A repetitive DNA-directed program of chromosome packaging during mitosis

Tang, Shao-Jun

doi:10.1016/j.jgg.2016.04.003

Cited by 2 publications

(2 citation statements)

References 44 publications

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“…The important point in this hypothesis is that the DNA is the main actor in the phase separation, which is in contrast to the LLPS mechanisms driven by protein interactions and/or protein-RNA interactions. In other words, repetitive DNA sequences may function as key chromosomal packaging modules [121,122]. Furthermore, a recent study using Hi-C (high-throughput chromosome conformation capture) data suggested the inter-chromosomal co-localization of several repetitive DNA sequences, especially those of the SINE (short interspersed element) family, in Drosophila , mouse, and human nuclei [123].…”

Section: Mg2+ and Repetitive Dna Folding And Phase Separationmentioning

confidence: 99%

New Aspects of Magnesium Function: A Key Regulator in Nucleosome Self-Assembly, Chromatin Folding and Phase Separation

Ohyama

2019

IJMS

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Metal cations are associated with many biological processes. The effects of these cations on nucleic acids and chromatin were extensively studied in the early stages of nucleic acid and chromatin research. The results revealed that some monovalent and divalent metal cations, including Mg2+, profoundly affect the conformations and stabilities of nucleic acids, the folding of chromatin fibers, and the extent of chromosome condensation. Apart from these effects, there have only been a few reports on the functions of these cations. In 2007 and 2013, however, Mg2+-implicated novel phenomena were found: Mg2+ facilitates or enables both self-assembly of identical double-stranded (ds) DNA molecules and self-assembly of identical nucleosomes in vitro. These phenomena may be deeply implicated in the heterochromatin domain formation and chromatin-based phase separation. Furthermore, a recent study showed that elevation of the intranuclear Mg2+ concentration causes unusual differentiation of mouse ES (embryonic stem) cells. All of these phenomena seem to be closely related to one another. Mg2+ seems to be a key regulator of chromatin dynamics and chromatin-based biological processes.

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Section: Mg2+ and Repetitive Dna Folding And Phase Separationmentioning

confidence: 99%

New Aspects of Magnesium Function: A Key Regulator in Nucleosome Self-Assembly, Chromatin Folding and Phase Separation

Ohyama

2019

IJMS

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“…The intracellular ionic environment is very dynamic and changes according to different cell states. As discussed previously, the ionic strength of various cations such as Ca 2+ and Mg 2+ may be crucial in regulating RA-based chromatin folding [ 19 ]. I expect that the cell state-based changes of ionic strength would play an important role in regulating the separation of repeats.…”

Section: Regulation Of the Phase Separation Of Repetitive Dna Elemmentioning

confidence: 99%

Potential Role of Phase Separation of Repetitive DNA in Chromosomal Organization

Tang

2017

Genes

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The basic principles of chromosomal organization in eukaryotic cells remain elusive. Current mainstream research efforts largely concentrate on searching for critical packaging proteins involved in organizing chromosomes. I have taken a different perspective, by considering the role of genomic information in chromatins. In particular, I put forward the concept that repetitive DNA elements are key chromosomal packaging modules, and their intrinsic property of homology-based interaction can drive chromatin folding. Many repetitive DNA families have high copy numbers and clustered distribution patterns in the linear genomes. These features may facilitate the interactions among members in the same repeat families. In this paper, the potential liquid–liquid phase transition of repetitive DNAs that is induced by their extensive interaction in chromosomes will be considered. I propose that the interaction among repetitive DNAs may lead to phase separation of interacting repetitive DNAs from bulk chromatins. Phase separation of repetitive DNA may provide a physical mechanism that drives rapid massive changes of chromosomal conformation.

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A repetitive DNA-directed program of chromosome packaging during mitosis

Cited by 2 publications

References 44 publications

New Aspects of Magnesium Function: A Key Regulator in Nucleosome Self-Assembly, Chromatin Folding and Phase Separation

New Aspects of Magnesium Function: A Key Regulator in Nucleosome Self-Assembly, Chromatin Folding and Phase Separation

Potential Role of Phase Separation of Repetitive DNA in Chromosomal Organization

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