The comparative influence of substituted phenols (especially chlorophenols) on yast cells assayed by elecro-rotation and other methods

Wm, Arnold; Zimmermann, U.; Pauli, W.; M, Benzing; Niehrs, Christof; Ahlers, J.

doi:10.1016/0005-2736(88)90277-5

Cited by 27 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The yeast used was Saccharomyces cerevisiae (strain RXII) obtained from the Department of Biophysics, Free University, Berlin. Electrorotation characteristics for this strain of yeast have been obtained previously by research groups in Germany and Bangor (Arnold et al 1988a, b, 1989, Hölzel and Lamprecht 1992, Huang et al 1992, Pauli et al 1993. The cells were grown in an aqueous medium (SYP) containing 5% sucrose (Oxoid), 0.5% yeast extract (Oxoid) and 0.5% peptone (Oxoid) at 30 • C in an incubator-shaker at 200 rpm.…”

Section: Yeast Cellsmentioning

confidence: 99%

“…The resulting ROT spectrum can be characterized by the frequencies and magnitudes of the anti-field and co-field rotation peaks, and by the crossover frequency marking the transition between the two senses of rotation. Changes in the physicochemical properties and morphology of the cell membrane can produce significant changes in the ROT spectrum, especially with respect to the anti-field rotation peak and the cross-over frequency (Arnold et al 1986, 1988a, b, Pauli et al 1993, Wang et al 1994, Sukhorukov and Zimmermann 1996.…”

Section: Introductionmentioning

confidence: 99%

“…The dielectric and electrorotation properties of S. cerevisiae have also been extensively investigated. Such studies include the effects of chemical exposure (Lopez et al 1984, 1985, Geier et al 1987, Arnold et al 1988a, b, Stoicheva et al 1989, Pauli et al 1993, Zhou et al 1996, differences between viable and non-viable yeast (Crane and Pohl 1968, Huang et al 1992 and changes in dielectric properties during batch culture (Arnold et al 1989, Kell et al 1990.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Automatic cell electrorotation measurements: studies of the biological effects of low-frequency magnetic fields and of heat shock

1998

View full text Add to dashboard Cite

A computer-aided automatic imaging technique has been developed for measuring the electrorotation spectra of up to 256 particles at the same time. This offers advantages over the conventional manual method, especially when rapidly acquired statistical data are necessary in investigations of the response of cells or test beads to chemical exposure, for example. We have applied this technique to investigate the biological effects of heat shock and low-frequency EM fields reported by others for yeast cells. Although heat shock effects were observed, no changes of the electrorotational behaviour could be detected after exposing the cells to 50 Hz, 8 and 80 microT fields. Although this does not rule out the possibility that the cells were influenced by the magnetic fields, it does limit the number of possible physicochemical changes that might have occurred to their cell walls and membranes.

show abstract

Section: Yeast Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Automatic cell electrorotation measurements: studies of the biological effects of low-frequency magnetic fields and of heat shock

1998

View full text Add to dashboard Cite

show abstract

Evaluation of Phenolic Compound Toxicity Using a Bioluminescent Assay with the Fungus Gerronema viridilucens

Ventura

Mendes

Oliveira

et al. 2020

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Basidiomycetes (phylum Basidiomycota) are filamentous fungi characterized by the exogenous formation of spores on a club‐shaped cell called a basidium that are often formed on complex fruiting bodies (mushrooms). Many basidiomycetes serve an important role in recycling lignocellulosic material to higher trophic levels, and some show symbiotic relationships with plants. All known bioluminescent fungi are mushroom‐forming basidiomycetes in the order Agaricales. Hence, the disruption of the basidiomycete community can entirely compromise the carbon cycle in nature from fungi to higher trophic levels. The fungus Gerronema viridilucens was used in the present study to investigate the toxicity of a phenolic compound series based on the inhibition of its bioluminescence. The median effect concentration (EC50) obtained from curves of bioluminescence inhibition versus log [phenolic compound] showed that 2,4,6‐trichlorophenol was the most toxic compound in the series. The log EC50 values of all phenolic compounds were then used for the prediction of their toxicity. The univariate correlation of log EC50 values obtained from 6 different phenolic compounds was stronger with the dissociation constant (pKa) than with 1‐octanol/water partition coefficient (KOW). Nevertheless, the toxicity can be better predicted by using both parameters, suggesting that the phenol‐driven uncoupling of fungus mitochondrial adenosine triphosphate synthesis is the origin of phenolic compound toxicity to the test fungus. Environ Toxicol Chem 2020;39:1558–1565. © 2020 SETAC

show abstract

Detecting membrane impairment caused by xenobiotics

Pauli

Berger

Köhler

et al. 1993

Environ. Toxicol. Water Qual.

View full text Add to dashboard Cite

The toxicity of 14 substituted anilines and 15 aliphatic alcohols was assessed by the electrorotation of yeast cells. Depending on the applied frequency, control cells exhibit both anti‐ and cofield rotation. From this spectrum, a frequency was selected at which untreated cells do not rotate, but impaired cells rotate with the field. The experimental spectra are fully in accord with theoretical models of the rotational response, calculated on the basis of changes in membrane permeability. High correlations exist between rotational data and physiological and biochemical end points (growth rate, plasma membrane ATPase, purine transport of yeast cells, and antihemolytic assays with human erythrocytes). A Quantitative Structure‐Activity Relationship analysis was made, whereby rotation data from 23 phenols were included. The effects of all chemicals on cell rotation could be predicted by their lipophilicity. Some residuals and deviations could be accounted for by the inclusion of molecular weight or connectivity in the analysis. 4‐Nitrophenol was an outlier, having an effect at least one order of magnitude higher than predicted, suggesting a specific toxic mechanism. Based on lipophilicity and rotation data, the antihemolytic effect on erythrocytes could be accurately estimated. © 1993 John Wiley & Sons, Inc.

show abstract

The comparative influence of substituted phenols (especially chlorophenols) on yast cells assayed by elecro-rotation and other methods

Cited by 27 publications

References 20 publications

Automatic cell electrorotation measurements: studies of the biological effects of low-frequency magnetic fields and of heat shock

Automatic cell electrorotation measurements: studies of the biological effects of low-frequency magnetic fields and of heat shock

Evaluation of Phenolic Compound Toxicity Using a Bioluminescent Assay with the Fungus Gerronema viridilucens

Detecting membrane impairment caused by xenobiotics

Contact Info

Product

Resources

About