The compaction of mouse heterochromatin as studied by nuclease digestion

Horvath, Peter J.; Hörz, Wolfram

doi:10.1016/0014-5793(81)80542-x

Cited by 13 publications

(9 citation statements)

References 21 publications

(23 reference statements)

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“…This idea will be developed further in a separate paper. Packaging of DNA repeats into dense heterochromatin has been demonstrated by various approaches [45,63-73]. This notion is also consistent with two other observations: (1) the spatial clustering of dispersed repeats in chromosomes [23-25]; and (2) tandem-repeat-based formation of densely packed chromomeres of chicken lampbrush chromosomes [74].…”

Section: Rp In Chromosomal Organizationsupporting

confidence: 82%

“…Cations may also facilitate RP by stabilizing repeat Z-DNA [44]. Indeed, divalent cations are the key factors that determine the stability of DNA bundles formed in vitro [21] and repeat-formed heterochromatin [45]. Additionally, RPs may also be regulated by macromolecules, such as RNAs, ‘scaffold’ proteins (e.g., TOPO II and SMC), and repeat DNA-binding proteins [46-52], by their interactions with repeats.…”

Section: Repeat Pairing (Rp)mentioning

confidence: 99%

“…For example, some satellite tandem repeats may form very stable RAs, and thus would be frequently found in heterochromatic regions [64,65]. RA stability is presumably regulated by the ionic environment and the cell state [45,71-73]. Therefore, it is unlikely that all repeats are in RAs during any given cell state.…”

Section: Rp In Chromosomal Organizationmentioning

confidence: 99%

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Chromatin Organization by Repetitive Elements (CORE): A Genomic Principle for the Higher-Order Structure of Chromosomes

Tang¹

2011

Genes

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Eukaryotic genomes contain a large amount of DNA repeats (also known as repetitive DNA, repetitive elements, and repetitive sequences). Here, I propose a role of repetitive DNA in the formation of higher-order structures of chromosomes. The central idea of this theory is that chromatin regions with repetitive sequences pair with regions harboring homologous repeats and that such somatic repeat pairing (RP) assembles repetitive DNA chromatin into compact chromosomal domains that specify chromatin folding in a site-directed manner. According to this theory, DNA repeats are not randomly distributed in the genome. Instead, they form a core framework that coordinates the architecture of chromosomes. In contrast to the viewpoint that DNA repeats are genomic ‘junk’, this theory advocates that repetitive sequences are chromatin organizer modules that determine chromatin-chromatin contact points within chromosomes. This novel concept, if correct, would suggest that DNA repeats in the linear genome encode a blueprint for higher-order chromosomal organization.

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Section: Rp In Chromosomal Organizationsupporting

confidence: 82%

Section: Repeat Pairing (Rp)mentioning

confidence: 99%

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Chromatin Organization by Repetitive Elements (CORE): A Genomic Principle for the Higher-Order Structure of Chromosomes

Tang¹

2011

Genes

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“…It was speculated that Mg 2+ is a trigger of the condensation-decondensation transition of chromosomes during mitosis (Jerzmanowski and Staron, 1980). Cations are known to regulate the compact state of tandem repeats in heterochromatins (Horvath and Hörz, 1981; Zhang and Horz, 1982). The Ca 2+ concentration is higher on the AT-rich axis of mitotic chromosomes, probably binding to the minor groove of the AT-rich sequence (Strick et al, 2001a).…”

Section: Cation-regulated Rp During Mitosismentioning

confidence: 99%

A repetitive DNA-directed program of chromosome packaging during mitosis

Tang

2016

Journal of Genetics and Genomics

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“…We have previously shown for satellite chromatin from mouse liver nuclei that its characteristically condensed state is highly sensitive to the ionic environment (6). These studies have now been extended and a purification scheme for mouse satellite chromatin was developed.…”

Section: Introductionmentioning

confidence: 99%

Analysis of highly purified satellite DNA containing chromatin from the mouse

Zhang¹,

Hörz²

1982

Nucl Acids Res

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A purification scheme for satellite DNA containing chromatin from mouse liver has been developed. It is based on the highly condensed state of the satellite chromatin and also takes advantage of its resistance to digestion by certain restriction nucleases. Nuclei are first treated with micrococcal nuclease and the satellite chromatin enriched 3-5 fold by extraction of the digested nuclei under appropriate conditions. Further purification is achieved by digestion of the chromatin with a restriction nuclease that leaves satellite DNA largely intact but degrades non-satellite DNA extensively. In subsequent sucrose gradient centrifugation the rapidly sedimenting chromatin contains more than 70% satellite DNA. This material has the same histone composition as bulk chromatin. No significant differences were detected in an analysis of minor histone variants. Nonhistone proteins are present only in very low amounts in the satellite chromatin fraction, notably the HMG proteins are strongly depleted.

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The compaction of mouse heterochromatin as studied by nuclease digestion

Cited by 13 publications

References 21 publications

Chromatin Organization by Repetitive Elements (CORE): A Genomic Principle for the Higher-Order Structure of Chromosomes

Chromatin Organization by Repetitive Elements (CORE): A Genomic Principle for the Higher-Order Structure of Chromosomes

A repetitive DNA-directed program of chromosome packaging during mitosis

Analysis of highly purified satellite DNA containing chromatin from the mouse

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