Saki¹,

Murthy²,

Wang³

et al. 2023

CCID

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Introduction: Tinospora smilacina is a native plant used in traditional medicine by First Nations peoples in Australia to treat inflammation. In our previous study, an optimised Calophyllum inophyllum seed oil (CSO) nanoemulsion (NE) showed improved biomedical activities such as antimicrobial, antioxidant activity, cell viability and in vitro wound healing efficacy compared to CSO. Methods: In this study, a stable NE formulation combining T. smilacina water extract (TSWE) and CSO in a nanoemulsion (CTNE) was prepared to integrate the bioactive compounds in both native plants and improve wound healing efficacy. D-optimal mixture design was used to optimise the physicochemical characteristics of the CTNE, including droplet size and polydispersity index (PDI). Cell viability and in vitro wound healing studies were done in the presence of CTNE, TSWE and CSO against a clone of baby hamster kidney fibroblasts (BHK-21 cell clone BSR-T7/5). Results:The optimised CTNE had a 24 ± 5 nm particle size and 0.21± 0.02 PDI value and was stable after four weeks each at 4 °C and room temperature. According to the results, incorporating TSWE into CTNE improved its antioxidant activity, cell viability, and ability to promote wound healing. The study also revealed that TSWE has >6% higher antioxidant activity than CSO. While CTNE did not significantly impact mammalian cell viability, it exhibited wound-healing properties in the BSR cell line during in vitro testing. These findings suggest that adding TSWE may enhance CTNE's potential as a wound-healing treatment. Conclusion: This is the first study demonstrating NE formulation in which two different plant extracts were used in the aqueous and oil phases with improved biomedical activities.

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Section: Discussionmentioning

confidence: 99%

Formulation and Biomedical Activity of Oil-in-Water Nanoemulsion Combining Tinospora smilacina Water Extract and Calophyllum inophyllum Seeds Oil

Saki¹,

Murthy²,

Wang³

et al. 2023

CCID

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“…36 The large of tannins in the extract may cause liver and kidney damage. 37,38 The V group's histopathological analysis of the kidneys (Figure 1) dose showed moderately severe damage. This is thought to be due to the constituents in the extract increasing the permeability of the lipid bilayer of red blood cells, which can cause haemolysis of these cells.…”

Section: Organ Histopathologymentioning

confidence: 98%

A Subchronic Toxicity Test of Dillenia ochreata Leaves Extract on Wistar rats

2023

TJNPR

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Optimisation of Calophyllum inophyllum seed oil nanoemulsion as a potential wound healing agent

Saki

Murthy

Khandanlou

et al. 2022

BMC Complement Med Ther

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Background Efficient delivery systems of Calophyllum inophyllum seed oil (CSO) in the form of nanoemulsion were optimised to enhance its stability and ensure its therapeutic efficiency as a potential agent for various biomedical applications. Method Response Surface Methodology (RSM) was used to determine the effects of independent variables (oil, surfactant, water percentage and homogenisation time) on physicochemical characteristics, including droplet size, polydispersity index and turbidity. Results The optimised CSO nanoemulsion (CSONE) has a 46.68 nm particle size, 0.15 Polydispersity index value and 1.16 turbidity. After 4 weeks of storage at 5 ± 1 °C and 25 ± 1 °C, the CSONE was physically stable. The optimised CSO nanoemulsion showed enhancement in cell viability and wound healing in baby hamster kidney a clone BHK-21 (BSR) cells as compared to the CSO. The wound healing property of CSONE was higher than CSO. Conclusion Thus, our in vitro wound healing results demonstrated that CSO in the nanoemulsion form can promote wound healing by enhancing the proliferation and migration of epidermal cells. Graphical Abstract The coarse emulsion of Calophyllum inophyllum seed oil nano emulsion was prepared using high shear homogeniser techniques. The optimised CSONE with the droplet size of 46.68 nm was prepared from a mixture of CSO, Tween 80, and high pure water (HPW), then used for the biological investigation. The in vitro cell monolayer scratch assay revealed that CSONE in the lowest concentration of CSO resulted in 100% wound closure after 48 hrs. The optimised CSO nanoemulsion was found to be a promising and effective approach in the treatment of wounds by boosting the proliferation and migration of epidermal cells.

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The comparative toxicity of xanthones and tannins in mangosteen (Garcinia mangostana Linn.) pericarp extract against BHK-21 fibroblast cell culture

Cited by 3 publications

References 12 publications

Formulation and Biomedical Activity of Oil-in-Water Nanoemulsion Combining Tinospora smilacina Water Extract and Calophyllum inophyllum Seeds Oil

Formulation and Biomedical Activity of Oil-in-Water Nanoemulsion Combining Tinospora smilacina Water Extract and Calophyllum inophyllum Seeds Oil

A Subchronic Toxicity Test of Dillenia ochreata Leaves Extract on Wistar rats

Optimisation of Calophyllum inophyllum seed oil nanoemulsion as a potential wound healing agent

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