The combined extraction of sage (Salvia officinalis L.): Ultrasound followed by supercritical CO2 extraction

Abstract

A wide spectrum of phytochemicals could be isolated from sage (Salvia officinalis L.) using different extraction or distillation technique: the supercritical fluid extraction (SFE), the volatiles compounds (monoterpenes and sesquiterpenes) isolation using hydrodistillation or higher molecular compounds with Soxhlet extraction or ultrasound-assisted extraction. The combination of ultrasound-assisted extraction followed by re-extraction of obtained extract with supercritical CO(2) was performed in this study. The goal of performed investigation was to concentrate diterpenes present in sage extract which are generally considered to be responsible for antioxidant activity of extracted compounds. The fractionation using the supercritical CO(2), and different combination of the ultrasound-assisted solvent extractions (water-ethanol mixture or only water) followed by supercritical CO(2) re-extraction of obtained extract or treated plant material were analyzed and compared. Based on the results of these investigations it could be proposed the best extraction procedure: the ultrasound pretreatment of plant material with distilled water and re-extraction of plant material (residue) using supercritical CO(2). That procedure gives two valuable products: the ultrasound extract which is rich in sugars and possess the immunomodulatory activity and supercritical extract which is rich in diterpenes and sesquiterpenes.

Supercritical Fluid Extraction of Plant Flavors and Fragrances

“…The combination of ultrasound-assisted extraction followed by re-extraction of obtained sage extract with SC CO 2 was also performed. This procedure gave two valuable products: the ultrasound extract which was rich in sugars and SFE extract which was rich in terpenoids [97]. The influence of process parameters, such CO 2 density and extraction time, on the composition of sage extracts was also studied.…”

Optimización de la extracción de antioxidantes de Salvia officinalis L. con CO2 supercrítico

“…El ACA es considerado el diterpeno con mayor actividad antioxidante entre los detectados en los extractos de salvia; además, la actividad antiinflamatoria de los extractos de salvia y romero se relaciona con el alto contenido de este componente (Peter, 2000); por esta razón, el ACA se considera el componente que le proporciona calidad a los extractos de romero y salvia y que, además, determina su precio. Glisic y colaboradores obtuvieron extractos con una alta proporción de diterpenos y sustancias semivolátiles (~40 % en peso) cuando sometieron el residuo de la extracción con agua asistida por ultrasonidos a extracción con CO 2 supercrítico (Glisic, et al, 2011). Aunque en ese trabajo no se realizó la evaluación de estos extractos, su composición hace esperar que estas fracciones poseen alta capacidad antioxidante.…”

Optimization of ultrasound-assisted extraction of phenolic compounds, antioxidants and rosmarinic acid from perilla leaves using response surface methodology

“…Several methods are available for extraction of phenolic compounds from plants, including solvent extraction, ultrasound-assisted extraction, microwave-assisted extraction, and supercritical fluid extraction (Glisic et al, 2011;Hossain et al, 2012;Caxambu et al, 2016). Of these methods, ultrasound-assisted extraction (UAE) technology has gained increasing popularity due to its advantageous properties, including high extraction efficiency, good reproducibility, low solvent consumption, speed, low cost, environmental friendliness and easy scale up for industrial applications (Tao et al, 2014;Cravotto et al, 2008;Vinatoru, 2001;Vuong et al, 2014).…”