The comparative potencies of the crystalline endotoxin of eight varieties of Bacillus thuringiensis to larvae of Pieris brassicae

Galowalia, M. M. S.; Gibson, Naomi; Wolf, Jan H. D.

doi:10.1016/0022-2011(73)90216-4

Cited by 7 publications

(2 citation statements)

References 15 publications

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“…S. litura has shown the ability to evolve resistance to virtually all synthetic insecticides used against it (Ramakrishnan et al 1983;Singh & Singh 1998). Various synthetic insecticides in combination with biopesticides have been used in due course, to control various pests including S. litura under Integrated Pest Management strategies (Galowalia et al 1973;Deshpande & Ramakrishnan 1982;Dabi et al 1988;Gupta & Rana 1991;Mathur et al 1994;Rao & Krishnayya 1996;Kalia & Joshi 1997;Puntambekar et al 1997;Chandra et al 1998;Gloriana et al 2000). Different Bacillus thuringiensis subspecies are now being continuously tested in lab and field conditions for the control of S. litura (Amonkar et al 1985;Devi et al 1996;Kamala Jayanthi & Padmavathamma 1997).…”

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Relative efficacy of two subspecies ofBacillus thuringiensis, available as commercial preparations in market, on different stages of a lepidopteran pest,Spodoptera litura(Fabricius)

Pandey

Joshi

Tiwari

2009

Archives Of Phytopathology And Plant Protection

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The present study compares the efficacy of two different subspecies of Bacillus thuringiensis viz. aizawai and kurstaki, available as commercial preparations in market against different stages of Spodoptera litura. Leaf-dip bioassay was done to calculate the LC 50 values of both the preparations, while microbiological analysis and total protein content estimation were also performed to co-relate the results. B. thuringiensis aizawai was found to be more effective (LC 50 values being 0.505, 1.121, 5.009, 6.706 for 1 st , 2 nd , 3 rd , and 4 th instar, respectively) than kurstaki (LC 50 values being 1.121, 3.974, 6.897, 9.471 for 1 st , 2 nd , 3 rd , and 4 th instar, respectively). The efficacy was found to depend upon the number of viable spores in a particular preparation as well as on the total protein content of that particular subspecies. The preparation containing B.t.a was more effective because its viable spore counts as well as total protein content values (at different time intervals) were higher as compared to the preparation containing B.t.k. The growth rates for both the subspecies were almost the same, which didn't seem to affect their efficacy on S. litura.

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confidence: 99%

Relative efficacy of two subspecies ofBacillus thuringiensis, available as commercial preparations in market, on different stages of a lepidopteran pest,Spodoptera litura(Fabricius)

Pandey

Joshi

Tiwari

2009

Archives Of Phytopathology And Plant Protection

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“…Although the strains of B. thuringiensis form a well-defined group which is closely related biochemically (de Barjac & Bonnefoi, 1968) and genetically (Somerville & Jones, 1972) to Bacillus cereus, there are strain differences in immunological properties (Krywienczyk & Angus, 1966;Pendleton & Morrison, 1967) and activities towards different target species of Lepidoptera (Galowalia et al, 1973). It is not known, however, whether there are major strain variations in the number or nature of the polypeptide components of the bipyramidal inclusions characteristic of the B. thuringiensis group.…”

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confidence: 99%

Purification of the Insecticidal Toxin in Crystals of Bacillus thuringiensis

1980

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Crystals were purified from four serotypes of the insect pathogen Bacillus thuringiensis. Crystals from these serotypes were similar in amino acid and N-terminal analyses, but differed in their toxicity to two species of Lepidoptera and in their immunological properties. Toxic polypeptides were obtained following trypsin digestion of solutions of the crystals. In two strains (serotypes 3 and 9) this fraction contained only one polypeptide. Similar results were obtained when dissolved crystals were digested with other proteolytic enzymes or with gut contents from Pieris brassicae. The trypsin-resistant polypeptide was further purified by gel and ion-exchange chromatograhy and had a molecular weight of about 70,000, estimated by gel chromatogrpahy and gel electrophoresis. No evidence was obtained for a toxin of lower molecular weight. This purified toxin accounted for most, if not all, of the toxic activity originally present in the crystal solution and was active by injection and ingestion. The purified toxic fraction from serotype 1 appeared to contain two polypeptides, one of which corresponded to that found with serotypes 3 and 9. There were no major differences in the composition of crystals from different serotypes of B. thuringiensis and it is concluded that the trypsin-resistant polypeptide represents the active insecticidal toxin of the crystal.

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Nature of Pathogenic Process of Bacillus thuringiensis

Faust¹

1984

Pathogens of Invertebrates

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The comparative potencies of the crystalline endotoxin of eight varieties of Bacillus thuringiensis to larvae of Pieris brassicae

Cited by 7 publications

References 15 publications

Relative efficacy of two subspecies ofBacillus thuringiensis, available as commercial preparations in market, on different stages of a lepidopteran pest,Spodoptera litura(Fabricius)

Relative efficacy of two subspecies ofBacillus thuringiensis, available as commercial preparations in market, on different stages of a lepidopteran pest,Spodoptera litura(Fabricius)

Purification of the Insecticidal Toxin in Crystals of Bacillus thuringiensis

Nature of Pathogenic Process of Bacillus thuringiensis

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