The Combined Utility of HBME-1 and Galectin-3 Immunohistochemistry and BRAF V600E Mutations in the Diagnosis of Papillary Thyroid Carcinoma

Ramkumar, S.; Sivanandham, Shanthakumari

doi:10.7759/cureus.20339

Cited by 6 publications

(7 citation statements)

References 38 publications

(41 reference statements)

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“…However, none of these antibodies presented enough sensitivity and specificity to be used in routine practice. 78 , 79 , 80 , 81 , 82 , 83 In recent years, a specific monoclonal antibody (Ve1) against the mutated V600E BRAF protein began to be used for differential diagnosis and patient management. Few reports on the cytological application-assisted application of BRAF Ve1 antibody suggest that immunohistochemistry can be performed more reliably in cell block preparations, and false-positive results in direct smears may limit the benefit; thus, caution should be exercised in interpretation.…”

Section: Ancillary Techniques Used For Differential Diagnosis Of Foll...mentioning

confidence: 99%

Follicular Growth Pattern Disease on Thyroid Fine-needle Aspiration Biopsy

2022

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Thyroid nodules are a common worldwide health problem and a diagnostic challenge for clinicians and cytopathologists. Follicular growth pattern constitutes the majority of thyroid lesions. Thyroid nodules can be neoplastic or non-neoplastic, and neoplastic nodules can be classified as benign, malignant, or gray zone. Gray zone lesions include different benign and malignant entities that might be resulted in unnecessary thyroidectomies with risk of morbidity and higher health care costs. Depending on the cellularity, most cases might fall into the follicular neoplasia (FN)/ suspicious for FN (SFN) category or follicular lesion of undetermined significance (FLUS) in The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC). Pathologists must be aware of the relationship between this diagnostic category and follow-up patient management and avoid over-diagnosing by mastering the diagnostic criteria.

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Section: Ancillary Techniques Used For Differential Diagnosis Of Foll...mentioning

confidence: 99%

Follicular Growth Pattern Disease on Thyroid Fine-needle Aspiration Biopsy

2022

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“…Din datele obţinute reiese că reacţia imunohistochimică la peroxidaza tiroidiană poate să prezinte un procedeu de diagnostic, suplimentar la cel histopatologic. (9,10) Expresia tireoglobulinei Tireoglobulina, care rezultă din cuplarea intracitoplasmatică a globulinei cu T 3 şi T 4 şi care este componentul principal alcoloidului folicular, a fost investigată imunohistochimic în 6 adenoame foliculare, dintre care 4 au fost atipice, şi în 25 carcinoame, dintre care 15 au fost de tip folicular şi 10 -de tip papilar.…”

Section: Expresia Peroxidazei Tiroidieneunclassified

Personalized immunohistochemical particulars of thyroid cancer in children

Tibirna

Stancu²,

Bejenaru³

2022

BulMed

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The idea of the paper from the state scientific program under the name „Modern personalized surgery in the diagnosis and complex treatment of tumors in children”. In the first stage, the executors conducted statistical research on the incidence of thyroid cancer in children. During 40 years from 18549 cases of nodular thyroid pathology, 1078 cases were detected at children: 105 - (9.7%) with cancer, adenomas 534 (49.5%), toxic nodular goiter 213 (19.7%) , struma Hashimoto 226 (21%),: ratio girl : boys 6: 1. The most affected age is 9-16 years. The highest morbidity is in children aged from 12 to 15 years.The morphological structure of thyroid cancer in children (105 patients) was as follows: first - papillary cancer - 68.6%, the second follicular cancer 23.8%, the third medullary cancer - 5.7% and the fourth anaplastic cancer - 1.9%.The priority goal of the authors was personalized medicine, which involves selecting an appropriate treatment for the patient, depending on the volume of the primary tumor, the morphological type and the biological evolution of the tumor. In the diagnostic plan for the assessment of tumor aggression, the authors will propose immunohistochemical research in assessing the angioinvasive aggression of thyroid carcinomas in children. It is proposed to investigate the expression of thyroiglobulin, keratin and vimentin, peroxidase. Peroxidase expression was studied in 22 children. Thyroglobulin expression was studied in 31 children. And the expression of keratin and vimentin was studied in 16 children [2, 4-6].

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“…Interesting results came from the correlation between HBME-1 immunohistochemical expression and genetic mutations, with a trend noted in follicular carcinomas with peroxisome proliferator-activated receptor (PPAR) rearrangements that fail to show HBME-1 immunoreactivity in 59% of cases, in contrast to RAS mutated cases that are often positive (62% of cases) 28. This strict relation with the underlying genetic background of thyroid neoplasms culminates in PTC, in which a direct positive correlation between BRAFV600E mutation and HBME-1 expression has been noted 29…”

Section: Introduction and Historical Perspectivementioning

confidence: 99%

Lights on HBME-1: the elusive biomarker in thyroid cancer pathology

et al. 2022

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Among the different ancillary immunohistochemical tools that pathologists may employ in thyroid nodules, the so-called Hector Battifora’s ‘MEsothelioma’ 1 (HBME-1) staining is one of the most fascinating, since its real identity is currently unknown. In the present review, the different clinical applications of HBME-1 are analysed, with main emphasis on its role in thyroid pathology with overview on less impactful fields, such as haematopathology or mesothelial lesions. Different acceptable or good diagnostic performances were recorded for HBME-1 in thyroid pathology, being used in routine practice as one of the best tools to screen thyroid malignancy both in terms of sensitivity and specificity. From a speculative point of view, after many attempts to hunt the cryptic target antigen of this antibody, its identity still remains elusive. In this setting, the application of high-throughput technologies (mainly in situ proteomics) may be the exact route to improve the knowledge about the pathophysiology of HBME-1 and to finally unveil its true identity.

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The Combined Utility of HBME-1 and Galectin-3 Immunohistochemistry and BRAF V600E Mutations in the Diagnosis of Papillary Thyroid Carcinoma

Cited by 6 publications

References 38 publications

Follicular Growth Pattern Disease on Thyroid Fine-needle Aspiration Biopsy

Follicular Growth Pattern Disease on Thyroid Fine-needle Aspiration Biopsy

Personalized immunohistochemical particulars of thyroid cancer in children

Lights on HBME-1: the elusive biomarker in thyroid cancer pathology

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