The colocalizations of pulp neural stem cells markers with dentin matrix protein-1, dentin sialoprotein and dentin phosphoprotein in human denticle (pulp stone) lining cells

Korkmaz, Yüksel; Imhof, Thomas; Kämmerer, Peer W.; Bloch, Wilhelm; Rink, Svenja; Möst, Tobias; Weber, Manuel; Kesting, Marco; Galler, Kerstin M.; Deschner, James

doi:10.1016/j.aanat.2021.151815

Cited by 8 publications

(11 citation statements)

References 72 publications

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“…Dental stem cells are very close to MSC in their morphology and surface markers. However, they express ectodermal markers such as a neuroepithelial stem cell protein, Nestin (NES), originally described as a specific glial marker [ 4 , 5 , 35 , 36 ]. Now it is assumed that NES is related to essential stem cell functions, including self-renewal/proliferation, differentiation, and migration [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

“…DMP1 and DSPP, classic odontoblastic markers, are expressed in odontoblasts, dentinal tubules. Their presence is necessary during dentine matrix mineralization [ 12 , 35 , 52 ]. The osteogenic potential of dental stem cells is probably one of the most important characteristics for their clinical application.…”

Section: Resultsmentioning

confidence: 99%

“…However, PDLSC have different embryonic origin: dental pulp is formed from dental papilla while PDLSC originate from dental follicle cells [ 7 , 72 ]. NES is considered as a marker not only of DPSC but also of odontoblasts and denticle lining cells, suggesting that denticle cells and odontoblast-like cells may derive from the same pulp stem cell populations [ 35 ]. Taking this into account, a greater tendency of DPSC to odontogenic differentiation in comparison with the PDLSC ( Figure 4 c,d) can be expected.…”

Section: Discussionmentioning

confidence: 99%

“…We observe odontoblastic markers only in samples differentiated from DPSC. DPSC are known to be capable of odontoblastic differentiation [ 14 , 15 , 55 ] and are also responsible for tertiary dentin formation [ 12 ] and denticle biomineralization [ 35 ]. PDLSC mechanism of osteogenic differentiation is not the same as in DPSC.…”

Section: Discussionmentioning

confidence: 99%

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Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome

et al. 2021

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Dental stem cells are heterogeneous in their properties. Despite their common origin from neural crest stem cells, they have different functional capacities and biological functions due to niche influence. In this study, we assessed the differences between dental pulp stem cells (DPSC) and periodontal ligament stem cells (PDLSC) in their pluripotency and neuroepithelial markers transcription, morphological and functional features, osteoblast/odontoblast differentiation and proteomic profile during osteogenic differentiation. The data were collected in paired observations: two cell cultures, DPSC and PDLSC, were obtained from each donor. Both populations had the mesenchymal stem cells surface marker set exposed on their membranes but differed in Nestin (a marker of neuroectodermal origin) expression, morphology, and proliferation rate. OCT4 mRNA was revealed in DPSC and PDLSC, while OCT4 protein was present in the nuclei of DPSC only. However, transcription of OCT4 mRNA was 1000–10,000-fold lower in dental stem cells than in blastocysts. DPSC proliferated at a slower rate and have a shape closer to polygonal but they responded better to osteogenic stimuli as compared to PDLSC. RUNX2 mRNA was detected by qPCR in both types of dental stem cells but RUNX2 protein was detected by LC-MS/MS shotgun proteomics only in PDLSC suggesting the posttranscriptional regulation. DSPP and DMP1, marker genes of odontoblastic type of osteogenic differentiation, were transcribed in DPSC but not in PDLSC samples. Our results prove that DPSC and PDLSC are different in their biology and therapeutic potential: DPSC are a good candidate for osteogenic or odontogenic bone-replacement cell-seeded medicines, while fast proliferating PDLSC are a prospective candidate for other cell products.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome

et al. 2021

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“…The healthy and inflamed human third molars were histopathologically characterized by hematoxylin and eosin (H&E) staining, as previously described [ 45 ]. The sections were first dried at room temperature for two hours.…”

Section: Methodsmentioning

confidence: 99%

Inflammation of the Human Dental Pulp Induces Phosphorylation of eNOS at Thr495 in Blood Vessels

et al. 2022

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The activity of endothelial nitric oxide synthase (eNOS) in endothelial cells increased with the phosphorylation of the enzyme at Ser1177 and decreased at Thr495. The regulation of the phosphorylation sites of eNOS at Ser1177 and Thr495 in blood vessels of the healthy and inflamed human dental pulp is unknown. To investigate this, healthy and carious human third molars were immersion-fixed and decalcified. The localization of eNOS, Ser1177, and Thr495 in healthy and inflamed blood vessels was examined in consecutive cryo-sections using quantitative immunohistochemical methods. We found that the staining intensity of Ser1177 in healthy blood vessels decreased in inflamed blood vessels, whereas the weak staining intensity of Thr495 in healthy blood vessels strongly increased in inflamed blood vessels. In blood vessels of the healthy pulp, eNOS is active with phosphorylation of the enzyme at Ser1177. The phosphorylation of eNOS at Thr495 in inflamed blood vessels leads to a decrease in eNOS activity, contributing to eNOS uncoupling and giving evidence for a decrease in NO and an increase in O2− production. Since the formation of the tertiary dentin matrix depends on intact pulp circulation, eNOS uncoupling and phosphorylation of eNOS at Thr495 in the inflamed pulp blood vessels should be considered during caries therapy.

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Irritation of Dental Sensory Nerves Promotes the Occurrence of Pulp Calcification

Zhan

Huang

Jiao

et al. 2023

Journal of Endodontics

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The colocalizations of pulp neural stem cells markers with dentin matrix protein-1, dentin sialoprotein and dentin phosphoprotein in human denticle (pulp stone) lining cells

Cited by 8 publications

References 72 publications

Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome

Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome

Inflammation of the Human Dental Pulp Induces Phosphorylation of eNOS at Thr495 in Blood Vessels

Irritation of Dental Sensory Nerves Promotes the Occurrence of Pulp Calcification

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