The Colonization of the Human Gut by Antibiotic Resistant <i>Escherichia coli</i> from Chickens

Linton, A. H.; Howe, Katherine; Bennett, PM; Richmond, M. H.; Whiteside, Eliza

doi:10.1111/j.1365-2672.1977.tb00773.x

Cited by 126 publications

(72 citation statements)

References 13 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Surprising results that was no difference between inhibition zones of methicillin-resistant and methicillin-susceptible strain may be due to the some component of essential oil caused bacterial cell damaged this result is in agreement with [44][45][46]. They found that essential oil caused gross membrane damaged and provoke whole cell lysis of eukaryotic.…”

Section: Discussionsupporting

confidence: 77%

Antimicrobial, Antiviral Activity and GC-MS Analysis of Essential Oil Extracted from Achillea fragrantissima Plant Growing In Sinai Peninsula, Egypt

Zeedan¹

2014

J Microb Biochem Technol

View full text Add to dashboard Cite

Medicinal plants are considered new resources for producing agents that could act as alternatives to antibiotics in treatment of antibiotic-resistant bacteria. The present study aimed to evaluate the efficacy of essential oil extracted from Achillea fragrantissima plant growing in Egypt for antimicrobial, antiviral activities and chemical composition analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). We also performed determination of essential oil antimicrobial activity by agar desk diffusion method and Minimal Inhibitory Concentration (MIC). Also, antiviral activity on ORF virus by pock reduction test was performed. It was reduced virus titer from 5.9 to 1.00 at 180 minutes. Detection of beta lactams resistant bacteria (Gram-positive bacteria Staphylococcus aureus, Staphylococcus epidermidis (MRSA) and Gram-negative bacteria Escherichia coli) by PCR with primers of mecA gene and Bela gene. The essential oil obtained by hydrodistillation was analyzed by GC-MS. The major components were found to be Santolina triene (1.97%), 2,5,5-trimethyl-3,6-heptadien-2-ol (8.23%) Eucalyptol 8.17, trans-2,7-Dimethyl-4,6octadien-2-ol (24.40%), 1,5-Heptadien-4-one-3,6-trimethyl (7.65%), Artemisia alcohol (3.49%), à Thujone (33.97%), Cissabinol (1.92%), Lavandulol (0.71%), 2-Octen-4-ol, 2-methyl (2.02%), 3-Cyclohexen1ol,4-methyl1 (1 methylethyl) (CAS) (2.15%), à terpineol ( 0.05% ), Estragole (0.71%) Lavandulyl acetate (0.49%), Sabinyl acetate (2.12 %), Germacrened (0.94%). Finally, our study proved that the essential oil has bactericidal effect on some bacterial resistant antibiotic (Gram-positive bacteria Staphylococcus aureus, Staphylococcus epidermidis (MRSA) and Gram-negative bacteria Escherichia coli ) as well as antiviral activity on ORF virus but it is still need further extensive studies for safety and drug interaction. Antimicrobial, Antiviral Activity and GC-MS Analysis of Essential IntroductionMedicinal plants still constitute one of the major source of drugs in modern as well as traditional medicine throughout the world [1,2]. Since a long period of time, plants have been a valuable source of natural products for maintaining animals and human health [2,3]. The development of multidrug-resistant(MDR) bacteria takes place because of the accumulation of different antibiotic resistance mechanisms inside the same strain which able to live in the presence of antibiotics drugs so that standard treatments become ineffective [4,[14][15][16]. Although, the pharmacological companies have produced a number of new antibiotics recently, but even then microorganism resistance to antibiotics increased throughout the world [5,7]. This situation has attract attention of researchers towards herbal products for developing and improve the antibacterial drugs quality [6,7].Natural products of higher plants may possess a new source of antimicrobial agents with possibly novel mechanisms for treatment of infectious diseases [8,9]. Medicinal plants contain active principles which can be used as alternative effective herbal dru...

show abstract

Section: Discussionsupporting

confidence: 77%

Antimicrobial, Antiviral Activity and GC-MS Analysis of Essential Oil Extracted from Achillea fragrantissima Plant Growing In Sinai Peninsula, Egypt

Zeedan¹

2014

J Microb Biochem Technol

View full text Add to dashboard Cite

show abstract

“…Several studies have investigated the similarities of UPEC and APEC in their serogroups, virulence genotypes and assignments to phylogenetic groups (Johnson et al, 1998(Johnson et al, , 2001aKaper et al, 2004;Mokady et al, 2005;Johnson et al, 2006a;Moulin-Schouleur et al, 2006). It has been proposed that poultry may be a candidate vehicle for E. coli capable of causing human urinary tract disease, based on research showing transmission of avian E. coli from poultry to humans or similarities between avian E. coli and UPEC (Levy et al, 1976;Linton et al, 1977;Ojeniyi, 1989;van den Bogaard et al, 2002). Although previous studies have compared the content of virulence genes between UPEC and APEC, similarities of expression of the specific virulence genes of UPEC and APEC in the same chicken challenge model and/or murine UTI model have not to our knowledge been reported before.…”

Section: Discussionmentioning

confidence: 99%

Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model

et al. 2009

View full text Add to dashboard Cite

Avian pathogenic Escherichia coli (APEC) and uropathogenic E. coli (UPEC) establish infections in extraintestinal habitats of different hosts. As the diversity, epidemiological sources and evolutionary origins of extraintestinal pathogenic E. coli (ExPEC) are so far only partially defined, in the present study,100 APEC isolates and 202 UPEC isolates were compared by their content of virulence genes and phylogenetic groups. The two groups showed substantial overlap in terms of their serogroups, phylogenetic groups and virulence genotypes, including their possession of certain genes associated with large transmissible plasmids of APEC. In a chicken challenge model, both UPEC U17 and APEC E058 had similar LD 50 , demonstrating that UPEC U17 had the potential to cause significant disease in poultry. To gain further information about the similarities between UPEC and APEC, the in vivo expression of 152 specific genes of UPEC U17 and APEC E058 in both a murine urinary tract infection (UTI) model and a chicken challenge model was compared with that of these strains grown statically to exponential phase in rich medium. It was found that in the same model (murine UTI or chicken challenge), various genes of UPEC U17 and APEC E058 showed a similar tendency of expression. Several iron-related genes were upregulated in the UTI model and/or chicken challenge model, indicating that iron acquisition is important for E. coli to survive in blood or the urinary tract. Based on these results, the potential for APEC to act as human UPEC or as a reservoir of virulence genes for UPEC should be considered. Further, this study compared the transcriptional profile of virulence genes among APEC and UPEC in vivo.

show abstract

“…Comparative analyses between APEC and human exPEC have revealed striking similarities in genomic islands, virulence genes, overlapping O serogroups and phylogeny (Stordeur et al, 2002;Schouler et al, 2004;Germon et al, 2005;Rodriguez-Siek et al, 2005b;Ron, 2006;Chouikha et al, 2006;Johnson et al, 2007a;Ewers et al, 2007;Kariyawasam et al, 2007). Furthermore, earlier work had shown APEC strains to be easily transmitted to humans (Linton et al, 1977;Ojeniyi, 1989). Indeed, studies have shown that some APEC strains could belong to the same clones as human exPEC strains (Achtman et al, 1986;White et al, 1993b).…”

Section: The Apec Pathotypementioning

confidence: 99%

Colibacillosis in poultry: unravelling the molecular basis of virulence of avian pathogenicEscherichia coliin their natural hosts

Dziva¹,

Stevens²

2008

Avian Pathology

299

222

View full text Add to dashboard Cite

Avian colibacillosis is caused by a group of pathogens designated avian pathogenic Escherichia coli (APEC). Despite being known for over a century, avian colibacillosis remains one of the major endemic diseases afflicting the poultry industry worldwide. Autologous bacterins provide limited serotype-specific protection, yet multiple serogroups are associated with disease, especially O1, O2 and O78 among many others. Experimental infection models have facilitated the identification of some key APEC virulence genes and have allowed testing of vaccine candidates. Well-recognized virulence factors include Type 1 (F1) and P (Pap/ Prs) fimbriae for colonization, IbeA for invasion, iron acquisition systems, TraT and Iss for serum survival, K and O antigens for anti-phagocytic activity, and a temperature-sensitive haemagglutinin of imprecise function. Intriguingly, these factors do not occur universally among APEC, suggesting the presence of multiple alternative mechanisms mediating pathogenicity. The recent availability of the first complete APEC genome sequence can be expected to accelerate the identification of bacterial genes expressed during infection and required for virulence. High-throughput molecular approaches like signature-tagged transposon mutagenesis have already proved invaluable in revealing portfolios of genes expressed by pathogenic bacteria during infection, and this has enabled identification of APEC O2 factors required for septicaemia in the chicken model. Complimentary approaches, such as in vivo-induced antigen technology, exist to define the activities of APEC in vivo. In recent years, reverse vaccinology and immuno-proteomic approaches have also enabled identification of novel vaccine candidates in other bacterial pathogens. Collectively, such information provides the basis for the development or improvement of strategies to control APEC infections in the food-producing avian species.

show abstract

The Colonization of the Human Gut by Antibiotic Resistant Escherichia coli from Chickens

Abstract: Antibiotic resistant Escherichia coli on a commercially prepared chicken carcass colonized the gut of a human volunteer handling the raw meat. Strains from both sources, identified on the basis of serotype and characterization of plasmids carried, were found to be identical.

Cited by 126 publications

References 13 publications

Antimicrobial, Antiviral Activity and GC-MS Analysis of Essential Oil Extracted from Achillea fragrantissima Plant Growing In Sinai Peninsula, Egypt

Antimicrobial, Antiviral Activity and GC-MS Analysis of Essential Oil Extracted from Achillea fragrantissima Plant Growing In Sinai Peninsula, Egypt

Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model

Colibacillosis in poultry: unravelling the molecular basis of virulence of avian pathogenicEscherichia coliin their natural hosts

Contact Info

Product

Resources

About