The Combined Effect of Hydrogen Peroxide and Ultraviolet Irradiation on Bacterial Spores

Bayliss, Catherine E.; Waites, W. M.

doi:10.1111/j.1365-2672.1979.tb01753.x

Cited by 63 publications

(34 citation statements)

References 13 publications

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“…However, the form in which some of their data is presented is amenable to modelling by the techniques described in the Materials and Methods section. In Bayliss and Waites (1979b), data are presented of fractional survival of spores of B. subtilis 738 against exposure time for the combined u.v.-hydrogen peroxide treatment. The u.v.…”

Section: Discussionmentioning

confidence: 99%

The kinetics of Bacillus subtilis spore inactivation on filter paper by u.v. light and u.v. light in combination with hydrogen peroxide

Gardner

Shama

1998

J Appl Microbiol

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Inactivation of spores of Bacillus subtilis (ATCC 6633) on two different grades of cellulose filter paper (Whatman Grades 2 and 6), by ultraviolet light (u.v.), at an intensity of approximately 4·5 Wm−2 and at fluences of up to 2 × 103 Jm−2, and u.v. in the presence of hydrogen peroxide, is described in terms of multi‐target and single hit–single target kinetic expressions. Wet spores were inactivated at rates ranging from 6·7 to 10·6 higher than that of dry spores on both grades of filter paper. In addition, spore inactivation was up to 5·6 times more rapid on Grade 2 filter paper. Synergistic inactivation was seen to occur when spores were irradiated in the presence of 1% (w/v) hydrogen peroxide with rates up to 5·3 times higher than with treatment solely by u.v. The results obtained are discussed in general terms with particular reference to surface characteristics which might provide shielding to micro‐organisms from incident u.v. light.

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Section: Discussionmentioning

confidence: 99%

The kinetics of Bacillus subtilis spore inactivation on filter paper by u.v. light and u.v. light in combination with hydrogen peroxide

Gardner

Shama

1998

J Appl Microbiol

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“…The synergistic bactericidal effect of hydrogen peroxide and UV light was observed by Bayliss and Waites (1979). The mode of action is considered to be primarily through the generation of antimicrobial radicals from hydrogen peroxide via exposure to UV light.…”

Section: Currently Used Methodsmentioning

confidence: 99%

Extended shelf life milk—advances in technology

Rysstad¹,

Kolstad

2006

Int J of Dairy Tech

127

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“…Stannard et al (1985) também verificaram redução decimal de 2,63 em embalagens de polietileno expostas a 10 cm da lâmpada germicida, utilizando esporo de Bacillus subtilis como microrganismo teste. Bayliss & Waites (1979) demonstraram que, para o tempo de 30 s e a distância da fonte de 5,5 cm, a porcentagem de sobreviventes de Bacillus subtilis foi de 1,44.…”

Section: Resultsunclassified

Avaliação da eficiência da radiação ultravioleta na esterilização de embalagens plásticas

2008

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RESUMOO uso da radiação ultravioleta é um dos vários processos físicos que podem ser utilizados para a esterilização ou na higienização de superfícies que irão entrar em contato com alimentos. Objetivou-se, nesta pesquisa a avaliação da eficiência da radiação ultravioleta (UV) na esterilização de superfícies de embalagens para alimentos visando a redução da contaminação microbiológica. Tampas e filmes plásticos foram irradiados em uma fonte de ultravioleta emitindo comprimentos de ondas a 254 nm, no interior de uma câmara especialmente projetada para este estudo, através de um delineamento fatorial para testar as variáveis: tempo de exposição e intensidade de irradiação (distância da fonte). Avaliou-se o número de reduções decimais de esporos de Bacillus subtilis inoculados na superfície das embalagens. As reduções decimais obtidas não foram altas, variaram de 0,21 a 2,47, mas o uso da lâmpada UV foi altamente eficiente para tempo de exposição superior a 30 s e distância mínima de 20 cm da fonte, causando a redução total da carga microbiana inoculada. Termos para indexação:Radiação UV, esterilização, embalagens plásticas. ABSTRACTUltraviolet radiation is one of the major physical processes used to sterilize or to clean surfaces that will be in contact of foods. This research evaluated the efficiency of ultraviolet (UV) radiation to sterilize surfaces of plastic packaging materials based on the reduction of microbial counts. Plastic closures and films were irradiated by exposing them to lamps emitting UV radiation at 254 nm in an apparatus specially constructed for this study. Using an experimental design in order to investigate the effect of exposure time and distance from the source, the reduction number of Bacillus subtilis spores inoculated to the package surfaces were evaluated. The UV radiation efficiency was not high for short exposition time, presenting reduction number ranged from 0.21 to 2.47, but it was very efficient when exposed to more than 30 s at a distance of 20 cm.

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The Combined Effect of Hydrogen Peroxide and Ultraviolet Irradiation on Bacterial Spores

Cited by 63 publications

References 13 publications

The kinetics of Bacillus subtilis spore inactivation on filter paper by u.v. light and u.v. light in combination with hydrogen peroxide

The kinetics of Bacillus subtilis spore inactivation on filter paper by u.v. light and u.v. light in combination with hydrogen peroxide

Extended shelf life milk—advances in technology

Avaliação da eficiência da radiação ultravioleta na esterilização de embalagens plásticas

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