The combination of calcium ionophore A23187 and GM-CSF can safely salvage aged human unfertilized oocytes after ICSI

Economou, Konstantinos; Christopikou, Dimitra; Tsorva, E.; Davies, Sara; Mastrominas, Minas; Cazlaris, Haris; Koutsilieris, Michael; Angelogianni, Panagoula; Loutradis, Dimitris

doi:10.1007/s10815-016-0823-0

Cited by 15 publications

(20 citation statements)

References 67 publications

(82 reference statements)

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“…Such a situation was reported in other animal (19) but appears to not be a case in human oocytes. In the present report, aneuploid (not triploid) blastocysts were observed in both groups, which may originate from oocytes, not from treatments, as quite high rates of embryonic aneuploidy were observed in human IVF (26). Furthermore, it has been suggested that sperm DNA fragmentation may be high in globozoospermia (5).…”

Section: Discussionsupporting

confidence: 53%

“…The explanation for this is that normal sperm fertilization can induce multiple calcium oscillations (23), while calcium ionophore can induce only one calcium oscillation (22) and one calcium oscillation may be not enough to initialize oocyte activation events in some oocytes. Furthermore, oocyte activation is also affected by other factors, such as oocyte maturation status (10,(24)(25)(26). It has been found that aged oocytes are more easily activated by AOA, such as failed fertilized oocytes after ICSI or purposely aged oocytes (10,25,26).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, oocyte activation is also affected by other factors, such as oocyte maturation status (10,(24)(25)(26). It has been found that aged oocytes are more easily activated by AOA, such as failed fertilized oocytes after ICSI or purposely aged oocytes (10,25,26).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Human Oocyte Activation Between Round-Headed Sperm Injection Followed by Calcium Ionophore Treatment and Normal Sperm Injection in a Patient With Globozoospermia

Niu¹,

Ruan²,

Witz

et al. 2020

Front. Endocrinol.

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Fertilization failure is common in patients with round-headed sperm, a form of globozoospermia. Artificial oocyte activation is able to assist oocyte fertilization after sperm injection in these patients. Comparisons between oocyte fertilization with or without calcium ionophore have been reported in patients with round-headed sperm. However, no comparison has been reported between round-headed sperm injection followed by calcium ionophone activation and normal sperm injection. In this case report, half of oocytes from a patient were injected with her partner's round-headed sperm followed by calcium ionophore activation, and the other half of oocytes were injected with a donor sperm without calcium ionophore activation. The injected oocytes were cultured to examine fertilization, embryo development, and embryonic aneuploidies in the resulting blastocysts. The fertilization rate was lower in round-headed sperm injected oocytes (3/6) than that in donor sperm injected oocytes (5/6), but rates of blastocyst and aneuploidies were similar in the resulting embryos between the two groups. A euploid blastocyst resulted from round-headed sperm injection was transferred, and a healthy baby was delivered. These results indicate that calcium ionophore treatment can assist oocyte activation in patients with round-headed sperm, but its efficiency to activate oocytes is lower than that induced by a normal sperm injection. However, embryo development and chromosome integrity may not be affected by calcium ionophore treatment.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of Human Oocyte Activation Between Round-Headed Sperm Injection Followed by Calcium Ionophore Treatment and Normal Sperm Injection in a Patient With Globozoospermia

Niu¹,

Ruan²,

Witz

et al. 2020

Front. Endocrinol.

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“…One Level-I study showed no benefit to the euploidy rate (34.8% treatment vs. 33.3% controls) of developing embryos (65) (69). A single RCT that used unfertilized oocytes activated by the calcium ionophore A23187 with or without GM-CSF suggested improved activated embryo development rates and quality (66). However, as calcium ionophores are not used in regular clinical practice and are focused on abnormal fertilization events only, the generalizability is unclear and not clinically applicable.…”

Section: Granulocyte Colony-stimulating Factor (G-csf) and Granulocyte/macrophage Colony-stimulating Factor (Gm-csf)mentioning

confidence: 99%

The role of immunotherapy in in vitro fertilization: a guideline

Penzias¹,

Bendikson²,

Butts³

et al. 2018

Fertility and Sterility

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Adjuvant immunotherapy treatments in in vitro fertilization (IVF) aim to improve the outcome of assisted reproductive technology (ART) in both the general ART population as well as subgroups such as patients with recurrent miscarriage or implantation failure. The purpose of this guideline is to evaluate the role of immunomodulating therapy in ART. Unfortunately, many of the evaluated therapies lack robust evidence from well-designed adequately powered randomized controlled trials to support their use. Immunotherapies reviewed in the present document are either not associated with improved live-birth outcome in IVF or have been insufficiently studied to make definitive recommendations.

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“…Similarly, a healthy girl was born after ICSI of oocytes with frozen-thawed spermatozoa and subsequent treatment with strontium chloride (Kim et al 2012). Recently, the combination of Ca +2 ionophore A23187 and granulocyte-macrophage colony-stimulating factor (GM-CSF) found to salvage unfertilized oocytes after ICSI (Kyono et al 2008;Economou et al 2017). In conclusion, although AOA is increasingly applied in human assisted reproduction, it should not be considered as an established treatment and has to be applied with caution.…”

Section: Non-natural Activatorsmentioning

confidence: 99%

Artificial oocyte activation: physiological, pathophysiological and ethical aspects

Anifandis

Michopoulos

Daponte

et al. 2018

Systems Biology in Reproductive Medicine

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AOA: artificial oocyte activation; AOD: oocyte activation deficiency; Ca: Calcium; CAMKII: Ca/calmodulin-dependent protein kinase II; CICR: calcium-induced calcium-release; DAG: diacylglycerol; GM-CSF: granulocyte-macrophage colony-stimulating factor; ICSI: intra-cytoplasmic sperm injection; InsPR: inositol-trisphosphate receptor; IP: inositol 1,4,5-trisphosphate; IVF: in vitro fertilization; MAP: mitogen-activated protein; MII: metaphase II; NADP: nicotinic acid adenine dinucleotide phosphate; NO: nitric oxide; PAWP: post-acrosomal WW-binding domain protein; PIP: phosphatidylinositol 4,5-bisphosphate; PLC: phospholipase C; PLCζ: phospholipase C-zeta; SOAFs: spermatozoon-released oocyte-activating factors; Sr: strontium; TFF: total fertilization failure.

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The combination of calcium ionophore A23187 and GM-CSF can safely salvage aged human unfertilized oocytes after ICSI

Abstract: This study shows that activating post-ICSI aged human unfertilized oocytes with a combination of a calcium ionophore and a cytokine can produce good-morphology euploid blastocysts.

Cited by 15 publications

References 67 publications

Comparison of Human Oocyte Activation Between Round-Headed Sperm Injection Followed by Calcium Ionophore Treatment and Normal Sperm Injection in a Patient With Globozoospermia

Comparison of Human Oocyte Activation Between Round-Headed Sperm Injection Followed by Calcium Ionophore Treatment and Normal Sperm Injection in a Patient With Globozoospermia

The role of immunotherapy in in vitro fertilization: a guideline

Artificial oocyte activation: physiological, pathophysiological and ethical aspects

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